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15 March 2010, Volume 38 Issue 3 Previous Issue    Next Issue
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Whole Body 18F-FDG PET/CT Imaging for Detection of Primary Tumors in Cervical Metastatic Adenocarcinoma of Unknown Primary Site ZHENG Jing,硕 高 2010, 38 (3):  0-0 .  Abstract ( 1191 )   PDF (1265KB) ( 1852 )   Abstract Objective: To retrospectively evaluate whole body 18F-FDG PET/CT in depicting the primary tomors in cervical metastatic adenocarcinoma of unknown primary site. Methods: Seventeen patients were included and underwent whole body PET/CT imaging. The primary tumors and metastases were detected by visual evaluation and measurements of standardized uptake values.The PET/CT impression was verified by histopathology and/or follow-up. Results: In 17 patienis，primary tumors of 13 patients were confirmed by pathological or clinical follow-up. Among them，8 were in the lung, 1 in breast, 1 in renal, 2 in colon and 1 in cervix. Among the other 4 cases, PET/CT correctly diagnosed 3 cases diagnosed metastatic adenocarcinoma previously but demonstrated as malignant lymphoma finally. The sensitivity, specificity and accuracy of PET/CT in identifying primary tumors were 92.3%, 75.0%, 88.2%, respectively. After PET/CT examination, 10 patients (58.8%) were detected additional metastatic lesions which were missed previously. Conclusion: Whole body 18F-FDG PET/CT may be of relatively higher value in identifying the primary tumor in cervical metastatic adenocarcinoma of unknown primary site. It also may display tumor involvement extent. Related Articles | Metrics

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analysis of 653 patients with verrucosa gastritis 2010, 38 (3):  0-0 .  Abstract ( 734 )   PDF (1291KB) ( 1959 )   Related Articles | Metrics

实验研究

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Influence of Mitochondrial Membrane Potential and Superoxide Production in FRTL Cell Induced by Hydrogen Peroxide 2010, 38 (3):  0-0 .  Abstract ( 1329 )   PDF (1739KB) ( 1856 )   Abstract Objective：To investigate the effects of exogenous hydrogen peroxide (H2O2) expose on mitochondrial superoxide production and mitochondrial membrane potential（Δψ）changes in Fisher rat thyroid cell line (FRTL) cells. Methods：Following exposed in 1 mmol/L H2O2 for 10 min, 30 min and 24 h respectively mitochondrial superoxide production in FRTL cells were measured by living cell imaging and flow cytometry using MitoSOX. After exposed in 1 mmol/L H2O2 for 30 min and 24h mitochondrial membrane potential (Δψ) was assayed by spectrofluorimeter and fluorescent microscopy using rhodamine 123(rh123). Cell viability was detected by MTT colorimetric method. Morphological changes were observed by invert microscope. Apoptosis assay was performed by acridine orange staining. Results：Quantitative measurements of the mean intensities of MitoSOX from the samples demonstrated significantly increased with 1mmol/L H2O2 following 10 min,30 min and 24 h treatment. Fluorescence intensity of rh123 in FRTL cells and optical density of MTT colorimetric method markedly decreased with 1mmol/L H2O2 following 30 min and 24 h treatment（p＜0.01）. Under light microscope and fluorescence microscope after acridine orange staining, the characteristic morphological features of programmed cell death, picknosis, karyorrhexis, and cell shrinkage were observed. Conclusion：Acute and chronic exogenous H2O2 expose cause oxide stress in FRTL cells which result in mitochondrial superoxide production increase , Δψ decline, cell necrosis and apoptosis . Related Articles | Metrics

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Expression of CD95 and OX40L in hepatocellular carcinoma 2010, 38 (3):  0-0 .  Abstract ( 1299 )   PDF (799KB) ( 1782 )   Aim: To assess the effects of the expression of CD95 and OX40 ligand (OX40L) messenger RNA (mRNA) in hepatocellular carcinoma (HCC), and to explore the clinical values. Methods: CD95 expression by CD3 positive T cells was quantitatively measured by dual color flow cytometry. Expression of OX40L mRNA were detected by TaqMan real-time reverse transcriptase- polymerase chain reaction (RT-PCR). Results: CD95 expression by CD3 positive T cells was significantly higher in patients with HCC (34±20%) compared with normal controls (20±7%) (P<0.05). The expression level of OX40L mRNA was significantly decreased in patients with HCC compared with normal controls (P<0.05). Conclusion: These results suggest that abnormal expressions of CD95 and OX40L in patients with HCC play an oncogenic role at stage of human hepatocarcinogenesis. Related Articles | Metrics

