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15 April 2010, Volume 38 Issue 4 Previous Issue    Next Issue
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短篇论著
实验研究
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综述与讲座
实验研究
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实验研究
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短篇论著


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Comparative Study on Prevalence of Noso Shangwei Wu 2010, 38 (4):  0-0 .  Abstract ( 935 )   PDF (544KB) ( 2019 )   Related Articles | Metrics

实验研究

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The isolation and identification of cancer stem/progenitor cells from the ascites of ovarian cancer Patients 2010, 38 (4):  0-0 .  Abstract ( 1822 )   PDF (1250KB) ( 2119 )   The isolation and identification of cancer stem/progenitor cells from the ascites of ovarian cancer Patients WANG Cuihuan, GAO Yan, HE Xiaoying, DU Yongrui, YAO Zhi, DENG Weiming△Department of immunology, Tianjin Medical University, Tianjin 300070, China ? Abstract Objective: To isolate and identify the stem/progenitor cells from the ascites of ovarian cancer patients. Methods: To isolate ovarian cancer stem/progenitor cells by sphere-forming in serum-free culture medium and to identify the stemness of this kind of cell from following aspects: side population were detected by the flow-cytometry; to determine the expression of genes and proteins of the stem/progenitor cell-relevant markers ABCG2, Oct-4, Nanog in ascetic spheroid cells and ascetic cells by PCR and Western blot respectively; to evaluate the tumor-formation ability in the NOD/SCID mice. Results: The ascitic spheroid cells had unique growth characteristics, and SP cells accounted for 0.9% and 0.2% in ascitic spheroid cells and ascitic cells respectively. The ascitic spheroid cells expressed higher stem/progenitor cell-relevant markers ABCG2, Oct-4 and Nanog than ascitic cells; Furthermore，xenograft tumor formed only by 1×104 ascitic spheroid cells in NOD/SCID mice. Conclusions: The ovarian cancer stem/progenitor cells can be isolated by sphere-forming in serum-free culture medium from ascites and can be used as a potential tool for studying the pathogenesis and scanning the anti-cancer drugs in the treatment of human ovarian cancer. Key words ovarian cancer tumor stem/ progenitor cells isolation identification Related Articles | Metrics

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Auto-tissue engineering bone for rabbit segmental bone defects 2010, 38 (4):  0-0 .  Abstract ( 1332 )   PDF (1493KB) ( 2066 )   Objective: Implantation of auto-tissue engineering bone to cure the rabbit segmental bone defects. Methods:Culture the rabbit mesenchymal cell， prepare the auto-tissue engineering bone with CS-Gel/HA－rhBMP-2. Bone mesenchymal stem cells as seed cells and carry out complex CS-Gel/HA-rhBMP-2.The establishment of animal models of bone defect is to amputate forelimb mid-7mm length of the rabbit bone tissue . In experimental group（right side），treat the rabbit segmental bone defect model with CS-Gel/HA－rhBMP-2－seed cell,in control group (left side),the implantation is CS-Gel/HA.Not do internal and external fixation,sew up the wound layer by layer.Evaluate the grade of X-ray and histology after treatment 4,8,12 weeks.Results:There are no significant differences of X-ray grade and histology grade between experiment group and control group at 4 weeks after treatment, but the X-ray grade and histology grade is better in experiment group than in control group at 8,12weeks after treatment，data are statistically significant (p<0.05).Conclusion: The auto-tissue engineering bone can cure the rabbit segmental bone defects. Related Articles | Metrics

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Value of ultrasonography and color doppler imaging in the clinical diagnosis of orbital vascular disorders 2010, 38 (4):  0-0 .  Abstract ( 818 )   PDF (1064KB) ( 1915 )   Objective To evaluate the image characteristics of the ultrasonography(US) and color doppler imaging(CDI) in the diagnosis of orbital vascular disorders. Methods A retrospective series of 118 patients with orbital vascular disorders were involved and the image characteristics of ultrasonography and color doppler imaging were analysed. The tissue specimens of tumor were obtained during the operation for histopathological examination. Results Among 118 cases of orbital vascular disorders, 69 cases of cavernous hemangioma, 27 cases of lymphangioma, 13 cases of venou hemangioma, 5 cases of varix and 4 cases of capillary hemangioma。 In all patients, different disorders have different echograms and characteristic bloods, so it is helpful for diagnosis of there disorders through manifestation of characteristic bloods in combination with special echograms. Conclusion Both US and CDI are useful in the diagnosis of orbital vascular disorders. Related Articles | Metrics

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Clinical research of Preemptive analgesia with different dosage tramadol in pediatric anesthesia with sevoflurane 2010, 38 (4):  0-0 .  Abstract ( 1823 )   PDF (1046KB) ( 1971 )   【Abstract】objective To investigate the influence of preemptive analgesia with different dosage tramadol in children with inhalational anesthetic sevoflurane on postoperative palinesthesia time, extubation time, circulation, postoperative agitation and conscious-sedation extent. Methods Ninty children(ASAⅠgrade)undertaking appendectomy were randomly divided into low dose tramadol group(group T1，n=30) , large dose tramadol group(group T2，n=30) and control group(group C，n=30).Anaesthesia was induced with sevoflurane,fentanyl and atracurium for tracheal intubation. In group T1 and group T2, 1mg/kg and 2mg/kg tramadol was administered intravenously after intubation, and in group C, normal saline was administered intravenously, anaesthesia was maintained with sevoflurane. Extubation was accomplished after the children awaked. Results There was no significant difference of palinesthesia time, extubation time and pulse oxygen saturation among three groups(P>0．05).Compared with group C,the heart rate（HR）and mean arterial pressure（MAP）of extubating and ten miniutes after extubating in group T2 decreased significantly, the incidence of postoperative agitation in group T2 also decreased significantly,and conscious-sedation extent increased significantly(P<0．01)，the HR,MAP and the incidence of postoperative agitation in group T1 decreased slightly,but there were no statistic difference（P>0．05）。Conclusion 2mg/kg tramadol could prevent postoperative agitation effectively in school children receiving inhalational sevoflurane,obtained satisfactory conscious-sedation extent and stable circulation when extubating and after extubating,and also,there were no influence on postoperative palinesthesia time and extubating time. Related Articles | Metrics

