Abstract:

[Abstract] Objective To investigate the effect of cisplatin on proliferation and invasion of colorectal cancer cell line SW480. Methods SW480cells were used as the research object, and untreated SW480cells were used as the control group. Different concentrations of cisplatin was given at different times to intervene. The MTT, Transwell chamber and phos? phate determination methods were used to detect proliferation, invasion and Na + - K + - ATPase activity expression level in SW480cells.Results The physiological concentration of cisplatin (70µmol/L) inhibited the proliferation of SW480cells at 48h. There was no significant difference in the inhibition rate at48h compared with72h and96h. Treatment with70 µmol/L cisplatin for48h reduced the number of cells through Matrigel membrane matrix. The Na + -K + -ATPase activity was significantly increased in 35, 70and140µmol/L cells after treatment with17.5, 35, 70and140µmol/L of cisplatin in SW480cells for48h, and Na + -K + -ATPase reached the highest level at70µmol/L of cisplatin.Conclusion The decreased activity of Na + -K + -ATPase may lead to the attenuation in proliferation and invasion of colorectal cancer cells, which may be associated with cisplatin resistance in colorectal cancer cell SW480.

Key words: colorectal cancer, cisplatin, proliferation, Transwell