Abstract: The effect of Akt1 and Akt2 gene transfection on invasion and migration of human gastric epithelial cell GES-1 XIE jia-bei, ZHANG Qing-yu*, HAN jing ,WANG tao, ZHANG jie. Department of Gastroenterology, Tianjin Medical University General Hospital, Tianjin 300052,China Kang Chun-sheng, Department of Neurosurgery, Tianjin Medical University General Hospital and Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin 300052,China. Corresponding author : ZHANG Qing-yu, Email: zhangqy@tijmu.edu.cn Abstract To observe the effects of Akt1 and Akt2 gene transfections on invasion and migration of human gastric epithelial cell line GES-1. Methods The plasmid of p-LXSN-Akt1 which contains the whole Akt1 gene sequence and p-LXSN-Akt2 which contains the whole Akt2 gene sequence were used to transfect GES-1 cells with lipofectamine, The GES-1 cells which were transfected by the empty vector p-LXSN were grouped as the black vector ,the no transfected ones were the control. Then the positive cell clones were selected with antibiotics G418. The protein expressions of Akt1, Akt2 and Mmp-2 were measured by Western blotting analysis; and Transwell assay analyzed their invasion and metastasis capability. The expressions of F-actin were detected by Immunofluorescence. Results Cell modes which expresses Akt1 gene (Akt1 group) and which expresses Akt2 gene (Akt2 group) were obtained; Compared with the black vector group and control group, The protein expressions of Mmp-2 in Akt1 and Akt2 groups were increased significantly.（P<0.01; transwell assay showed Akt1 and Akt2 groups could significantly advance the invasion and metastasis of cells; the expressions of F-actin were increased corresponding1y in Akt1 and Akt2 groups. Conclusion Akt1, Akt2 gene could increase cell invasion and migration, which showed the promotion of malignant transformation of GES-1 cells .

Key words: Akt1 gene, Akt2 gene, GES-1, invasion, migration