Abstract:

[Abstract]???Objective???To investigate the expression of epidermal growth factor-like domain7(EGFL-7) in brain tis?
sue of diabetes mellitus (DM) rats, and the intervene effect of amioguanidine and baicalein thereof.??Methods???Forty-eight SD rats were randomly divided into following four groups: control group, diabetic group, aminoguanidine group and baicalein group (n=12for each group). The control group received only pelleted food and water. Diabetic group, aminoguanidine group and baicalein group were given high fat and sugar diet in combination with small dose of intraperitoneal injection of streptozotocin to establish the diabetic model. And then aminoguanidine group and baicalein group were given aminoguanidine and baicalein150mg/(kg·d) through oral gavaging. Diabetic group and control group were given equivalent saline through oral gavaging. After16-week treatment, the expression of EGFL-7proteins in rat brain was detected by immunohistochemistry and Western blotting methods.??Results???Results of immunohistochemistry and Western blotting assay showed that the expression of EGFL-7was significantly increased in diabetic group, aminoguanidine group and baicalein group compared with that of control group (P<0.01). When compared with diabetic group, the expression of EGFL-7was significantly decreased in aminoguanidine group and baicalein group (P<0.01). There was no significant difference in the EGFL-7expression between aminoguanidine group and baicalein group (P>0.05).???Conclusion????EGFL-7may play an important role in cerebral vascular hyperplasia and remodeling of diabetic rats. Aminoguanidine and baicalein restrained cerebral vessel growth by reducing the expression of EGFL-7.

Key words: diabetes mellitus, type2, EGFL-7, amioguanidine, baicalein, vascular hyperplsia, vascular remodeling