Abstract: Abstract：Objective To study the percutaneous permeability through mouse skin of lidocaine hydrochloride-loaded destran-based niosomes（LID-HLD-BNs）in vitro and in vivo. Methods HPLC was employed to exam lidocaine hydrochlo⁃ ride. Lidocaine hydro-chloride-loaded conventional liposomes (LID-CLs) and lidocaine hydrochloride injection (LID-IJ) were used as control. Isolated mouse skin was added into Franz diffusion cell to evaluate the permeability of LID-HLD-BNs in vitro. Confocal Laser Scanning Microscopy（CLSM）was used to observe the permeation depth of mouse skin in vivo. Re⁃ sults The permeation rate and cumulative permeation amount were significantly higher in LID-HLD-BNs group than those of LID-CLs and LID-IJ groups (P＜0.05). CLSM studies also confirmed that HLD-BNs reached deeper layers of the skin. Conclusion LID-HLD-BNs has good transdermal ability.

Key words: administration, cutaneous；liposomes；lidocaine；chromatography, liquid；drug utilization, in vitro