Abstract: Objective　To study the effects of portoenterostomy (Kasai surgery) on liver transplantation (LT) for children with biliary atresia (BA). Methods　The clinical data of 880 children who received liver transplantation for BA in our hospital were retrospectively analyzed. According to whether Kasai surgery was performed before liver transplantation, they were divided into the Kasai group (n=542) and the non-Kasai group (n=338). The differences in general information, incidence of postoperative complications, graft survival rate and patient survival rate were compared between the two groups. Results　The months of age at the time of transplantation, height, body weight, graft cold ischemia time, graft quality and operation time of children were higher or longer in Kasai group than those in non-Kasai group, while the graft-recipient mass ratio, preoperative Child score and pediatric model of end-stage liver disease (PELD) score were lower in the Kasai group than those in the non-Kasai group (P＜0.05). There were no significant differences in gender, graft hot ischemia time, blood loss/body weight, intraoperative blood transfusion volume, duration of no-liver phase, duration of ventilator use, ICU hospitalization and postoperative hospitalization duration between the two groups. There were no significant differences in the outflow tract obstruction after operation, portal vein anastomotic stenosis, portal vein thrombosis, hepatic artery thrombosis, biliary leakage, biliary tract stenosis, lymphatic leakage, gastrointestinal fistula, intestinal obstruction, acute rejection, cytomegalovirus and Epstein-Barr virus infection between the two groups. There were no significant differences in graft survival rate and cumulative survival rate at 1 and 5 years after operation between the two groups. Conclusion　Liver transplantation is a safe and effective surgical method for the treatment of children with BA. Kasai surgery can postpone the time of liver transplantation in children, and have no effects on liver transplantation, postoperative graft survival, survival rate of children and the occurrence of major postoperative complications.