Abstract: Abstract Objective： To construct the recombinant plasmid containing the double luciferase report gene and the HL promoter -514/-250 sites.and tansfect the plasmids to HepG2 cells.Then to analyse HL transcriptional activity. Method: Construct luciferase reporter vector containing HL promoter -514T/-250A and -514C/-250G polymorphisms, tested by Dual Luciferase Reporter Assay System, use Fluoroskan Ascent FL test the 10 second value. Result: The luciferase reporter vector contain with HL promoter -514T+-250A present higher HL transcriptional activity than the vector with -514C+-250G. Conclusion: HL promoter -514C together with -250G alleles are associated with higher HL tanscriptional activity, so proved that HL promoter polymorphisms can influence the HL transcriptional activity on the molecular mechanism.

Key words: Hepatic lipase, Promoter, Polymorphism, Transcriptional activity, Luciferase