Clinical significance of expression levels of serum Spexin and Pannexin 1 in elderly patients with diabetic cataract

doi:10.11958/20240821

Abstract

Abstract:

Objective To study the correlation between expression levels of serum Spexin and Pannexin 1 protein, glucose and lipid metabolism and insulin resistance in elderly patients with diabetic cataract.Methods A total of 118 elderly diabetic cataract patients were selected as the case group, and 103 elderly diabetic non-cataract patients were selected as the control group who were treated in the same hospital during the same period. Serum levels of Spexin and Pannexin1 were detected by enzyme-linked immunosorbent assay (ELISA). Fasting blood glucose (FPG) and glycosylated hemoglobin (HbA1c) were measured. Lipid metabolism related indicators including triacylglycerol (TG), high density lipoprotein cholesterol (HDL-C), total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) were detected in two groups of patients. Insulin resistance related indexes: fasting insulin (FINS), insulin sensitivity index (ISI), insulin resistance index (HOMA-IR) and islet beta cell function index (HOMA-β) were also detected. The correlation between serum Spexin and Pannexin1 levels, glucolipid metabolism and ISI was detected by Pearson method. Receiver operating characteristic (ROC) curves were used to assess the predictive value of serum Spexin and Pannexin1 levels and their combination in the development of cataract in elderly diabetic patients.Results The level of Spexin was lower in the observation group than that in the control group, and the level of Pannexin1 was higher than that in the control group (P<0.01). Levels of TG, TC, LDL-C, FPG, HbA1c, FINS and HOMA-IR of the observation group increased compared with the control group, while HDL-C, ISI and HOMA-β levels decreased compared with the control group (P<0.01). Pearson correlation analysis showed that serum Spexin level was positively correlated with ISI and HOMA-β, and negatively correlated with TG, TC, LDL-C, FPG, HbA1c, FINS and HOMA-IR in elderly patients with diabetic cataract (P<0.05). Serum Pannexin1 was negatively correlated with ISI and HOMA-β, and positively correlated with TG, TC, LDL-C, FPG, HbA1c, FINS and HOMA-IR (P<0.05). There was no correlation between the above indexes and HDL-C (P>0.05). The combination of both serum Spexin and Pannexin1 predicted the development of cataracts in elderly diabetic patients was better than serum Spexin and Pannexin1 assessed individually (Z _{both combination-Spexin}=3.220, P=0.001, Z _{both combination-Pannexin1}=4.838, P<0.001).Conclusion In elderly patients with diabetic cataract, the expression of serum Spexin decreases and the expression of Pannexin1 increases, and they have a certain correlation with glucose and lipid metabolism and insulin resistance

Key words: diabetes mellitus, cataract, insulin resistance, Spexin, Pannexin 1, glycolipid metabolism, aged

CLC Number:

R776.1

Figures/Tables 8

References 24

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组别		n	性别（男/女）		年龄/岁		饮酒（是/否）		吸烟（是/否）		眼底盘周厚度/μm
对照组		103	46/57		70.03±8.26		52/51		58/45		91.23±9.33
观察组		118	66/52		69.82±8.17		61/57		72/46		93.51±9.45
χ²或t			2.796		0.187		0.032		0.503		1.791
组别	糖尿病病程/年			眼压/mmHg		体质量指数/（kg/m²）		收缩压/mmHg		舒张压/mmHg		视力/D
对照组	3.16±0.61			15.39±2.03		24.59±3.46		123.92±15.14		80.75±9.17		0.73±0.09
观察组	3.28±0.57			15.62±2.17		24.13±3.43		126.37±15.76		81.06±9.64		0.71±0.07
t	1.572			0.811		0.991		1.175		0.245		1.941

组别		n	性别（男/女）		年龄/岁		饮酒（是/否）		吸烟（是/否）		眼底盘周厚度/μm
对照组		103	46/57		70.03±8.26		52/51		58/45		91.23±9.33
观察组		118	66/52		69.82±8.17		61/57		72/46		93.51±9.45
χ²或t			2.796		0.187		0.032		0.503		1.791
组别	糖尿病病程/年			眼压/mmHg		体质量指数/（kg/m²）		收缩压/mmHg		舒张压/mmHg		视力/D
对照组	3.16±0.61			15.39±2.03		24.59±3.46		123.92±15.14		80.75±9.17		0.73±0.09
观察组	3.28±0.57			15.62±2.17		24.13±3.43		126.37±15.76		81.06±9.64		0.71±0.07
t	1.572			0.811		0.991		1.175		0.245		1.941

组别	n	Spexin	Pannexin1
对照组	103	2.61±0.42	6.27±1.36
观察组	118	2.17±0.34	8.12±2.04
t		8.601^＊＊	7.811^＊＊

组别	n	Spexin	Pannexin1
对照组	103	2.61±0.42	6.27±1.36
观察组	118	2.17±0.34	8.12±2.04
t		8.601^＊＊	7.811^＊＊

组别		n	TG/（mmol/L）		HDL-C/（mmol/L）		TC/（mmol/L）
对照组		103	1.28±0.27		1.96±0.29		3.19±0.41
观察组		118	3.36±0.41		1.58±0.23		5.28±0.62
t			44.425^＊＊		10.777^＊＊		30.025^＊＊
组别	LDL-C/（mmol/L）			FPG/（mmol/L）		HbA1c/%		FINS/（mU/L）
对照组	2.17±0.24			5.61±0.69		4.26±0.57		13.86±1.57
观察组	2.65±0.29			10.22±1.64		5.53±0.66		19.05±2.16
t	13.731^＊＊			27.865^＊＊		15.231^＊＊		20.639^＊＊