Effects of Morin on neuronal apoptosis in cerebral ischemia-reperfusion rats by inhibiting TXNIP/NLRP3/Caspase-1 signaling pathway

doi:10.11958/20221258

Abstract

Abstract:

Objective To investigate the influence of Morin on neuronal apoptosis in cerebral ischemia-reperfusion rats by regulating TXNIP/NLRP3/Caspase-1 signaling pathway. Methods Sixty rats were randomly grouped into the sham operation group, the model group, the Morin low-dose group (Morin-L, 10 mg/kg), the Morin high-dose group (Morin-H, 40 mg/kg), the Morin-H+pLVX-NC group (40 mg/kg Morin+pLVX-NC) and Morin-H+pLVX-TXNIPgroup (pLVX-TXNIP, 40 mg/kg Morin+pLVX-TXNIP). The rat middle cerebral artery embolism model was established by the suture method (except for the sham group), and then perfused after 1 h of administration. After 72 hours of perfusion, neurological deficits were scored. The whole brain was removed for brain edema determination. Hematoxylin-Eosin (HE) staining was performed to observe the pathological damage in the penumbra area of brain tissue. TTC was performed to detect the infarct size of brain tissue. ELISA was performed to detect serum contents of IL-1β and IL-18. Western blot assay was used to detect expression levels of TXNIP/NLRP3/Caspase-1 signaling pathway-related proteins in brain tissue. Results Compared with the sham operation group, the neurological deficit score, contents of IL-1β and IL-18, brain water content, infarct size, expression levels of TXNIP, NLRP3 and Caspase-1 proteins were increased in the model group (P<0.05). Compared with the model group, the neurological deficit score, contents of IL-1β and IL-18, brain water content, infarct size, expression levels of TXNIP, NLRP3 and Caspase-1 proteins were decreased in the Morin-L group and the Morin-H group (P<0.05). Compared with Morin-H+pLVX-NC group, the neurological deficit score, contents of IL-1β and IL-18, brain water content, infarct size, expression levels of TXNIP, NLRP3 and Caspase-1 proteins were increased in the Morin-H+pLVX-TXNIP group (P<0.05). Conclusion Morin can reduce brain injury and inhibit neuronal apoptosis in rats with cerebral ischemia-reperfusion, which may be related to the inhibition of TXNIP/NLRP3/Caspase-1 signaling pathway.

Key words: reperfusion injury, hypoxia-ischemia, brain, disease models, animal, Samlutein, TXNIP/NLRP3/Caspase-1 signaling pathway, apoptosis of neuron

CLC Number:

R543.4

Figures/Tables 6

References 18

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组别	IL-1β	IL-18
假手术组	10.27±1.03	75.15±7.52
模型组	29.12±2.92^a	138.52±13.86^a
Morin-L组	22.04±2.21^b	106.37±10.64^b
Morin-H组	15.34±1.54^bc	84.15±8.42^bc
Morin-H+pLVX-NC组	15.27±1.53	84.28±8.43
Morin-H+pLVX-TXNIP组	24.35±2.44^de	115.68±11.57^de
F	115.929^＊＊	54.256^＊＊

组别	IL-1β	IL-18
假手术组	10.27±1.03	75.15±7.52
模型组	29.12±2.92^a	138.52±13.86^a
Morin-L组	22.04±2.21^b	106.37±10.64^b
Morin-H组	15.34±1.54^bc	84.15±8.42^bc
Morin-H+pLVX-NC组	15.27±1.53	84.28±8.43
Morin-H+pLVX-TXNIP组	24.35±2.44^de	115.68±11.57^de
F	115.929^＊＊	54.256^＊＊

组别	梗死面积（%）	脑含水量（%）
假手术组	0.00±0.00	65.12±3.01
模型组	21.05±2.11^a	85.34±3.55^a
Morin-L组	13.21±1.33^b	75.66±2.76^b
Morin-H组	8.51±0.86^bc	67.43±2.34^bc
Morin-H+pLVX-NC组	8.48±0.85	68.32±2.28
Morin-H+pLVX-TXNIP组	15.64±1.57^de	82.54±2.88^de
F	92.531^＊＊	26.751^＊＊

组别	梗死面积（%）	脑含水量（%）
假手术组	0.00±0.00	65.12±3.01
模型组	21.05±2.11^a	85.34±3.55^a
Morin-L组	13.21±1.33^b	75.66±2.76^b
Morin-H组	8.51±0.86^bc	67.43±2.34^bc
Morin-H+pLVX-NC组	8.48±0.85	68.32±2.28
Morin-H+pLVX-TXNIP组	15.64±1.57^de	82.54±2.88^de
F	92.531^＊＊	26.751^＊＊

组别	TXNIP	NLRP3	Caspase-1
假手术组	0.25±0.03	0.17±0.02	0.34±0.04
模型组	1.19±0.12^a	0.85±0.09^a	1.12±0.12^a
Morin-L组	0.63±0.07^b	0.54±0.06^b	0.77±0.08^b
Morin-H组	0.34±0.04^bc	0.24±0.03^bc	0.39±0.04^bc
Morin-H+pLVX-NC组	0.37±0.04	0.25±0.03	0.37±0.04
Morin-H+pLVX-TXNIP组	0.71±0.08^de	0.66±0.07^de	0.74±0.08^de
F	96.979^＊＊	95.663^＊＊	72.103^＊＊