Abstract: Abstract Objective: To investigate the relationship between alcohol and Pulmonary fibrosis and possible mechanism by observing the effect of chronic alcohol intake on lung tissues in rats and tissue inhibitor of metalloproteinase-1 glutathione. Methods: Clean healthy male Sprague-Dawley rats 20, weighing 160-200g, were randomly dividedinto control group (C group) 10 and alcohol group (A group) 10, and fed quantitative non-ethanol or ethanol Lieber-DeCarli liquid diet every day in a single cage for 16 weeks. By Masson staining collagen deposition were observed in alveolar interval ; t ere detected.Results: ⑴Masson results show: the alcohol group had more deposition of collagenfibers at intervals of alveolar than the control group.⑵Compared to the group C, the content of GSH in rat lung tissue reduced but HYP increased in group A ,the differences were statistically significant ( P <0.05).⑶TIMP-1 in rat lung tissue of group A were higher than group C, the differences were statistically significant(P <0.05). Conclusion: Chronic alcohol ingestion can cause collagen deposition in alveolar interval and higher expression of TIMP-1 in lung tissue . Alcohol may be one of the reasons for pulmonary fibrosis in rats.

Key words: alcohol, pulmonary fibrosis, tissue inhibitor of metalloproteinase-1 glutathione