Tianjin Medical Journal

Tianjin Med J ›› 2016, Vol. 44 ›› Issue (1): 19-22.doi: 10.11958/20150161

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T Endothelial progenitor cells (EPCs) and promote angiogenesis factor levels in peripheral blood in patients with obstructive sleep apnea

XUE Yanchao1 , SUN Bei 2 , WANG Xin1 , FENG Jing1△, CAO Jie1△   

  1. Abstract： Objective To explore the repair possibilities of endothelial progenitor cells (EPCs）in peripheral blood in patients with different extents of obstructive sleep apnea (OSA) through measuring the levels of pro-angiogenic factors and different subgroups EPCs in peripheral blood in patients with OSA. Methods Ninety adult patients with OSA, 30 healthy controls with matched age and gender were enrolled for this study. The subjects performed Polysomnography, were divided in? to four group based on Apnea Hypopnea Index (AHI). The serum levels of HIF-1α, SDF-1α and VEGF were assessed by ELISA. Mononuclear cells were isolated from peripheral blood with density gradient centrifugation, and flow cytometry was used to detect levels of CD133+ KDR+ EPC, CD133+ CD34+ EPC, CD34+ KDR+ EPC and ALDHlo CD34+ KDR+ EPC based on AL? DH activity, and CD133, CD34, PE-KDR related cell surface markers. Results The levels of CD133+ KDR+ EPC, CD133+ CD34+ EPC, CD34+ KDR+ EPC were higher in OSA groups than those of control group, both of which were higher in severe OSA group than those of in mild and moderate OSA groups. The levels of ALDHlo CD34+ KDR+ EPC were higher in mild and moderate OSA groups than that of the control groups, and the levels of ALDHlo CD34+ KDR+ EPC were significantly lower in se? vere OSA group than those of control, mild and moderate OSA groups. Serum levels of HIF-1α. VEGF were significantly high? er in OSA groups compared to those in control groups, both of which were higher in severe OSA group than those of mild and moderate OSA groups. Serum levels of SDF-1α were significantly lower in severe OSA groups than those of mild, moderate OSA and control groups (P < 0.05). Conclusion The mobilization and recruitment of different subtypes of EPCs are obvious? ly increased in patients with OSA, but ALDHlo CD34+ KDR+ EPC with vascular repair capacity keeps to invariability, even de?creases in patients with severe OSA, which results in endothelial damage, and increases the risk of cardiovascular disease.
  • Received:2015-09-15 Revised:2015-10-15 Published:2016-01-15 Online:2016-01-15
  • Contact: △Corresponding Author E-mail: zyyhxkfj@126.com; tjcaojie@sina.com E-mail:xych1213@126.com

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Abstract: [Abstract] Objective Hypoxia is one of critical pathophysiological element in obstructive sleep apnea (OSA). Intermittent hypoxia (IH) induced endothelial cells, and enhanced the activity of promoting vascular growth factor. Endothelial progenitor cells (EPCs) of mobilization and recruitment repaired damaged vascular. EPC with low acetaldehyde dehydrogenase (ALDH) activity (ALDHlo EPC) showed the reparation ability. In this study, measuring the serum levels of hypoxia-inducible factor-1α (HIF-1?), plasma stromal cell-derived factor-1α (SDF-1?), vascular endothelial growth factor (VEGF) and levels of different subgroups EPC in peripheral blood in patients with OSA. To explore the possibility of EPC in peripheral blood repaired vascular in patients with different extent OSA. Methods 90 adult patients with OSA, 30 healthy controls with matched age, gender were enrolled for this study. The subjects performed Polysomnography, and were divided into four groups based on Apnea Hypopnea Index (AHI). they are mild OSA group, moderate OSA group, severe OSA group and control group respectively, and including 30 patients in each group. Measuring the serum levels of HIF-1?、SDF-1? and VEGF by ELISA. Mononuclear cells were isolated from peripheral blood with density gradient centrifugation, and based on ALDH activity and CD133, CD34, KDR appropriate cell surface markers by flow cytometry (FACS ) to detected the levels of CD133+ EPC、CD133+CD34+ EPC、CD34+ EPC and ALDHloCD34+ EPC. Results The levels of CD133+ EPC、CD133+CD34+ EPC、CD34+ EPC of patients with OSA were increased compared to those in healthy controls (79.08±12.52、31.91±8.89、86.09±17.03, 97.34±16.18、45.19±18.23、123.40±32.73, 119.20±45.50、76.65±31.91、239.40±87.23 vs 51.90±18.85、18.71±8.50、53.29±18.78), but the levels of ALDHlo EPC in mild and moderate OSA groups were increased than those in the controls, and the levels of ALDHlo EPC were significantly decreased in severe OSA group (37.69±11.16、29.52±11.15、13.01± 6.36 vs 20.45± 8.99). Serum levels of HIF-1?、VEGF in patients with OSA were significantly increased compared to those in healthy controls (1.70±0.15、53.29±6.57,1.87±0.35、98.00±7.00,2.56±0.26、155.60±12.80 vs 1.30±0.21、36.79±5.59) but serum levels of SDF-1? were significantly decreased(1971.00±275.50、1587.00±241.70、1180.00±313.20 vs 2173.00±316.50) (P<0.05). Conclusion With increasing severity of OSA , Endothelial progenitor cells (EPCs) levels of mobilization and recruitment have increased, and ALDHlo EPC with vascular repair capacity were decreased, which results in endothelial damage, and increased the risk of cardiovascular disease.

Key words: [Key words] obstructive sleep apnea, HIF-1?, SDF-1?, VEGF, intermittent hypoxia, endothelial progenitor cells (EPCs), acetaldehyde dehydrogenase