Effects of quercetin on periodontal tissue angiogenesis during orthodontic tooth movement in rats by regulating HIF-1α/VEGF signaling pathway

doi:10.11958/20240663

Abstract

Abstract:

Objective To investigate the impacts of quercetin (QUE) on angiogenesis and hypoxia inducible factor-1α/vascular endothelial growth factor (HIF-1α/VEGF) signaling pathway in periodontal tissue of orthodontic tooth movement in rats. Methods A rat model of orthodontic tooth movement was constructed, and the modeled rats were randomly separated into the model group, the low-dose quercetin treatment (QUE-L) group, the high-dose quercetin treatment (QUE-H) group and the QUE-H+pathway inhibitor YC-1 (QUE-H+YC-1) group, with 12 rats in each group. Another 12 rats were regarded as the control group. The movement distance of the first molar of rats in each group was measured. HE staining was applied to observe the pathological changes of periodontal tissue and count the number of blood vessels generated in periodontal tissue. Immunohistochemistry was applied to detect expression levels of bone morphogenetic protein 2 (BMP2) and bone morphogenetic protein 4 (BMP4) in periodontal tissue. Western blot assay was applied to detect the expression of HIF-1α/VEGF signaling pathway related proteins. Results Compared with the control group, the periodontal tissue fibers were scattered and disordered in the model group, with an increased intercellular space, infiltration of a large number of inflammatory cells and bone resorption pits, the movement distance of the first molar increased, the number of periodontal tissue blood vessels decreased, and the expression of BMP2, BMP4, HIF-1α and VEGF decreased (P<0.05). The fibrous arrangement of periodontal tissue was relatively tight in the QUE-L group and the QUE-H group compared to the model group, with reduced infiltration of inflammatory cells and reduced alveolar bone resorption. The movement distance of the first molar decreased, the number of periodontal tissue blood vessels increased, and the expression of BMP2, BMP4, HIF-1α and VEGF increased (P<0.05). Compared with the QUE-H group, the periodontal tissue fibers in the QUE-H+YC-1 group were scattered and disordered, with obvious intercellular gaps, increased infiltration of inflammatory cells and increased bone resorption pits, the movement distance of the first molar increased, the number of blood vessels in periodontal tissue decreased, and the expression of BMP2, BMP4, HIF-1α and VEGF decreased (P<0.05). Conclusion Quercetin can promote angiogenesis in periodontal tissue during orthodontic tooth movement in rats, and its mechanism is related to the activation of the HIF-1α/VEGF signaling pathway.

Key words: quercetin, hypoxia-inducible factor 1, vascular endothelial growth factors, orthodontic extrusion, periodontium, vascular remodeling

CLC Number:

R783.5

Figures/Tables 6

References 22

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组别	第一磨牙移动距离/mm^#	血管数/支^##
Control组	0.00±0.00	3.36±0.22
Model组	0.58±0.06^a	0.75±0.08^a
QUE-L组	0.41±0.04^b	1.81±0.14^b
QUE-H组	0.25±0.03^bc	3.25±0.19^bc
QUE-H+YC-1组	0.49±0.05^d	1.53±0.12^d
F	363.698^＊＊	616.525^＊＊

组别	第一磨牙移动距离/mm^#	血管数/支^##
Control组	0.00±0.00	3.36±0.22
Model组	0.58±0.06^a	0.75±0.08^a
QUE-L组	0.41±0.04^b	1.81±0.14^b
QUE-H组	0.25±0.03^bc	3.25±0.19^bc
QUE-H+YC-1组	0.49±0.05^d	1.53±0.12^d
F	363.698^＊＊	616.525^＊＊

组别	HIF-1α	VEGF
Control组	0.92±0.07	1.21±0.11
Model组	0.25±0.03^a	0.33±0.03^a
QUE-L组	0.43±0.04^b	0.62±0.05^b
QUE-H组	0.74±0.06^bc	0.97±0.07^bc
QUE-H+YC-1组	0.35±0.04^d	0.76±0.05^d
F	188.500^＊＊	147.210^＊＊

组别	HIF-1α	VEGF
Control组	0.92±0.07	1.21±0.11
Model组	0.25±0.03^a	0.33±0.03^a
QUE-L组	0.43±0.04^b	0.62±0.05^b
QUE-H组	0.74±0.06^bc	0.97±0.07^bc
QUE-H+YC-1组	0.35±0.04^d	0.76±0.05^d
F	188.500^＊＊	147.210^＊＊

组别	BMP2	BMP4
Control组	0.81±0.08	0.87±0.09
Model组	0.31±0.03^a	0.35±0.04^a
QUE-L组	0.52±0.05^b	0.57±0.06^b
QUE-H组	0.77±0.08^bc	0.85±0.09^bc
QUE-H+YC-1组	0.46±0.05^d	0.49±0.05^d
F	72.080^＊＊	65.121^＊＊