Abstract: Objective To explore the effect of over-expression of miRNA-495 combined with temozolomide (TMZ) on
the proliferation and apoptosis in the glioma cells and the related mechanisms. Methods MiRNA-495 mimics and negative
control sequences (miRNA-495 NC) were transfected into U251 cells and A172 cells by LipofectamineTM 2000 in glioma.
The relative expression levels of miRNA-495 and HMGN5 mRNA were detected by quantitative real-time PCR (qPCR). The
protein expression levels of HMGN5 and MMP-9 were detected by Western blot assay. The effects of transfection on the
proliferation of U251 and A172 cells were detected by CCK-8. After the action of miRNA-495 mimics and TMZ alone or in
combination on U251 cells, the apoptosis rate and proliferation inhibition rate of U251 cells were detected by flow cytometry
and CCK-8 assay. Results In the glioma U251 and A172 cells, the relative expression of miRNA-495 was increased in the
miRNA-495 mimics group compared with that of the blank control group and the miRNA-495 NC group. The relative
expressions of HMGN5 mRNA, HMGN5 and MMP-9 protein were decreased in the miRNA-495 mimics group compared with those of the blank control group and the miRNA-495 NC group. The ability of cell proliferation was significantly
decreased (P＜0.05). Compared with the TMZ group and the miRNA-495-mimics group, the cell proliferation in U251 cells
was significantly inhibited, and the apoptosis was significantly decreased in TMZ+miRNA-495 mimics group. Conclusion
The over expression of miRNA-495 and in combination with TMZ can co-inhibit the proliferation and promote apoptosis of
glioma cells, which may be related with the inhibition of HMGN5/MMP-9 signaling pathway.

Key words: glioma, HMGN proteins, matrix metalloproteinase 9, cell proliferation, apoptosis, miRNA-495, HMGN5,
temozolomide