Abstract:

[Abstract]  Objective   To identify the significance of expression and phosphorylation of protein kinase R(PKR) and
nuclear factor NF-κB p65in cervical lesions, and the effect of high-risk human papilloma virus（hsHPV) on expression and phosphorylation of R(PKR) and NF-κB p65.  Methods  A total of67patients with cervical cancer,149patients with cervical intraepithelial neoplasia (CINⅠ-Ⅲ) and15normal control were included in this study. The expression levels of PKR,phosphorylated PKR (p-PKR), NF-κB p65and phosphorylated NF-κB p65(p-NF-κB p65) were detected by immunohistochemical SP method in three groups.  Results  The positive expression rates of PKR and p-PKR in cytoplasm were significantly lower in hsHPV positive group than those in hsHPV negative group (27.2% and11.0%vs41.1% and21.1%，χ2=4.858and4.371，P＜0.05). The positive expression rates of NF-κB p65and p-NF-κB p65in cytoplasm and nucleus were significantly higher in hsHPV positive group than those in hsHPV negative group (46.3% ,25.7% ,22.8% and12.5%vs32.6%,14.7%,11.6% and4.2%,χ2=4.345,4.048,4.729and 4.650 respectively，P＜0.05). The positive expression rates of PKR in kytoplasm and karyon were significantly lower in NF-κB p65(+) group than those in NF-κB p65(-) group (25.5%vs38.0% and20.4%vs36.3%，χ2=3.898and4.396respectively, P＜0.05). The positive expression rate of PKR in kyto?plasm was significantly lower in p-NF-κB p65(+) group than those in p-NF-κB p65(-) group (19.0%vs36.0%,χ2=4.462，P＜0.05).  Conclusion  hsHPV may inhibit the expression and phosphorylation of PKR but promote the expression andphosphorylation of NF-κB p65. The expression and phosphorylation of NF-κB p65may inhibit the expression of PKR. Regulating effects of three may be associated with the generation and progression of cervical cancer.

Key words: uterine cervical neoplasms, cervical intraepithelial neoplasia, protein kinases, eIF-2kinase, phosphory-lation, NF-kappa B