Abstract: Objective　To study the effects of different stimulation patterns on the pattern visual evoked potentials (PVEP) in mice with implanted electrodes onto the brain surface. Methods　Twenty-two 6-week-old C57BL/6J mice were anesthetized and their heads were fixed on a stereotactic instrument. Recording electrodes were implanted onto the surface of the binocular area of the primary visual cortex, and the reference electrodes were implanted below the nasal bone. Three different forms of pattern visual stimulation were performed on each anaesthetized mouse, which were horizontal grating stimulation, vertical grating stimulation and reversal checkerboard stimulation. P100 waveform amplitudes and peak times of PVEP recorded under different pattern visual stimuli were compared. Results　Under the same spatial frequency and temporal frequency, there were significant differences in the amplitudes (F=18.670, P＜0.01) and peak times (F=21.170，P＜0.01) of PVEP P100 waveforms evoked by horizontal grating stimulation (173.18 μV±33.00 μV, 87.59 ms±12.40 ms), vertical grating stimulation (189.05 μV±31.79 μV, 78.68 ms±9.50 ms) and reversal checkerboard stimulation (142.68 μV±32.64 μV, 96.55 ms±17.57 ms). There were statistically significant differences in all the pairwise comparisons of the amplitudes and peak times of the PVEP P100 waveforms (P＜0.05). The coefficients of variation of the three groups of data were 19.06%, 16.82% and 22.87%, and 14.16%, 12.07% and 18.19%, respectively. Conclusion　Vertical grating stimulation can be used to evoke pattern visual evoked potentials more sensitive and more stable than reversal checkerboard stimulation and horizontal grating stimulation.