Meta-analysis of oral microbiota changes in patients with oral squamous cell carcinoma

doi:10.11958/20252316

Abstract

Abstract:

Objective To assess changes in composition of oral microbiota in oral squamous cell carcinoma (OSCC) by systematic review and Meta-analysis. Methods Computer searches were conducted in databases such as PubMed, Embase, Cochrane Library, Web of Science, Wanfang Data and CNKI to gather research on the oral microbiome of OSCC. The search covered the period from the establishment of databases to March 25 2025. Literature was screened and data extracted according to literature inclusion and exclusion criteria, and Meta-analysis of changes in the abundance of bacterial genera in included studies was performed using Stata 17.0. Results A total of 23 studies involving 1 718 participants were included. These studies were divided into two categories: (1) case-control studies (n=14) and (2) cancer tissue versus paired paracancerous tissue (n=9). At the genus level, Fusobacterium was increased in abundance in OSCC patients (SMD=0.52，95%CI：0.39-0.65，P<0.001) and cancer tissue (SMD=0.89，95%CI：0.55-1.24，P<0.001). Porphyromonas was increased abundance in OSCC patients (SMD=0.17，95%CI：0.02-0.33，P=0.030) and cancer tissue (SMD=0.31，95%CI：0.10-0.53，P=0.005). Streptococcus was decreased in OSCC (SMD=-0.43，95%CI：-0.85--0.01，P=0.044) and cancer tissue (SMD=-0.66，95%CI：-0.96--0.37，P<0.001). Conclusion Fusobacterium and Porphyromonas increase and Streptococcus decrease in OSCC patients and cancer tissue, suggesting that oral flora dysbiosis is associated with the development of OSCC.

Key words: mouth neoplasms, carcinoma, squamous cell, microbiota, Meta-analysis

CLC Number:

R739.85

Figures/Tables 11

References 42

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文献	国家	样本类型	对照方式	样本量/例	平均年龄/岁	测序区域	OSCC中细菌属丰度变化	NOS评分
Al-hebshi等^[7]（2017）	美国	癌组织，口腔拭子	OSCC/HC	20/20	53.6±10.4/ 52.3±8.9	V1—V3	梭杆菌属↑，链球菌属↓，嗜血杆菌属↓，罗氏菌属↓	7
