Impact of loganin on pain response in rats with lumbar disc herniation by inhibiting CXCL12/CXCR4 signal pathway

doi:10.11958/20230249

Abstract

Abstract:

Objective To investigate the impact of loganin on pain response in rats with lumbar disc herniation (LDH) by regulating CXCL12/CXCR4 signal pathway. Methods Ten of the 50 rats were randomly selected as the sham group according to random number table method, and LDH rat model was established in other rats. Model rats were randomly divided into the model group, the CXCR4 antagonist group (AMD3100 group, intrathecal injection, 20 μg), the loganin group (5 mg/kg, intraperitoneal injection), and the loganin (5 mg/kg, intraperitoneal injection)+CXCL12 group (intrathecal injection, 250 ng), with 10 animals in each group. After the intervention according to drug groups, the latency of thermal foot withdrawal reaction and the threshold of mechanical foot withdrawal threshold were measured respectively. HE staining was applied to observe histomorphological changes of spinal dorsal horn. Levels of TNF-α, IL-1β and IL-6 in spinal dorsal horn were detected by ELISA. Immunofluorescence staining was applied to detect the activation of microglia. Western blot assay was used to detect the level of CXCL12/CXCR4 pathway related proteins. Results Compared with the sham group, the model group had severe spinal cord dorsal horn tissue injury, the latency of thermal foot withdrawal reaction and mechanical foot withdrawal threshold were lower, and levels of microglia, levels of TNF-α, IL-1β, IL-6, and protein expression levels of CXCL12, CXCR4, NLRP3, caspase-1 and IL-1β in spinal dorsal horn tissue were higher (P<0.05). Compared with the model group, the pathological injury of spinal dorsal horn tissue was reduced in the AMD3100 group and the loganin group. The latency of thermal foot withdrawal reaction and mechanical foot withdrawal threshold were higher, and levels of microglia, levels of TNF-α, IL-1β, IL-6, and the protein expression levels of CXCL12, CXCR4, NLRP3, caspase-1 and IL-1β in the spinal dorsal horn tissue were also lower (P<0.05). CXCL12 could weaken the improvement effect of loganin on pain response in LDH rats (P<0.05). Conclusion Loganin can improve the pain response of LDH rats, which may be related to the inhibition of CXCL12/CXCR4 signal pathway.

Key words: pain, intervertebral disc displacement, loganin, chemokine CXCL12, receptors, CXCR4

CLC Number:

R285.5

Figures/Tables 7

References 18

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组别	建模后1 d	治疗后3 d	治疗后7 d	治疗后14 d
Sham组	16.21±1.47	17.27±1.54	17.36±1.63	16.89±1.74
Model组	8.31±1.22^a	7.54±1.03^a	7.36±1.12^a	7.98±1.05^a
AMD3100组	8.42±1.21^a	9.15±0.87^b	10.23±1.15^b	12.32±1.26^b
马钱子苷组	8.56±1.16^a	9.23±0.98^b	10.45±1.02^b	13.02±1.61^b
马钱子苷+ CXCL12组	7.87±0.96^a	8.05±0.74	8.32±0.62^c	10.14±1.10^c
F	85.440^＊＊	139.789^＊＊	115.357^＊＊	58.628^＊＊

组别	建模后1 d	治疗后3 d	治疗后7 d	治疗后14 d
Sham组	16.21±1.47	17.27±1.54	17.36±1.63	16.89±1.74
Model组	8.31±1.22^a	7.54±1.03^a	7.36±1.12^a	7.98±1.05^a
AMD3100组	8.42±1.21^a	9.15±0.87^b	10.23±1.15^b	12.32±1.26^b
马钱子苷组	8.56±1.16^a	9.23±0.98^b	10.45±1.02^b	13.02±1.61^b
马钱子苷+ CXCL12组	7.87±0.96^a	8.05±0.74	8.32±0.62^c	10.14±1.10^c
F	85.440^＊＊	139.789^＊＊	115.357^＊＊	58.628^＊＊

组别	建模后1 d	治疗后3 d	治疗后7 d	治疗后14 d
Sham组	27.45±1.48	28.15±1.56	28.54±1.67	28.74±1.83
Model组	10.56±0.83^a	10.24±0.91^a	11.15±1.06^a	11.34±1.42^a
AMD3100组	10.72±0.78^a	13.26±0.84^b	17.64±1.32^b	22.56±1.93^b
马钱子苷组	10.89±0.90^a	13.58±0.87^b	18.25±1.43^b	23.43±2.12^b
马钱子苷+ CXCL12组	10.31±0.72^a	12.29±0.76	13.60±1.05^c	15.23±1.34^c
F	588.603^＊＊	487.330^＊＊	251.968^＊＊	156.140^＊＊

组别	建模后1 d	治疗后3 d	治疗后7 d	治疗后14 d
Sham组	27.45±1.48	28.15±1.56	28.54±1.67	28.74±1.83
Model组	10.56±0.83^a	10.24±0.91^a	11.15±1.06^a	11.34±1.42^a
AMD3100组	10.72±0.78^a	13.26±0.84^b	17.64±1.32^b	22.56±1.93^b
马钱子苷组	10.89±0.90^a	13.58±0.87^b	18.25±1.43^b	23.43±2.12^b
马钱子苷+ CXCL12组	10.31±0.72^a	12.29±0.76	13.60±1.05^c	15.23±1.34^c
F	588.603^＊＊	487.330^＊＊	251.968^＊＊	156.140^＊＊

组别	TNF-α	IL-1β	IL-6
Sham组	95.24±10.21	56.43±8.34	35.34±5.61
Model组	274.36±42.31^a	149.54±12.42^a	185.42±32.41^a
AMD3100组	132.46±16.55^b	92.35±8.75^b	65.33±7.32^b
马钱子苷组	127.48±15.28^b	95.36±9.82^b	70.25±8.14^b
马钱子苷+ CXCL12组	183.14±20.42^c	112.47±10.23^c	131.46±12.45^c
F	86.880^＊＊	114.180^＊＊	133.820^＊＊