Abstract: Abstract Objective:To investigate the effect of schisandra chinensis on the expression of MCP-1 and iNOS in renal tissue of mice with Diabetic Nephropathy induced by streptozotocin(STZ). Methods:Twenty-four male C57BL/6 mice were randomly divided into three groups:normal control group,diabetic model group and SM-treated group.The mouse model of diabetes was induced by STZ intraperitoneally.The serum level of glucose,body weight and urinary albumin creatinine ratio(UACR) were measured. Furthermore,the morphological changes of renal tissue were observed under microscope.The expression of mRNA in kidney tissues were determined by RT-PCRl method.The protein expression in kidney tissues were determined by immunohistochemical method. Results:Compared with the diabetic model group,there were significantly lower level of serum glucose and UACR in SM-treated group,SM could also reduce the extracellular matrin accumulation in glomerular mesangial region and renal tubule and interstitial area. There were lower protein and mRNA expression of MCP-1 and iNOS in SM group.,too(P<0.05).Conclusion:After treatment of SM, the expression of MCP-1 and iNOS were decreased, which may reduce renal inflammation and relieve renal injury.

Key words: schisandraceae, diabetic nephropathies, MCP-1, iNOS, mice, C57BL