Takahashi等^[8]（2019）	日本	唾液	OSCC/HC	60/80	63.7/65.1	V3—V4	消化链球菌属↑，梭杆菌属↑，普雷沃氏菌属↑，二氧化碳噬纤维菌属↑，嗜血杆菌属↓，罗氏菌属↓	6
He等^[9] （2024）	中国	唾液	OSCC/HC	80/179	52.5/56.2	V3—V4	链球菌属↑，二氧化碳噬纤维菌属↑，孪生球菌属↑，普雷沃氏菌属↓，韦荣氏球菌属↓，巨球菌属↓，毛厌氧杆菌属↓	7
Ganly等^[10]（2019）	美国	口腔冲洗液	OSCC/HC	18/12	59.8±10.9/44.4±15.8	V3—V4	梭杆菌属↑，普雷沃氏菌属↑，链球菌属↓	7
Granato等^[11]（2021）	巴西	唾液	OSCC/HC	8/8	-	V4	梭杆菌属↑，卟啉单胞菌属↑，孪生球菌属↑，月形单胞菌属↑，乏养菌属↑，罗氏菌属↓，链球菌属↓，韦荣球菌属↓	6
Zhu等^[12]（2023）	中国香港	口腔冲洗液	OSCC/HC	91/91	63±11/-	V3—V4	梭杆菌属↑，消化链球菌属↑，月形单胞菌属↑，链球菌属↓，链小粒菌属↓	6
Yan等^[13]（2023）	美国	口腔拭子	OSCC/HC	31/35	66/63	-	梭杆菌属↑，消化链球菌属↑，奈瑟菌属↑，小单胞菌属↑，链球菌属↓	7
Heng等^[14]（2022）	中国	唾液	OSCC/HC	29/30	61.97±10.11/ 56.63±11.12	V3—V4	二氧化碳噬纤维菌属↑，链球菌属↓，韦荣氏球菌属↓	7
Yang等^[15]（2018）	中国台湾	口腔冲洗液	OSCC/HC	41/51	53.7±9.4/ 31.2±8.6	V3—V4	梭杆菌属↑，卟啉单胞菌属↑，奈瑟菌属↓，嗜血杆菌属↓，链球菌属↓，韦荣氏球菌属↓	6
Zhou等^[16]（2021）	中国	唾液	OSCC/HC	47/46	-	V4—V5	梭杆菌属↑，韦荣氏球菌属↑，卟啉单胞菌属↑，链球菌属↓，奈瑟菌属↓，普雷沃氏菌属↓	6
Guerrero-Preston等^[17]（2016）	美国	唾液	OSCC/HC	20/25	66/-	V3—V5	嗜血杆菌属↑，乳酸菌属↑，普雷沃氏菌属↑，链球菌属↑，韦荣氏球菌属↑	7
Haider等^[18]（2024）	巴基斯坦	口腔拭子	OSCC/HC	60/80	57.4±8.2/ 57.1±7.8	-	梭杆菌属↑，卟啉单胞菌属↑	6
Yang等^[19]（2025）	中国台湾	口腔拭子	OSCC/HC	20/15	57.14±10.44/53.00±12.99	V3—V4	梭杆菌属↑，普雷沃氏菌属↑，链球菌属↓	6
Isono等^[20]（2025）	日本	唾液	OSCC/HC	104/112	66.1±13.4/57.9±14.6	V1—V2	普雷沃氏菌属↑，卟啉单胞菌属↑，韦荣氏球菌属↓，放线菌属↓	7
Nie等^[21]（2022）	中国	癌组织	CT/PT	37/37	57.9±9.6	V3—V4	梭杆菌属↑，普雷沃氏菌属↑，卟啉单胞菌属↑，弯曲菌属↑，聚集杆菌属↑	7
Chang等^[22]（2018）	中国	癌组织	CT/PT	6/6	51.4±10.4	V3—V4	梭杆菌属↑，卟啉单胞菌属↑，链球菌属↓	6
Sarkar等^[23]（2021）	中国	癌组织	CT/PT	50/50	52.68	V3—V4	棒状杆菌属↑，奇异球菌属↑，假单胞菌属↑，普雷沃氏菌属↑，放线菌属↓，链球菌属↓，无氧芽孢杆菌属↓，沙雷氏菌属↓	6
Yang等^[24]（2021）	印度	癌组织	CT/PT	23/23	61.9±12.3	V3—V4	二氧化碳噬纤维菌属↑，卡托纳菌属↑，孪生球菌属↑	7
Zhou等^[25]（2020）	中国	癌组织	CT/PT	24/24	57.2	V3—V4	梭杆菌属↑，链球菌属↑，消化链球菌属↑，小单胞菌属↑，福赛斯坦菌属↑，肉杆菌属↑	6
Su等^[26]（2021）	中国	口腔拭子	CT/PT	74/74	53	V4	梭杆菌属↑，普雷沃氏菌属↑，消化链球菌属↑，弯曲菌属↑，二氧化碳噬纤维菌属↑，链球菌属↓	6
Zhang等^[27]（2020）	中国台湾	口腔拭子	CT/PT	50/50	60.7	V3—V4	梭杆菌属↑，卟啉单胞菌属↑，普雷沃氏菌属↑，链球菌属↓，罗氏菌属↓，韦荣氏球菌属↓	6
Ye等^[28]（2021）	中国	癌组织	CT/PT	23/23	63.0±9.6	V3—V4	放线菌属↓，链球菌属↓，奈瑟菌属↓	6
Torralba等^[29]（2021）	美国	癌组织	CT/PT	18/18	-	V4	梭杆菌属↑，消化链球菌属↑，卟啉单胞菌属↑，普雷沃氏菌属↑，小单胞菌属↑，链球菌属↓，韦荣氏球菌属↓	7