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The cerebral white matter in the Alzheimer’s disease in diffusion weighted imaging study 2010, 38 (3):  0-0 .  Abstract ( 1662 )   PDF (922KB) ( 1812 )   Objective To elucidate the changes in apparent diffusion coefficients (ADC) by quantify diffusion weighted (DW)magnetic resonance imaging (MRI)in the Alzheimer’s disease(AD). Methods DW MRI was performed in 30 probable AD patients and 30-normal controls with normal –appearing white matter (NAWM) was studied using 1.5 T MR system . apparent diffusion coefficients (ADC) were measured in different WM areas. Subjects were examined by neurologic and neuropsychological assessment with mini-mental state examination(MMSE).ADC were determined in standard regions of the frontal ，temporal ，occipital and parietal white matter ,genu , splenium of the corpus callosum . Results on DWI, there was no significant difference in the values of ADC between male and female, or between the right and left hemisphere (all p>0.05),the valuea of AD :frontal(ADC：t=-0.050，p<0.01)、parietal (ADC：t=-0.057，p<0.01)、genu of the corpus callosum (ADC：t=-0.012,p>O.05)、splenium (ADC：t=-0.028，p<0.05)、temporal(ADC：t=-0.062，p<0.01)、occipital(ADC：t=0.000，p>0.05). AD patients , ADC of splenium of the corpus callosum , parietal，frontal white matter, correlated significantly with MMSE scores. Especially parietal. Conclusion Quantitative DWI analysis may be helpful in assessing MRI DWI can detect WM abnormalities in AD ．The parietal WM abnormalities may play an important role in the development of dementia．The combination of function and structure of MRI, indicating that Alzheimer's cognitive decline and white matter closely related concept of micro-structure. Related Articles | Metrics

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Serum Cholinesterase in Patients with Sepsis and its Clinical Significance 2010, 38 (3):  0-0 .  Abstract ( 1324 )   PDF (1250KB) ( 2088 )   Abstract: Objective To explore the changes of serum cholinesterase (ChE) in patients with sepsis and its clinical significance. Methods Estimate serum cholinesterase concentration and acute physiology chronic health evaluation (APACHE) Ⅱ scores in patients. Divide the patients into the sepsis group and the non-sepsis one. Divide the patients with sepsis into the death group and the survival one. Understand the changes of serum ChE levels between the groups and the impact on assessment and prognosis. Results (1) Serum ChE in patients with sepsis group was significantly lower than the control group (P <0.01). (2) In patients with sepsis, the serum ChE levels in the death group were significantly lower compared with the survival one (P <0.05). (3) The serum ChE levels in patients with sepsis were significantly negatively correlated with APACHE Ⅱ scores (r=-0.280, P <0.05), positively correlated with serum albumin and body mass index（r = 0.458 and 0.287，P＜0.05）. Conclusion Serum ChE levels can be used to determine the severity of illness and prognosis in patients with sepsis. Related Articles | Metrics

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The Predictive Value of the Change in Pleural Pressure on the Success Rate of Pleurodesis 2010, 38 (3):  0-0 .  Abstract ( 1415 )   PDF (991KB) ( 1839 )   Abstract Objective: To explore the prediction value of the change in pleural pressure on the success rate of pleurodesis.Methods: The research is single centre and open experimental designed.We selected patients with malignant pleural effusion.Under ultrasonographic guidance, an appropriate intercostal space was selected.Then the patients were conducted the thin tube d?rainage in the pleural cavity. The pleural pressure, defined as the mean pleural pressure, was measured immediately and after 1000mL of effusion was withdrawn. Measurements were done while patients were in a sitting position. The elastance of the pleural space was calculated as the change in pleural pressure (in cm H2O) divided by the amount of fluid removed.After the effusion was completely drained off,the chest CT and HRCT on the lesion was undergone to confirm the degree and range of the involved lungs,visceral and parietal pleura. Cisplatin was instilled into the pleural cavity.After clamping the tube for 4 hours,the pleural space was drained consecutively.The tube was removed when less than 20ml of effusion was drained per day for 3 consecutive days and no effusion or only little amount of fluid was shown on the ultrasound.The intervention was scored as “successful”if no fluid reaccumulation was noted at 4 weeks, otherwise,scored as “failure”. Result:The study contained 72 patients with malignant pleural effusion.The outcome of 54 patients was successful,the other 18 patients was failure. Pleural elastance of the “successful” patients was less then the “failure”(P<0.05).All of the patients whose pleural elastance was ≥15cmH2O/L have “failure” outcome.The success rate of pleurodesis was 86.10% on the patients whose pleural elastance was <15cmH2O/L.There was no statistically difference on the success rate of pleurodesis between no obvious changes and the significant changes on images(P>0.05).Conclusion:There was good predictive value of the pleural elastance on the success rate of pleurodesis. Related Articles | Metrics