Abstract: [Abstract] Objective To observe the effect of autophagy inhibitor on the activation of alcohol induced hepatic stel? late cells, and the mechanisms thereof. Methods HSC-T6cells were cultured in vitro and divided into four groups, includ? ing blank control group, alcohol group, 5mmol/L3-MA+alcohol group (low alcohol group) and10mmol/L3-MA+alcohol group (high alcohol group). RT-PCR was used to detect the expression levels ofα-smooth muscle actin (α-SMA) and typeⅠ collagen. The levels of LC3Ⅱ, α-SMA and typeⅠcollagen were detected by Western blot assay. The cell viability of HSC T6was detected by MTT assay.Results The mRNA expressions ofα-SMA, typeⅠcollagen and the protein of expressions α-SMA, typeⅠcollagen and LC3Ⅱwere significantly up-regulated in alcohol group compared with those of control group (P<0.05), while the expressions of those parameters were significantly down- regulated in 10mmol/L3-MA+alcohol group (P<0.01). The mRNA and protein levels of α-SMA and typeⅠcollagen were significantly decreased in two3-MA-treated groups compared with those in alcohol group (P<0.05). Meanwhile, compared with the 5mmol/L3-MA+ alcohol group，the protein expressions ofα-SMA, typeⅠcollagen and LC3Ⅱwere significantly decreased in10mmol/L3-MA+alcohol group (P<0.05). Compared with the alcohol group，there was significantly lower proliferation activity in all two 3-MA-treated groups (P<0.05). Conclusion 3-MA can inhibit the protein expression of LC3Ⅱ, α-SMA and typeⅠcollagen induced by alcohol in HSC-T6cells, and inhibit the proliferation of HSC cells.

Key words: autophagy, alcohol, 3-methyladenine, hepatic stellate cells