Abstract: Objective   To investigate the proliferation, immune phenotype and cytotoxicity on different cell lines of CIK cells collected from healthy donors. Methods   Peripheral blood mononuclear cell(PBMC) from healthy donors were induced to become CIK cells by adding cytokines including rhIL-2, rhIFN-γ and CD3 McAb. Proliferation of CIK cells was tested by blood cell recording board. The CIK cells were analyzed on different time points by FACS; The cytotoxicity of CIK cells against different tumor cell lines such as K562, BJAB, A549, MCF-7, HepG2 in vitro by MTT assays on day 13. Results  CIK cells quickly proliferated from day 5, and expanded by 182-fold after 20 days’ culture. The immunophenotypes of CD3+, CD3+CD8+, CD3+CD56+ were (97.83±1.03) %, (77.12±1.60) % and (27.58±2.02) %. The percentages of CD3+, CD3+CD8+, CD3+CD56+ increased noticeably (P<0.01). According to the effector-target ratio of 40:1, the activity of CIK cells against tumor cells K562, BJAB, A549, MCF-7, HepG2 were (88.89±7.22) %, (75.42±9.52) %, (63.19±5.67) %, (43.53±5.67) % and (42.63±7.69) %. The experiment showed that CIK cells possessed higher antitumor cytotoxic activity. Conclusion   CIK cells can be largely capacity cultured by adding cytokines in vitro. CIK cells was a highly efficient cytotoxic cells against tumors, and had clinical application potentials.

Key words: Immunophenotyping, cells, cultured, cell line, tumor, leukemia, lymphoma, 肺肿瘤