Abstract:

[Abstract] Objective To investigate the effects of doxycycline on the expressions of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metaloproteinase-1(TIMP-1) in breast cancer lung metastasis model mice (BCML-TAⅡ 99), and the mechanism thereof. Methods The BCML-TAⅡ99mouse model was established. The immunohistochemical staining was performed to detect the expressions of MMP-9and TIMP-1in BCML- TAⅡ99model mice and controls． Results The metastasis rate was significantly lower in treatment group than that of control group. The expression of MMP-9 was significantly reduced, but the expression of TIMP-1was significantly increased, in treatment group compared with those of control group (P<0.05). Conclusion Doxycycline can inhibit tumor growth and metastasis in breast cancer with1ung metastasis model mice by regulating MMP-9and TIMP-1expressions．

Key words: Doxycycline, Breast cancer, Matrix metalloproteinase9 (MMP-9), Tissue inhibitor of metalloproteinase1(TIMP-1), mice, inbred strains