Abstract: Objective To observe the effect of estrogen on modulating apolipoprotein AⅠ gene transcription in HepG2 cells. Methods Exposure HepG2 cells to different concentration of estradiol (0.01,0.1,1.0,10.0,20.0 μmol/L) for 24 hours, respectively; HepG2 cells were incubated with estradiol at 10μmol/L concentration for 1,6,24,48 hour. RNA was extracted from HepG2 cells collected at above time points and the expression levels of apoAⅠmRNA was analysized by RT-PCR. Results The level of apoAⅠmRNA expressed in HepG2 cells stimulated by different concentrate of estradiol was in a dose-dependent increased. Compared with 0.01, 0.1, 1μmol/L of estradiol, apoAⅠ mRNA was significantly higher in 10μmol/L of estradiol; A time-course experiment at the 10μmol/L of estradiol indicated that the expression of apoAⅠmRNA was significantly increased at 6 hour(P<0.05)and the highest at 24 hour(P<0.05). Conclusions Estrogen may play an important role in the transcription of apoAⅠgene, the effect for mRNA expression show a dose-dependent and time-dependent partly.

Key words: estrogen、apolipoprotein AⅠ、RT-PCR