Abstract: Objective To explore the effect of 1,25-dihydroxyvitamin D3 on insulin resistance and inflammatory response in mice with severe burns under stress. Methods 130 mice were randomly divided into: the healthy group (n = 10), the experimental group I (n = 40), the experimental group II (n = 40), the control group (n = 40). Each mouse was scalded in the experimental group I ,the experimental group II and control group to 30% of the total body surface area (TBSA) III °. Mice in experimental group I received 1，25-(0H)2VitD3 1μg?kg-1 with 0.6 mL peanut oil by gavaging at 8 o'clock every other day.At the same time and by the same way, mice in experimental group II received 1，25-(0H)2VitD3 4μg?kg-1 with 0.6 mL peanut oil，and mice in control group received only 0.6mL peanut oil. FBG, FIns, serum TNF-α concentrations and wound tissue NF-κB positive rate were determined respectively on day 1, day3, day7 and day 14 after burn . Results (1) At each time point of the experimental groupⅠ, the experimental groupⅡand control group, the means of HOMA-IR , Serum TNF-α concentrations and wound tissue NF-κB positive rate were higher than those in healthy group. (2) The means of HOMA-IR of scalded mice in experimental group Ⅰand experimental groupⅡ were lower than those in control group at the same time, and the means of experimental group Ⅱ were lower than those in the experimental group Ⅰ (P <0.05); at the different time points ,the means of HOMA-IR of scalded mice in same group were the highest at day 3, and then gradually decreased (P <0.05). (3) The means of Serum TNF-α concentrations and wound tissue NF-κB positive rate of scalded mice in experimental group Ⅰand experimental groupⅡ were lower than those in control group at the same time, and the means of experimental group Ⅱ were lower than those in the experimental group Ⅰ (P <0.05); at the different time points ,the means of Serum TNF-α concentrations and wound tissue NF-κB positive rate of scalded mice in same group were gradually decreased (P <0.05). Conclusion 1,25-dihydroxyvitamin D3 can reduce insulin resistance and inflammatory response in mice with severe burns under stress.

Key words: 25-dihydroxyvitamin D3, mice, burns, insulin resistance, tumor necrosis factor-alpha, nuclear factor-kappa B