Effects of Bazhen Decoction combined with Baizhu Fuzi Decoction on glucose metabolism and estrogen levels in rats with polycystic ovary syndrome

doi:10.11958/20230549

Abstract

Abstract:

Objective To study the regulation effects of Bazhen Decoction combined with Baizhu Fuzi Decoction on glucose metabolism and estrogen level in rats with polycystic ovary syndrome. Methods The rat model of polycystic ovary syndrome was established by subcutaneous injection of human chorionic gonadotropin (HCG) combined with insulin. Rats were randomly divided into the polycystic ovary syndrome group, the Baizhu Fuzi Tang group (6.4 g/kg), the Bazhen Tang group (9.2 g/kg), the Bazhen Tang combined with Baizhu Fuzi Tang group (11.55 g/kg) and the diethylstilbestrol group (0.5 mg/kg). A blank control group (unmodulated rats) was set up with 10 rats in each group. The uterine index of rats was determined and calculated. Fasting blood glucose (FBG), 2 h postprandish blood glucose (2 h PBG) and fasting insulin (FINS) were determined and insulin sensitivity index (ISI) and insulin resistance index (IR) were calculated. Serum estradiol (E₂), testosterone (T), luteinizing hormone (LH), follicle stimulating hormone (FSH), prolactin (PRL), anti-Mullerian tube hormone (AMH) and insulin-like growth factor 1 (IGF-1), C-reactive protein (CRP), interleukin (IL)-6, tumor necrosis factor (TNF)-α, total peroxidase activity (T-AOC), superoxide dismutase (SOD) and malondialdehyde (MDA) levels in ovarian tissue were detected by enzyme-linked immunosorbent assay (ELISA). The pathological changes of ovarian tissue were detected by hematoxylin-eosin (HE) staining. Real-time quantitative polymerase chain reaction (qPCR) was used to determine mRNA levels of ARA70, CBP, SCR1 and HOXA10 in endometrium and mRNA expressions of AMPK, GLUT4 and PPARγ in ovarian tissue. Western blot assay was used to determine expression levels of AMPK, GLUT4 and PPARγ in ovarian tissue. Results Compared with the polycystic ovary syndrome group, uterine index, FINS, FBG, 2 h PBG, IR levels, serum T, LH, FSH, PRL, AMH, IGF-1 levels, ovarian tissue CRP, IL-6, TNF-α, T-AOC and MDA level, endometrial ARA70, CBP, SCR1 mRNA level of rats decreased significantly in the Baizhu Fuzi decoction group, the Bazhen decoction group and the Bazhen decoction combined with Baizhu Fuzi decoction group (P<0.05). ISI level, serum E₂ level, ovarian tissue SOD level, endometrial HOXA10 mRNA level, ovarian tissue AMPK, GLUT4 and PPARγ mRNA and protein levels were significantly increased (P<0.05). The above indexes were significantly changed in the Bazhen decoction and Bazhu Fuzi decoction group than those of the Bazhu Fuzi decoction group and the Bazhen decoction group (P<0.05). Conclusion Bazhen Decoction combined with Baizhu Fuzi Decoction can regulate glucose metabolism, inhibit ovarian tissue oxidation and inflammatory damage, improve endometrium tolerance, regulate estrogen level, and improve the progression of polycystic ovary syndrome in rats. The mechanism may be related to the regulation of AMPK/GLUT4/PPARγ pathway.

Key words: Zhu Fu Tang, Ba Zhen Tang, polycystic ovary syndrome, estrogens, glucose metabolism, Bazhen Decoction combined with Baizhu Aconite Decoction

CLC Number:

R285.5

Figures/Tables 9

References 19

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基因名称	引物序列（5′→3′）	产物大小/ bp
子宫内膜
ARA70	上游：TTGCTACTGGAACAGGGACAC	283
	下游：CCCGTGTGTTAGTTCTGCTGT	283
CBP	上游：CTCCCCAAGTTGCTGA	191
	下游：CCCCTGTAGCGGGCAAA	191
SCR1	上游：AAGTATGGGTGGATGGAGTTCAG	143
	下游：ATGAGGTGCTAAGTGTCTTTCAG	143
HOXA10	上游：CCCACCAACATACAAAGCAG	172
	下游：TGAGCATAGCAAAGGTCAGG	172
GAPDH	上游：AGGTTGTCTCCTGCGACTTCA	161
	下游：CTGTTGCTGTAGCCGTATTCATTG	161
卵巢组织
AMPK	上游：GCTTGATTTGGTTCCCAGGACAGT	131
	下游：GTGCTGAGTTTGCTGTACCCATGT	131
GLUT4	上游：TTACCTGGAGATCGAATTCTGCTTG	164
	下游：GTGCTTTCGATGAAGTCTCACCTG	164
PPARγ	上游：TCACGCAAGAGCAAGTAG	157
	下游：CTGGACATTAGTGGGATGAG	157
GAPDH	上游：AGAAGGCTGGGGCTCATTTG	186
	下游：AGGGGCCATCCACAGTCTTC	186

