Abstract: Objective: To explore the expression and significance of Cyclin D1, Cyclin A, Cyclin B1 and PHH3 in human glioma, and study the correlation between parameters and clinicopathological features. Methods: Expression of Cyclin D1, Cyclin A, Cyclin B1 and PHH3 were analyzed by S-P immunohistochemical analysis in 59 cases of gliomas（gradeⅠ ten cases, grade Ⅱ fifteen cases, grade Ⅲ fifteen cases, grade Ⅳ nineteen cases） and 37 normal brain tissues. Results: Expression of Cyclin D1, Cyclin A, Cyclin B1 and PHH3 were not found in normal brain tissues. The positive expression rate of Cyclin D1, Cyclin A, Cyclin B1 and PHH3 in gliomas were 69.5%、69.5%、62.7% and 84.7% respectively. With the increasing of pathological grades, the mean labeling indexes for Cyclin D1、Cyclin A、Cyclin B1 and PHH3 in human gliomas increased accordingly. The difference of Cyclin D1、Cyclin A、Cyclin B1、PHH3 labeling index between four groups were significant（P<0.01）. Conclusion: Cell cycle phase can be analyzed by immunohistochemical stain, and it is beneficial for tumor grading and helpful to predict the prognosis.

Key words: glioma, Cyclin D, Cyclin A, Cyclin B1, PHH3, immunohistochemistry