Tianjin Medical Journal

Tianjin Med J ›› 2016, Vol. 44 ›› Issue (2): 155-158.doi: 10.11958/58919

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The effect of miRNA-7 on chemoresistance in esophageal cancer cell TE-1

WEN Shuang1， YANG Xiaoyu2， ZHANG Min1， CHU Xiufeng1， ZHONG Genshen3， JI Yinghua1△， LU Ping1△   

  1. 1 Department of Oncology, the First Affiliated Hospital of Xinxiang Medical University, Henan 453100, China;2 Department of Pathology, Xinxiang Medical University; 3 Research Institution of Neurology, the First Affiliated Hospital of Xinxiang Medical College
  • Received:2015-05-11 Revised:2015-09-19 Published:2016-02-15 Online:2016-02-15
  • Contact: △Corresponding Author E-mail:sunny8441_cn@sina.com；lupingdoctor@126.com E-mail:wenshuang5288@163.com

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Abstract: Objective To explore the impacts of over-expression of microRNA-7 (miRNA-7) on the sensitivity of cisplatin in esophageal carcinoma cell line TE-1, and the possible mechanism thereof. Methods Lipofectmin 2000 method was used to transient transfect with miRNA-7 mimic into esophageal cancer cell line TE-1, which was taken as transfection group, mimic negative control was taken as transfection conrtol group. The expressions of miRNA- 7 and epidermal growth factor receptor (EGFR) mRNA were detected by RT-PCR in the above two groups and normal control group. The total EGFR and EGFR in cytoplasmic and nucleus were detected with Western blot assay in transfection group and transfection control group. CCK-8 was used to detect IC50 of cisplatin in transfection group and transfection control group. The expression of EGFR was observed with immunofluorescence confocal microscope in two groups. Results The miRNA-7 expression was significantly increased in transfection group than that of transfection conrtol group and control group. The expression of EGFR mRNA was significantly reduced in transfection group (P＜0.001). The total EGFR was significantly decreased in transfection group than that of transfection conrtol group. The level of nuclear EGFR was significantly increased (P＜0.01)， and cytoplasm EGFR expression was significantly decreased in transfection group than that of transfection control group (P＜0.05). CCK-8 results showed that after the over expression of miRNA-7 in TE-1, the IC50 of cisplatin (48 h) increased in transfection group than that of control group (P＜0.01). Immunofluorescence results showed that EGFG in nuclear was higher in transfection group than that of transfection control group but its expressions reduced in cell membrane and cytoplasm. Conclusion The over-expressed miRNA-7 in esophageal cancer cells TE-1 can reduce cisplatin sensitivity by the increased EGFR in nuclear translocation.

Key words: esophageal neoplasms, cisplatin, receptor, epidermal growth factor, drug resistance, neoplasm, microRNAs, nuclear translocation, miRNA-7