Abstract: nPKC and cPKC Modulate Translocation of Glucose Transporter 4 in Skeletal Muscle Cells LI Jinru，NIU Wenyan，CHU Guilan Basic Mdical College, Tianjin Medical University,Tianjin 300070, China Abstract Objective: To evaluate the effects of novel protein kinase C (nPKC) and conventional protein kinase C(cPKC) on modulation of translocation of glucose transporter 4 (GLUT4) in skeletal muscle cells. Methods: To determinate the level of GLUT4myc translocation on cellular surface by treatment with PKC activator PMA and PKC inhibitors Go6976, Go6983 in C2C12GLUT4myc myotubes. Results: Acute stimulation of PMA can significantly increase the translocation of GLUT4myc, Go6976, Go6983 inhibited the translocation of GLUT4myc in different degrees. Chronic stimulation of PMA partly inhibited the translocation of GLUT4myc on cellular surface. Conclusion: Activation of nPKC or cPKC enhance translocation of GLUT4 in Skeletal muscle cells.

Key words: nPKC, cPKC, Glucose transporter 4, Translocation