The effect of electroacupuncture on paclitaxel-induced neuropathic pain in rats

doi:10.11958/20240609

Abstract

Abstract:

Objective To observe the effect of electroacupuncture (EA) on the expression of NKCC1, KCC2 and activation of microglia in spinal dorsal horn of paclitaxel (PTX)-induced neuropathic pain rats and its possible mechanism. Methods Male SD rats were randomly divided into the vehicle group (vehicle), the PTX group, the PTX + EA group and the PTX + sham EA group, with 12 rats in each group. The rat model of PTX-induced neuropathic pain was established by intraperitoneal injection of PTX. After modeling, EA was applied to "Zusanli" and "Yanglingquan" for 7 days in the PTX + EA group. Paw withdrawal threshold and paw withdrawal latency were tested at 2 days before and 1, 3, 5, 7, 14 and 21 days after PTX injection. Immunofluorescence and Western blot assay were used to detect expression levels of sodium-potassium-chloride cotransporter 1 (NKCC1), potassium-chloride cotransporters 2 (KCC2) and microglia markers - ionized calcium binding adapter molecule 1 (Iba1) in spinal dorsal horn. Results Compared with the vehicle group, mechanical and thermal hyperalgesia of both hind feet were found in the PTX group, and the expression of NKCC1 and the number of activated microglia in dorsal horn tissue of spinal cord were increased. Compared with the PTX group, mechanical and thermal hyperalgesia were significantly improved in the PTX+EA group at day 14 and 21, and the expression levels of NKCC1 and Iba1 in dorsal horn tissue of spinal cord were decreased. There was no significant difference in KCC2 expression between the four groups. Conclusion Electroacupuncture can effectively relieve paclitaxol-induced neuropathic pain, which may be related to the inhibition of NKCC1 expression and microglia activation in spinal dorsal horn of rats.

Key words: electroacupuncture, paclitaxel, neuralgia, spinal cord dorsal horn, microglia, sodium-potassium-chloride symporters, neuropathic pain

CLC Number:

R245

Figures/Tables 8

References 22

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组别	第-2天	第-1天	第1天	第3天	第5天	第7天	第14天	第21天
Vehicle组	23.72±4.05	24.11±3.09	24.85±3.90	22.85±3.90	22.46±4.57	22.85±3.90	24.11±3.09	24.11±3.09
PTX组	24.11±3.09	22.46±4.57	21.88±5.72	14.73±3.73^a	12.41±3.59^a	10.84±2.11^a	8.33±2.96^a	10.36±3.79^a
PTX+EA组	25.37±0.00	21.88±5.72	22.64±6.69	13.71±5.83^a	11.48±1.22^a	9.08±3.74^a	14.90±3.20^ab	20.91±6.91^b
PTX+Sham EA组	24.11±3.09	22.21±5.04	22.21±5.04	12.62±3.39^a	12.29±3.32^a	11.11±4.12^a	8.88±2.46^a	9.41±4.08^a

组别	第-2天	第-1天	第1天	第3天	第5天	第7天	第14天	第21天
Vehicle组	23.72±4.05	24.11±3.09	24.85±3.90	22.85±3.90	22.46±4.57	22.85±3.90	24.11±3.09	24.11±3.09
PTX组	24.11±3.09	22.46±4.57	21.88±5.72	14.73±3.73^a	12.41±3.59^a	10.84±2.11^a	8.33±2.96^a	10.36±3.79^a
PTX+EA组	25.37±0.00	21.88±5.72	22.64±6.69	13.71±5.83^a	11.48±1.22^a	9.08±3.74^a	14.90±3.20^ab	20.91±6.91^b
PTX+Sham EA组	24.11±3.09	22.21±5.04	22.21±5.04	12.62±3.39^a	12.29±3.32^a	11.11±4.12^a	8.88±2.46^a	9.41±4.08^a

组别	第-2天	第-1天	第1天	第3天	第5天	第7天	第14天	第21天
Vehicle组	12.17±0.68	12.53±0.72	12.07±0.83	11.72±0.81	12.37±0.46	12.28±0.68	12.18±1.03	12.27±1.00
PTX组	12.78±0.29	12.59±1.07	12.25±0.82	8.98±0.24^a	7.03±0.77^a	5.57±0.37^a	5.21±1.19^a	5.65±0.36^a
PTX+EA组	12.60±0.60	12.55±0.83	11.14±1.26	9.16±0.38^a	6.70±0.50^a	6.00±0.77^a	10.31±1.15^b	11.47±0.58^b
PTX+Sham EA组	12.92±0.51	11.68±0.60	11.35±0.63	8.92±0.15^a	6.61±0.41^a	5.21±0.35^a	5.23±0.87^a	5.80±0.64^a

组别	第-2天	第-1天	第1天	第3天	第5天	第7天	第14天	第21天
Vehicle组	12.17±0.68	12.53±0.72	12.07±0.83	11.72±0.81	12.37±0.46	12.28±0.68	12.18±1.03	12.27±1.00
PTX组	12.78±0.29	12.59±1.07	12.25±0.82	8.98±0.24^a	7.03±0.77^a	5.57±0.37^a	5.21±1.19^a	5.65±0.36^a
PTX+EA组	12.60±0.60	12.55±0.83	11.14±1.26	9.16±0.38^a	6.70±0.50^a	6.00±0.77^a	10.31±1.15^b	11.47±0.58^b
PTX+Sham EA组	12.92±0.51	11.68±0.60	11.35±0.63	8.92±0.15^a	6.61±0.41^a	5.21±0.35^a	5.23±0.87^a	5.80±0.64^a

组别	NKCC1	KCC2	Iba1
Vehicle组	1.00±0.07	1.00±0.06	1.00±0.23
PTX组	4.35±0.16^a	1.05±0.08	3.72±0.18^a
PTX+EA组	1.73±0.07^b	1.02±0.35	1.69±0.21^b
PTX+Sham EA组	3.76±0.63^a	0.99±0.16	3.70±0.32^a
F	70.440^＊＊	0.246	101.500^＊＊