The relationship between STAT4 rs7574865 single nucleotide polymorphism and ANCA-associated vasculitis

doi:10.11958/20220821

Abstract

Abstract:

Objective To explore the association of STAT4 rs7574865 single nucleotide polymorphism with antineutrophil cytoplasmic autoantibody associated vasculitides (AAV). Methods A total of 145 patients with AAV (the AAV group) and 216 healthy controls (the control group) were included in this study. Whether the patients were accompanied by fever, proteinuria, hematuria and hemoptysis were collected. Multiplex PCR combined with high-throughput sequencing (MPCR-HTS) was used for genotyping. Genotypes, alleles and genotypes in different genetic models were compared between the two groups and between each clinical symptom subgroup. Logistic regression analysis was used to assess the relationship between genotype and risk of clinical symptoms. Results STAT4 rs7574865 genotype, allele and genotype distribution under different genetic models showed no significant difference between the two groups. There were no significant differences in genotype and allele distribution between different subgroups. In recessive model, the proportion of TT genotypes was lower in the hematuria group than that in the no hematuria group (P<0.05). Logistic regression analysis showed that TT genotype was not associated with the risk of hematuria. Conclusion STAT4 rs7574865 single nucleotide polymorphism was not associated with AAV susceptibility and clinical symptoms of fever, proteinuria, hematuria and hemoptysis.

Key words: anti-neutrophil cytoplasmic antibody-associated vasculitis, polymorphism, single nucleotide, hematuria, STAT4

CLC Number:

R593

Figures/Tables 4

References 26

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组别	n	基因型[例（%）]			等位基因[频数（%）]
组别	n	TT	GT	GG	T	G
对照组	216	17（7.9）	100（46.3）	99（45.8）	134（31.0）	298（69.0）
AAV组	145	17（11.7）	72（49.7）	56（38.6）	106（36.6）	184（63.4）
χ²		2.625			2.394

组别	n	基因型[例（%）]			等位基因[频数（%）]
组别	n	TT	GT	GG	T	G
对照组	216	17（7.9）	100（46.3）	99（45.8）	134（31.0）	298（69.0）
AAV组	145	17（11.7）	72（49.7）	56（38.6）	106（36.6）	184（63.4）
χ²		2.625			2.394

组别	n	隐性模型	显性模型	超显性模型
组别	n	TT/（GG+GT）	GG/（GT+TT）	（GG+TT）/GT
对照组	216	17/199	99/117	116/100
AAV组	145	17/128	56/89	73/72
χ²		1.510	1.842	0.392

组别	n	隐性模型	显性模型	超显性模型
组别	n	TT/（GG+GT）	GG/（GT+TT）	（GG+TT）/GT
对照组	216	17/199	99/117	116/100
AAV组	145	17/128	56/89	73/72
χ²		1.510	1.842	0.392

症状		n	基因型[例（%）]				等位基因[频数（%）]
症状		n	TT	GT	GG	χ²或P	T	G	χ²
血尿	无	38	1（5.9）	24（33.3）	13（23.2）	5.781	26（24.5）	50（27.2）	0.243
	有	107	16（94.1）	48（66.7）	43（76.8）	5.781	80（75.5）	134（72.8）	0.243
蛋白尿	无	40	6（35.3）	19（26.4）	15（26.8）	0.750^▲	31（29.2）	49（26.6）	0.230
	有	105	11（64.7）	53（73.6）	41（73.2）	0.750^▲	75（70.8）	135（73.4）	0.230
咯血	无	133	15（88.2）	68（94.4）	50（89.3）	0.433^▲	98（92.5）	168（91.3）	0.117
	有	12	2（11.8）	4（5.6）	6（10.7）	0.433^▲	8（7.5）	16（8.7）	0.117
发热	无	81	11（64.7）	35（48.6）	35（62.5）	3.075	57（53.8）	105（57.1）	0.296
	有	64	6（35.3）	37（51.4）	21（37.5）	3.075	49（46.2）	79（42.9）	0.296