Abstract:

[Abstract]   Objective  To investigate the effect of compound catechu anti- diarrhea ointment on the expression of
aquaporin3(AQP3) in the colonic tissues of diarrhea rat model, and the mechanism thereof.   Methods   Forty SD rats were randomly divided into four groups: blank control group, model control group, positive control group and compound catechu anti-diarrhea ointment group. Rats were given6g blank matrix cream in model control group,2mL suspension of berberine in positive control group and compound catechu anti-diarrhea ointment in compound catechu anti-diarrhea ointment group, two times/d for 7d. The rat model of diarrhea was established by using senna intragastric administration. The water content of feces was measured. The expression of AQP3in colonic tissues was detected by immunohistochemistry assay.   Results   The water contents of feces were significantly higher in model control group (64.09±0.41) %than those of other three groups(F=53.879，P＜0.05). There was no significant difference in the water content of feces between compound catechu anti-diarrhea ointment group (48.83±1.08)% and positive control group (46.87±2.19)%. The AQP3-positive cells were mainly expressed in the intestinal mucosa. The dyeing index was significantly lower in model control group (0.85±0.18) than that of other three groups (F=14.971，P＜0.05). There was no significant difference in the dyeing index between compound catechu anti-diarrhea ointment group (1.30±0.18) and positive control group (1.37±0.14).    Conclusion   Compound catechu anti-diarrhea ointment can significantly reduce the water contents of feces, which may be related to the increased AQP3expression in colonic tissues.

Key words: aquaporin 3, compound catechu anti- diarrhea ointment, diarrhea, colon, water metabolism