Tianjin Medical Journal

Tianjin Medical Journal ›› 2022, Vol. 50 ›› Issue (6): 595-600.doi: 10.11958/20212614

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Relationship between miR-27a expression and ferroptosis in the early stage of ischemic stroke of rats

ZHANG Jing, SUN Hui, ZHU Lijun, FENG Ziren, DU Lin, MENG Aiguo△   

  1. North China University of Science and Technology Affiliated Hospital, Key Laboratory of Medical Molecular Testing and Diagnosis in Tangshan, Tangshan 063000, China
  • Received:2021-11-24 Revised:2022-03-05 Published:2022-06-15 Online:2023-12-20

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Abstract: Abstract: Objective　To investigate the expression of miR-27a in the early stage of ischemic stroke in rats and its relationship with ferroptosis. Methods　A total of 88 SD rats were randomly divided into (1) the control group, the sham group, the ischemia group (6, 12, 24 and 48 h groups according to ischemia time); (2) the ischemia 48 h group (I 48 h group), the solvent control group (I 48 h+DMSO group), the ischemia 48 h+Ferrostatin-1 group (I 48 h+Fer-1 group); (3) the I 48 h+negative control (I 48 h+NC) group, the I 48 h+miR-27a agonist group (I 48 h+ago-miR-27a group) and the I 48 h+miR-27a antagonist group (I 48 h+antago-miR-27a group). The brain tissue was separated after treatment. TTC staining was used to observe the size of cerebral infarction. The expression of glutathione peroxidase 4 (GPX4) was analyzed by Western blot assay. The glutathione (GSH), iron and methylene dioxyamphetamine (MDA) contents were detected by detection kits. The expression of miR-27a was analyzed by qPCR. Results　(1) Compared with the sham group, the expression of miR-27a and the contents of iron and MDA were increased in the ischemia group, while the expressions of GPX4 and GSH were decreased (P＜0.05), which were the most significant at 48 h of ischemia. (2) Compared with the I 48 h+DMSO group, the cerebral infarct size was decreased in the I 48 h+Fer-1 group, the expression levels of GPX4 and GSH were increased, while the contents of iron and MDA were decreased (P＜0.05). (3) Compared with the I 48 h+NC group, the cerebral infarct area and miR-27a expression were increased in the I 48 h+ago-miR-27a group, while the expressions of GPX4 and GSH were decreased, and the contents of iron and MDA were increased (P＜0.05). However, the above parameters showed opposite changes in the antago-miR-27a group. Conclusion　The increased expression of miR-27a in the early stage of ischemic stroke in rats can aggravate the damage of ischemic brain injury by promoting the occurrence of ferroptosis.

Key words: stroke, rats, Sprague-Dawley, miR-27a, ferroptosis