Correlation of serum TRPV1, DNMT1 and SFRP5 with the severity of allergic rhinitis

doi:10.11958/20260009

Abstract

Abstract:

Objective To investigate the correlation between serum transient receptor potential vanilloid 1 (TRPV1), DNA methyltransferase 1 (DNMT1) and secreted frizzled related protein 5 (SFRP5) with the severity of allergic rhinitis (AR) in patients. Methods A total of 120 AR patients treated in our hospital from June 2022 to June 2025 were enrolled and categorized into the mild to moderate AR group (75 cases, TNSS total score≤8) and the severe AR group (45 cases, TNSS total score≥9) based on the total nasal symptom score (TNSS). Serum levels of TRPV1, DNMT1, SFRP5 and immunoglobulin E(IgE) were measured using enzyme-linked immunosorbent assay (ELISA) kits. Pearson correlation analysis was used to evaluate the correlation of serum TRPV1, DNMT1 and SFRP5 levels with TNSS score and serum IgE level. Multivariate Logistic regression analysis was performed to assess the impact of serum TRPV1, DNMT1 and SFRP5 levels on AR progression. Receiver operating characteristic (ROC) curve was plotted to evaluate the value of serum TRPV1, DNMT1 and SFRP5 levels alone and in combination for predicting severe AR. Results Compared with the mild to moderate AR group, the proportion of patients with concomitant asthma, TNSS scores and serum levels of IgE, TRPV1 and DNMT1 increased in the severe AR group, and serum level of SFRP5 was decreased (P<0.01). Serum levels of TRPV1 and DNMT1 were positively correlated with TNSS score and IgE (P<0.01), while the serum level SFRP5 was negatively correlated with TNSS score and IgE (P<0.01). Multivariate Logistic regression analysis showed that elevated serum TRPV1 (OR=1.844, 95%CI: 1.283-2.650) and DNMT1 (OR=2.356, 95%CI: 1.552-3.577) levels, and decreased SFRP5 (OR=0.865, 95%CI: 0.798-0.937) level were independent risk factors for progression of severe cases in AR patients (P<0.05). The AUC values of single and combined predictions of serum TRPV1, DNMT1 and SFRP5 levels for severe AR were 0.775 (95%CI: 0.690-0.846), 0.844 (95%CI: 0.766-0.904), 0.808 (95%CI: 0.727-0.875) and 0.921 (95%CI: 0.858-0.963), respectively. Compared with single indicator prediction, the combined prediction value of three indicators was higher (P<0.05). Conclusion In severe AR patients, serum TRPV1 and DNMT1 levels are elevated, while SFRP5 level is reduced. The alterations in these three biomarkers are closely associated with AR disease severity. Combined detection of the three indicators can be used for early prediction of disease progression.

Key words: rhinitis, allergic, TRPV cation channels, DNA (cytosine-5-)-methyltransferase 1, SFRP5

CLC Number:

R765.21

Figures/Tables 6

References 21

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组别	n		年龄/岁		性别（男/女）		病程/年
轻中症AR组	75		35.52±4.26		34/41		4.54±1.31
重症AR组	45		34.33±4.31		21/24		5.03±1.82
t或χ2			1.475		0.020		1.709
组别	BMI/（kg/m²）		吸烟史		合并哮喘		家族过敏史
轻中症AR组	22.83±1.25		15（20.00）		11（14.67）		28（37.33）
重症AR组	22.41±1.27		10（22.22）		19（42.22）		24（53.33）
t或χ²	1.771		0.084		11.390^＊＊		2.932
组别		接触粉尘史		IgE/（IU/mL）		TNSS评分/分
轻中症AR组		4（5.33）		235.65±68.40		5.84±1.02
重症AR组		7（15.56）		512.30±102.50		9.64±1.24
t或χ²		3.530		17.725^＊＊		18.202^＊＊

组别	n		年龄/岁		性别（男/女）		病程/年
轻中症AR组	75		35.52±4.26		34/41		4.54±1.31
重症AR组	45		34.33±4.31		21/24		5.03±1.82
t或χ2			1.475		0.020		1.709
组别	BMI/（kg/m²）		吸烟史		合并哮喘		家族过敏史
轻中症AR组	22.83±1.25		15（20.00）		11（14.67）		28（37.33）
重症AR组	22.41±1.27		10（22.22）		19（42.22）		24（53.33）
t或χ²	1.771		0.084		11.390^＊＊		2.932
组别		接触粉尘史		IgE/（IU/mL）		TNSS评分/分
轻中症AR组		4（5.33）		235.65±68.40		5.84±1.02
重症AR组		7（15.56）		512.30±102.50		9.64±1.24
t或χ²		3.530		17.725^＊＊		18.202^＊＊

组别	n	TRPV1/（μg/L）	DNMT1/（μg/L）	SFRP5/（ng/L）
轻中症AR组	75	5.93±1.25	6.25±1.36	20.78±4.63
重症AR组	45	7.14±1.07	8.09±1.28	15.11±4.41
t		5.410^＊＊	7.333^＊＊	6.610^＊＊

组别	n	TRPV1/（μg/L）	DNMT1/（μg/L）	SFRP5/（ng/L）
轻中症AR组	75	5.93±1.25	6.25±1.36	20.78±4.63
重症AR组	45	7.14±1.07	8.09±1.28	15.11±4.41
t		5.410^＊＊	7.333^＊＊	6.610^＊＊

指标	TNSS评分	IgE
TRPV1	0.479^＊＊	0.516^＊＊
DNMT1	0.506^＊＊	0.524^＊＊
SFRP5	-0.482^＊＊	-0.511^＊＊