Abstract:

[Abstract] Objective To study the different expressions of neurotensin (NT) at gene and protein level in orthotopic model of prostate cancer .Methods The animal models of androgen dependent prostate cancer(ADPC,castrated for3days) and androgen independent prostate cancer(AIPC) were established by planting tumor tissue or undergoing surgical castra? tion. Affymetrix microarray technology was carried out to compare the gene expressions of NT. The result was verified by qRT-PCR. HE staining was used to observe the change of pathology. ELSIA and immunohistochemistry technology were fi? nally performed to detect protein expression of prostate-specific antigen(PSA) and NT in three different groups of prostate cancer tumor tissues.Results The expression of NT was5.10times higher in AIPC group than that in ADPC group. The ex? pression of NT was0.33times lower in castrated 3-day group than that in ADPC group. Results of RT-PCR and qRT-PCR showed that the expression levels of NT gene were1.41and7.27times respectively higher in AIPC group than that in ADPC group，but the expression levels of NT gene were0.78and0.46times respectively lower in castrated 3-day group than that in ADPC group (P＜0.05). HE results showed that nuclear atypia and tumor structure in three groups. Immunohistochemistry and ELISA results showed that the values of PSA and NT were (0.48±0.03) and (0.031±0.008) μg/L in ADPC group; (0.17± 0.03) and (0.021±0.004)μg/L in castrated3-day group，and (0.87±0.02) and (0.042±0.010)μg/L in AIPC group. There were significantly lower expressions of NT and PSA in castrated3-day group that those of ADPC group (P<0.01). Conclusion In the transition from ADPC to AIPC, the over-expression of NT suggested that NT may be associated with prostate cancer progression. NT may be used as a new therapeutic target and specific diagnostic method of AIPC

Key words: androgen independent prostate cancer, NT, animal model