Abstract: Objective　To evaluate and compare the diagnostic value of HPV E6/E7 mRNA test and HPV DNA test in classifying population of atypical squamous cells of undetermined significance (ASCUS) by systematic review and Meta-analysis. Methods　MEDLINE, EMbase, Cochrane Library, WanFang, Web of Science, VIP and CNKI database were electronically searched, and studies on HPV E6 /E7 mRNA test and HPV DNA test in classifying patients with ASCUS， and data were collected from January 1, 2010 to December 31, 2019. Literatures were independently screened by two reviewers. After extracting data and evaluating the risk of bias of included studies, Meta-Disc1.4 software and Stata12.1 were used for meta-analysis. Results　A total of 15 studies including 4 019 ASCUS patients were included. The pooled sensitivity, specificity, positive likelihood ratio, negative likelihood ratio and diagnostic odds ratio of HPV E6 /E7 mRNA test were 0.85 (95%CI: 0.82-0.87), 0.61 (95%CI: 0.59-0.63), 2.40 (95%CI: 1.98-2.92), 0.28 (95%CI：0.23-0.33) and 9.21 (95%CI: 6.72-12.62), respectively. Those of HPV DNA test were 0.91 (95%CI: 0.89-0.93), 0.43 (95%CI: 0.41-0.45), 1.59 (95%CI: 1.38-1.83), 0.24 (95%CI: 0.16-0.35) and 7.12 (95%CI: 4.51-11.26), respectively. The areas under the curve (AUC) of HPV E6/E7 mRNA test and HPV DNA test for detecting CINⅡ+ were 0.847 and 0.760 respectively. Conclusion　Compared with HPV DNA test, HPV E6/E7mRNA test has a higher clinical value in classifying patients with ASCUS, and it shows a high specificity in cervical lesions with CINⅡ+ in the ASCUS population.

Key words: uterine cervical neoplasms, human papillomavirus, Meta-analysis, HPV DNA test, HPV E6/E7 mRNA test, atypical squamous cells