Abstract: Abstract: Objective To study the impacts of alcohol dependence on the anticonvulsant effect of diazepam. Meth⁃ ods Kunming mice (n=36) were divided into 3 groups (n=12 in each group), Alcohol Dependence Group（A group）, Diaze⁃ pam Group（D group）and Normal Saline Group（N group）. A group received an intraperitoneal injection with a 0.2 mL dose of 0.8% alcohol in NS (normal saline) , while both D and N group received an injection with a 0.2 mL dose of NS without alco⁃ hol , twice a day. Mice’s autonomic activities were monitored every day. After 7 days, the electroconvulsive experiment was performed. Both A and D group were given a weight-based dose of 0.05 mL/10 g of 0.05% diazepam via intraperitoneal injection, while N group was given a 0.05 mL/10 g dose of NS. Before administration and after 15, 30, 60 min of administration, the convulsion threshold of each group was measured. Results The count of autonomic activity of mice in A group was less than that of mice in D and N group during the 2nd day to 6th day（P＜0.05）. On the 1st and 7th day, the difference of the count of autonomic activity of mice between A group and the other two groups was not statistically significant（P > 0.05）. The convulsion threshold of mice in A group was higher than that of mice in D and N group before administration（P＜0.05）. Af⁃ ter administration, the convulsion threshold of mice in N group didn’t show statistically significant difference from that of mice before administration（P > 0.05）. After 15 min of administration, the convulsion threshold of mice in D group was high⁃ er than that of mice in A and N group（P＜0.05）, while the convulsion threshold of mice in A group was higher than that of mice in N group（P＜0.05）. After 30 min and 60 min of administration, both the convulsion thresholds of mice in A and D group were higher than that of mice in N group（P＜0.05）. However, at this point, the difference of the convulsion thresholds of mice between A and D group was not statistically significant（P > 0.05）. Conclusion Alcohol dependence has anticon⁃ vulsant effect. Alcohol dependence weakens the anticonvulsant effect of diazepam.

Key words: alcohol dependence, diazepam, anticonvulsant action；electric stimulation；gamma-aminobutyric acid