Abstract: 【Abstract】 Abstract Objective:To investigate the biological effects on hepatoma cell Hep3B of p62 by use of p62 siRNA. Methods: First,Three siRNA sequences specifically targeting p62 (named as Si-h-GTF2H1_001, Si-h-GTF2H1_002 and Si-h-GTF2H1_003) were selected,the best one which silent p62 was Si-h-GTF2H1_002.The siRNAs were transfected into hepatoma cell Hep3B with Lipofectamine 2000.Then,the exprement were classified four groups: 1,blank control group；2,siRNA group；3,Ncontrol（NC）group；4,Lipofectamine（Lip）group.The expression of p62,xpd,p53,cyclinD1 were detected by RT-PCR and Western blot.The cell cycle were examined by MTT.Results : Contrast with blank control group , NC group, Lip group ,the expression of p62 mRNA was decreased significantly in siRNA group(p＜0.01）;the expression of xpd mRNA and p53 mRNA were decreased obviously;but the expression of cyclinD1 mRNA was enhanced obviously.The trends of protein was consistent with mRNA.MTT results showed that cell proliferation increased in siRNA group. Conclusion: p62 gene may initeract with xpd,p53 and cyclinD1 to inhibit cancer cell,and possibly regulate cell proliferation through DNA damage checkpoint.

Key words: hepatoma cell, small interference RNAs, gene, p62, transfection, cell cycle