Abstract: Objective: To evaluate the distributions of ganglia and cajal cells in congenital imperforate anus and to discuss clinic significance. Methods: HE staining and immunohistochemical analysis of congenital imperforate anus samples from patients with congenital imperforate anus were done for ganglia, myenteric plexuses, NSE and c-kit. Results: HE staining showed us that decreases proportion of ganglia and myenteric plexuses in the anus samples with CIA. A significantly higher proportion of myenteric plexuses was more c-kit and NSE positive expression in low-position imperforate anus than that in superior position imperforate anus (P<0.05)．Conclusion: The postoperative defacation disorders may be related to the decrease of interstitial cells of cajal and ganglia in the myenteric plexuses.

Key words: imperforate anus, congenital, anus function, ganglia, interstitial cells of cajal