Abstract:

[Abstract] Objective To build rat DVT inferior vena cava partial stasis (narrow) model, to detected the expression of β2-GP1, VEGF and TF in rat blood, and to investigat the correlation between β2-GP1, VEGF and TF with DVT. Methods SD rats (n=70) are divided into control group (n = 10), sham operation group (n = 30) and the model group (n = 30) ran? domly and DVT model was built by the inferior vena cava partial stasis (narrow) after 2 h, 8 h and 24 h respectively. In each time point, ten rats were taken in each group, inferior vena cava blood were collected while β2-GP1, VEGF and TF expres? sion were detected by ELISA. Results In rat experiment, compared with control group, there was no significant change in? expression of β2-GP1, VEGF and TF in sham operation group (P ＞ 0.05). Levels of β2-GP1, VEGF and TF were in? creased at the 2nd hour and 8th hour then peak at the 24th hour which was higher than those in the 24th hour control group and in Sham group and it was also higher than those in the 2nd hour and the 8th hour in model group with statistical signifi? cant difference (P ＜ 0.01). Conclusion Based on the above experimental data, in rat DVT formation process, β2-GP1, VEGF and TF may play an important role in promote DVT formation.

Key words: β-2 glycoprotein 1, VEGF, Deep venous thrombosis, thromboplastin, venous thrombosis, rats, Sprague-Dawley