Abstract:

[Abstract]   Objective  Toobserve the effects of different doses of Peng-Hui on expression levels of insulin receptor
substance1(IRS-1) in type 2diabetes (T2DM) rats.Methods  T2DM rat model was established by feeding with high-fatdiet and intraperitoneal injecting streptozotocin (STZ). T2DM model rats were randomized into four groups: T2DM control group, high dose Peng-Hui (150mg/kg) group, medium dose Peng-Hui (100mg/kg) group and low dose Peng-Hui (50mg/kg) group. The T2DM control group was fed with only high-fat-diet. The normal control group was fed with common diet. The expression levels of IRS-1in pancreas and skeletal muscle tissues were detected by immunohistochemistry method after 8-week treatment, and fasting blood glucose (FBG) and insulin levels were also detected in five groups.Results   FBG levels were significantly lower in medium dose Peng- Hui group than those of T2DM control group and low dose Peng- Hui group (P<0.05). There were no significant differences in insulin levels between high, medium and low dose Peng-Hui groups and T2DM control group (P＞0.05). The expression levels of IRS-1of pancreas and skeletal muscle tissues were significantly lower in high dose Peng-Hui group than that of T2DM control group (P <0.05). Conclusion  The expression of IRS-1 was decreased in high dose Peng-Hui group, but no effects on FBG and plasma insulin.

Key words: diabetes mellitus, type 2, pancreas, muscle, skeletal, blood glucose, insulin, 大鼠