基因名称	引物序列（5′→3′）	产物大小/ bp
子宫内膜
ARA70	上游：TTGCTACTGGAACAGGGACAC	283
	下游：CCCGTGTGTTAGTTCTGCTGT	283
CBP	上游：CTCCCCAAGTTGCTGA	191
	下游：CCCCTGTAGCGGGCAAA	191
SCR1	上游：AAGTATGGGTGGATGGAGTTCAG	143
	下游：ATGAGGTGCTAAGTGTCTTTCAG	143
HOXA10	上游：CCCACCAACATACAAAGCAG	172
	下游：TGAGCATAGCAAAGGTCAGG	172
GAPDH	上游：AGGTTGTCTCCTGCGACTTCA	161
	下游：CTGTTGCTGTAGCCGTATTCATTG	161
卵巢组织
AMPK	上游：GCTTGATTTGGTTCCCAGGACAGT	131
	下游：GTGCTGAGTTTGCTGTACCCATGT	131
GLUT4	上游：TTACCTGGAGATCGAATTCTGCTTG	164
	下游：GTGCTTTCGATGAAGTCTCACCTG	164
PPARγ	上游：TCACGCAAGAGCAAGTAG	157
	下游：CTGGACATTAGTGGGATGAG	157
GAPDH	上游：AGAAGGCTGGGGCTCATTTG	186
	下游：AGGGGCCATCCACAGTCTTC	186

组别	FINS/（mU/L）	FBG/（mmol/L）	2 h PBG/（mmol/L）	ISI	IR
空白对照组	12.42±1.02	4.58±1.28	4.91±0.36	-3.16±0.08	2.59±0.59
多囊卵巢综合征组	37.66±1.54^a	14.12±1.55^a	7.93±0.75^a	-5.82±0.06^a	22.73±0.82^a
白术附子汤组	33.92±1.79^b	10.30±1.06^b	6.84±0.52^b	-5.03±0.12^b	15.52±0.76^b
八珍汤组	26.68±2.30^b	8.23±1.71^b	6.32±0.59^b	-4.47±0.18^b	9.51±0.81^b
八珍汤合白术附子汤组	16.45±1.62^bcd	5.83±1.94^bcd	5.03±0.89^bcd	-3.83±0.17^bcd	4.27±0.58^bcd
己烯雌酚组	38.94±1.52^be	13.96±1.57^be	7.82±0.72^be	-5.71±0.34^be	23.05±1.05^be
F	542.963^＊	68.781^＊	32.811^＊	419.992^＊	1 346.924^＊

组别	FINS/（mU/L）	FBG/（mmol/L）	2 h PBG/（mmol/L）	ISI	IR
空白对照组	12.42±1.02	4.58±1.28	4.91±0.36	-3.16±0.08	2.59±0.59
多囊卵巢综合征组	37.66±1.54^a	14.12±1.55^a	7.93±0.75^a	-5.82±0.06^a	22.73±0.82^a
白术附子汤组	33.92±1.79^b	10.30±1.06^b	6.84±0.52^b	-5.03±0.12^b	15.52±0.76^b
八珍汤组	26.68±2.30^b	8.23±1.71^b	6.32±0.59^b	-4.47±0.18^b	9.51±0.81^b
八珍汤合白术附子汤组	16.45±1.62^bcd	5.83±1.94^bcd	5.03±0.89^bcd	-3.83±0.17^bcd	4.27±0.58^bcd
己烯雌酚组	38.94±1.52^be	13.96±1.57^be	7.82±0.72^be	-5.71±0.34^be	23.05±1.05^be
F	542.963^＊	68.781^＊	32.811^＊	419.992^＊	1 346.924^＊

组别	E₂/（pmol/L）	T/（nmol/L）	LH/（pmol/L）	FSH/（pmol/L）	PRL/（pmol/L）	AMH/（pmol/L）	IGF-1/（pmol/L）
空白对照组	101.07±2.87	124.91±2.27	85.65±7.83	19.11±8.45	270.58±10.73	8.75±3.36	1 031.46±16.53
多囊卵巢综合征组	36.91±6.04^a	172.14±2.29^a	189.22±6.13^a	65.73±7.89^a	462.70±4.59^a	32.13±1.25^a	1 864.66±27.03^a
白术附子汤组	46.85±1.83^b	146.63±2.79^b	158.00±10.67^b	44.80±5.01^b	404.50±9.31^b	25.80±1.51^b	1 775.17±24.12^b
八珍汤组	62.35±5.27^b	139.15±2.83^b	136.19±10.15^b	37.81±6.87^b	329.25±8.51^b	22.29±3.27^b	1 630.93±10.81^b
八珍汤合白术附子汤组	81.14±6.32^bcd	132.53±1.74^bcd	101.05±6.26^bcd	28.70±4.99^bcd	291.01±4.74^bcd	17.72±1.75^bcd	1 228.33±37.36^bcd
己烯雌酚组	91.97±5.11^be	126.68±2.04^be	86.19±10.03^be	26.32±6.79^b	280.72±5.59^be	11.02±2.57^be	1 052.40±28.64^be
F	275.842^＊	554.231^＊	236.003^＊	60.253^＊	1 033.561^＊	134.461^＊	2 114.824^＊