文献	国家	样本类型	对照方式	样本量/例	平均年龄/岁	测序区域	OSCC中细菌属丰度变化	NOS评分
Al-hebshi等^[7]（2017）	美国	癌组织，口腔拭子	OSCC/HC	20/20	53.6±10.4/ 52.3±8.9	V1—V3	梭杆菌属↑，链球菌属↓，嗜血杆菌属↓，罗氏菌属↓	7
Takahashi等^[8]（2019）	日本	唾液	OSCC/HC	60/80	63.7/65.1	V3—V4	消化链球菌属↑，梭杆菌属↑，普雷沃氏菌属↑，二氧化碳噬纤维菌属↑，嗜血杆菌属↓，罗氏菌属↓	6
He等^[9] （2024）	中国	唾液	OSCC/HC	80/179	52.5/56.2	V3—V4	链球菌属↑，二氧化碳噬纤维菌属↑，孪生球菌属↑，普雷沃氏菌属↓，韦荣氏球菌属↓，巨球菌属↓，毛厌氧杆菌属↓	7
Ganly等^[10]（2019）	美国	口腔冲洗液	OSCC/HC	18/12	59.8±10.9/44.4±15.8	V3—V4	梭杆菌属↑，普雷沃氏菌属↑，链球菌属↓	7
Granato等^[11]（2021）	巴西	唾液	OSCC/HC	8/8	-	V4	梭杆菌属↑，卟啉单胞菌属↑，孪生球菌属↑，月形单胞菌属↑，乏养菌属↑，罗氏菌属↓，链球菌属↓，韦荣球菌属↓	6
Zhu等^[12]（2023）	中国香港	口腔冲洗液	OSCC/HC	91/91	63±11/-	V3—V4	梭杆菌属↑，消化链球菌属↑，月形单胞菌属↑，链球菌属↓，链小粒菌属↓	6
Yan等^[13]（2023）	美国	口腔拭子	OSCC/HC	31/35	66/63	-	梭杆菌属↑，消化链球菌属↑，奈瑟菌属↑，小单胞菌属↑，链球菌属↓	7
Heng等^[14]（2022）	中国	唾液	OSCC/HC	29/30	61.97±10.11/ 56.63±11.12	V3—V4	二氧化碳噬纤维菌属↑，链球菌属↓，韦荣氏球菌属↓	7
Yang等^[15]（2018）	中国台湾	口腔冲洗液	OSCC/HC	41/51	53.7±9.4/ 31.2±8.6	V3—V4	梭杆菌属↑，卟啉单胞菌属↑，奈瑟菌属↓，嗜血杆菌属↓，链球菌属↓，韦荣氏球菌属↓	6
Zhou等^[16]（2021）	中国	唾液	OSCC/HC	47/46	-	V4—V5	梭杆菌属↑，韦荣氏球菌属↑，卟啉单胞菌属↑，链球菌属↓，奈瑟菌属↓，普雷沃氏菌属↓	6
Guerrero-Preston等^[17]（2016）	美国	唾液	OSCC/HC	20/25	66/-	V3—V5	嗜血杆菌属↑，乳酸菌属↑，普雷沃氏菌属↑，链球菌属↑，韦荣氏球菌属↑	7
Haider等^[18]（2024）	巴基斯坦	口腔拭子	OSCC/HC	60/80	57.4±8.2/ 57.1±7.8	-	梭杆菌属↑，卟啉单胞菌属↑	6
Yang等^[19]（2025）	中国台湾	口腔拭子	OSCC/HC	20/15	57.14±10.44/53.00±12.99	V3—V4	梭杆菌属↑，普雷沃氏菌属↑，链球菌属↓	6
Isono等^[20]（2025）	日本	唾液	OSCC/HC	104/112	66.1±13.4/57.9±14.6	V1—V2	普雷沃氏菌属↑，卟啉单胞菌属↑，韦荣氏球菌属↓，放线菌属↓	7
Nie等^[21]（2022）	中国	癌组织	CT/PT	37/37	57.9±9.6	V3—V4	梭杆菌属↑，普雷沃氏菌属↑，卟啉单胞菌属↑，弯曲菌属↑，聚集杆菌属↑	7
Chang等^[22]（2018）	中国	癌组织	CT/PT	6/6	51.4±10.4	V3—V4	梭杆菌属↑，卟啉单胞菌属↑，链球菌属↓	6
Sarkar等^[23]（2021）	中国	癌组织	CT/PT	50/50	52.68	V3—V4	棒状杆菌属↑，奇异球菌属↑，假单胞菌属↑，普雷沃氏菌属↑，放线菌属↓，链球菌属↓，无氧芽孢杆菌属↓，沙雷氏菌属↓	6
Yang等^[24]（2021）	印度	癌组织	CT/PT	23/23	61.9±12.3	V3—V4	二氧化碳噬纤维菌属↑，卡托纳菌属↑，孪生球菌属↑	7
Zhou等^[25]（2020）	中国	癌组织	CT/PT	24/24	57.2	V3—V4	梭杆菌属↑，链球菌属↑，消化链球菌属↑，小单胞菌属↑，福赛斯坦菌属↑，肉杆菌属↑	6
Su等^[26]（2021）	中国	口腔拭子	CT/PT	74/74	53	V4	梭杆菌属↑，普雷沃氏菌属↑，消化链球菌属↑，弯曲菌属↑，二氧化碳噬纤维菌属↑，链球菌属↓	6
Zhang等^[27]（2020）	中国台湾	口腔拭子	CT/PT	50/50	60.7	V3—V4	梭杆菌属↑，卟啉单胞菌属↑，普雷沃氏菌属↑，链球菌属↓，罗氏菌属↓，韦荣氏球菌属↓	6
Ye等^[28]（2021）	中国	癌组织	CT/PT	23/23	63.0±9.6	V3—V4	放线菌属↓，链球菌属↓，奈瑟菌属↓	6
Torralba等^[29]（2021）	美国	癌组织	CT/PT	18/18	-	V4	梭杆菌属↑，消化链球菌属↑，卟啉单胞菌属↑，普雷沃氏菌属↑，小单胞菌属↑，链球菌属↓，韦荣氏球菌属↓	7

菌属	病例对照研究		配对组织研究
菌属	t	P	t	P
梭杆菌属	1.840	0.092	0.300	0.770
卟啉单胞菌属	1.030	0.332	1.060	0.367
普雷沃氏菌属	2.040	0.072	-0.310	0.770
链球菌属	-1.500	0.161	0.350	0.735
嗜血杆菌属	0.310	0.763	0.920	0.400
奈瑟菌属	0.330	0.752	-1.480	0.214

菌属	病例对照研究		配对组织研究
菌属	t	P	t	P
梭杆菌属	1.840	0.092	0.300	0.770
卟啉单胞菌属	1.030	0.332	1.060	0.367
普雷沃氏菌属	2.040	0.072	-0.310	0.770
链球菌属	-1.500	0.161	0.350	0.735
嗜血杆菌属	0.310	0.763	0.920	0.400
奈瑟菌属	0.330	0.752	-1.480	0.214