Abstract: Abstract Objective:To investigate the immune regulatory function of total flavones of Epimedium on immunosuppression mice. Methods:The immunosuppression model was established by using cyclophosphamide(CY). The body weight and spleen weight were analysed. The absolute number of total splenocytes and bone marrow cells were determined by haemacytometer.The absolute number of CD4+T cells ,CD8+ T cells,B cells and NK cells in spleen was detected by flow cytometer. Results:Total Flavones of Epimedium significantly antagonist the loss in immune organ and body weight induced by cyclophosphamide, markedly restore the number of total splenocytes and bone marrow cells , improved the number of CD4+T cells ,CD8+ T cells,B+ cells and NK cells in spleen . Conclusion:Total Flavones of Epimedium could improve the immune function in immunodepressed mice induced by CY.

Key words: Total flavons of epimedium, Cyclophosphamide, Immunomodulation