Abstract: Objective: To observe the effect of valsartan, atorvastatin and pioglitazone on the expression of TGF-β1, extracellular matrix, adrenomedullin and complement factor H in mesangial cells cultured under high glucose levels and to investigate its mechanism. Methods: The experiment was divided into low glucose group(LG), high glucose group (HG), high glucose+valsartan(HD), high glucose+atorvastatin (HL)and high glucose+pioglitazone(HP) . ELISA and RIA were used to measure the content of TGF-β1，laminin and type Ⅳ collagenin of the cultured mesangial cells under high glucose conditions. RT-PCR was applied to determine the expression of TGF-β1mRNA, AMmRNA and CfhmRNA. Results: Valsartan, atorvastatin and pioglitazone could significantly decrease the concentrations of TGF-β1，laminin and type Ⅳ collagen in the supernatants for a certain extent compared with HG control group（P<0.01）,and they could also inhibit the expression of TGF-β1mRNA, AMmRNA and CfhmRNA of the cultured mesangial cells under high glucose conditions（P<0.01）. Conclusions: Valsartan, atorvastatin and pioglitazone showed a certain renoprotective functions which might relates to the inhibition of over-expression of TGF-β1 and extracellular matrix in mesangial cells induced by high glucose. Mean while, the expression of AM and Cfh was reduced, which might also relates to the variation of TGF-β1.

Key words: diabetic nephropathies, mesangial cells, valsartan, pyrroles, thiazolidinediones, transforming growth factor beta1, adrenomedullin, 补体因子H