Abstract:

[Abstract]   Objective   To investigate the effectiveness of bone marrow mesenchymal stem cell (MSC) and different
transplantation methods of MSC on adriamycin (ADR) model of nephropathy in rats.   Methods   The ADR model of nephropathy was induced by left nephrectomy plus injection of ADR (2.5mg/kg) in Sprague-Dawley (SD) rats, once a week for two weeks. The model rats with nephropathy were randomly divided into three groups: adriamycin nephropathic model control group (ADR,n=12), MSCs transplantation through right renal artery group (M-A, n=12) and MSCs transplantation through peripheral veins group (M- V,n=12). Another 12SD rats were served as normal controls (N,n=12). MSCs were cultured,transplanted via right renal artery (2×10⁶/mL) to rats in M-A group, and were transplanted via peripheral veins 2×10⁶/mL)to rats in M-V group. The same procedure was repeated in two weeks. The blood urea nitrogen, serum creatinine, 24h urine protein and24h uromicroprotein were detected before transplantation and in one and two weeks after the second transplantation. The renal morphology and labeled cells were examined in the kidney one week after the second transplantation.  Results   The values of blood urea nitrogen, serum creatinine,24h urine protein and24h uromicroprotein were significantly higher in M-A group, M-V group and ADR group than those of N group (P＜0.01). The level of 24h uromicroprotein was significantly lower before the second transplantation in M-A group than that of ADR group (P＜0.01). The serum level of creatinine was significantly decreased in M- A group than that of ADR group and M- V group (P＜0.01). The levels of 24h urine protein and24h uromicroprotein were significantly lower after one week transplantation in M-A group than those of MV group (P＜0.01). The serum level of creatinine was significantly lower two weeks after the second transplantation in M-A
group than that of ADR group and M-V group (P＜0.01), but no significant differences in the levels of urine protein and uromicroprotein between M- A group and M- V group.  Conclusion   Transplantation of MSCs can alleviate renal damage ofchronic ADR-induced nephropathy, which is more effective in rats with MSCs transplantation via renal artery than that in rats with MSCs transplantation via peripheral vein.

Key words: nephrosis, mesenchymal stem cells, bone marrow transplantation, mesenchymal stem cell transplantation, disease models, animal, rats, Sprague-Dawley