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Chemosensitization, immunosensitization and metastatic inhibition of malignant cancer cells by nuclear factor-kappa B inhibitor 2010, 38 (4):  0-0 .  Abstract ( 1135 )   PDF (1522KB) ( 1962 )   Related Articles | Metrics

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Regulation Factors of Primordial Follicle’s Initial Recruitment 2010, 38 (4):  0-0 .  Abstract ( 1180 )   PDF (2085KB) ( 1945 )   This article summarizes the regulation factors of primordial follicle’s initial recruitment, and emphasises on the effects of ovarian local regulation factors, with the aim to provide the theoretical basis for the mechanism of follicle’s initiation. Related Articles | Metrics

实验研究

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The Pilot Study On The Influence Of The Local Tumor Microenvironments On LPPCN Formation 2010, 38 (4):  0-0 .  Abstract ( 1187 )   PDF (1206KB) ( 1962 )   Abstract: Objective: To study the influence of the local tumor microenvironments on LPPCN formation(linearly patterned programmed cell necrosis, LPPCN). Methods: The left femoral arteries of 9 C57BL mice were ligated and then B16F10 melanoma cells suspension was injected into the left ischemic skeletal muscles and non- ischemic right controls respectively to build the ischemic animal model; Biomechanical animal model was built by injecting the B16F10 melanoma cells suspension into the the abdominal cavity and left skeletal muscles of 10 C57BL mice.HE staining was performed to compare the LPPCN distribution among groups.Immunohistochemistry was employed to detect the expression of matrix metalloproteinase-2 & 9 .Results: The LPPCN cell numbers in the ischemic group were significantly higher than those in the control group （P<0.01）； and the skeletal muscle group significantly higher than those in the abdominal cavity group（P<0.01）.Similarly,the expression of MMP-2 and MMP-9 was higher in the ischemic group than that in the control group （P<0.01）and the skeletal muscle group significantly higher than those in the abdominal cavity group（P<0.01）.Conclusion:Hypoxia and microenvironment pressure of tumor can increase MMP-2, MMP-9 expression and influence the LPPCN formation. Related Articles | Metrics

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The Expression and Clinical Significance of CD147 and MMP-2 in Cervical Cancer 2010, 38 (4):  0-0 .  Abstract ( 1070 )   PDF (1150KB) ( 1904 )   The Expression and Clinical Significance of CD147 and MMP-2 in Cervical Cancer Xu Ya-hui, Wang De-hua Tianjin Medical Unervisity General Hospital. [Abstract] Objective : To study The Expression and Clinical Significanceof CD147 and MMP-2 in Cervical Cancer. Methods Using immunohistochemical SP method to detec t and analysis the expression of CD147 and MMP-2 in 20 cases of normal cervical tissue，21 cases of CIN tissue，59 cases of cervical cancer .Results 1.CD147 and MMP-2 were expressed mainly in cancerous lesions，but also expressed in some normal tissues. The expression of CD147 and MMP-2 in cancer group was significantly higher than the normal cervical group and CIN group(P<0.05). Positive expression rate of them were 91.5%和80.0%.2 Expression of CD147 in cervical cancer were related with lymphatic matastasis(χ2=4.138，P<0.05), not related with age，stage，gross type，the degree of differentiation，histological types (P> 0.05). Expression of MMP-2 were related with stage(χ2=9.423，P<0.05) and lymphatic matastasis (χ2=5.019，P<0.05), not related with age，gross type，the degree of differentiation，histological types(P> 0.05). 3. The relationship between CD147 and MMP-2 was positively correlated，and the correlation coefficient rs = 0.283 (P<0.05). Conclusion CD147 and MMP-2 plays an important role in cervical cancer invasion and metastasis，Combined detection of CD147 and MMP-2 may be a new target of cancer diagnosis to assess the severity of cervical cancer. Related Articles | Metrics

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Effects of Sevoflurane and Isoflurane on postoperative cognitive function in elderly patients undergoing off-pump Coronary Artery Bypass Grafting 2010, 38 (4):  0-0 .  Abstract ( 1354 )   PDF (1182KB) ( 1884 )   【Abstract】 Objective：To discuss the feasible anesthesia method in elderly patients undergoing off-pump Coronary Artery Bypass Grafting(OPCABG) through compare the postoperative cognitive function between Sevoflurane and Isoflurane inhaled anesthesia. Methods：Sixty undergoing elective OPCABG patients aged 65~82,heart function Ⅱ or Ⅲ ,were randomly divided into two groups: Sevoflurane group(S,n=30) and Isoflurane group(I,n=30).The blood samples of internal jugular vein were taken for determination of serum S-100 protein,neuronspecific enolase(NSE) content at the moment after anesthesia induction(T0),the end of operation(T1),6 h after operation(T2) and 24 h after operation(T3).A mini-mental state examination(MMSE) was performed 1 d before and 3,7 d after operation by an experienced psychometrician.The total scores of MMSE was 30 points.Those scores not reach 24 points were defined as postoperative cognitive dysfunction(POCD). Results：There was no significant difference in general conditions between both groups(P>0.05).All patients completed MMSE.The incidence of POCD within 7 d after operation in group I(20%) was higher than that in group S(13%)(P<0.05).The scores of MMSE at 3,7 d after operation were lower than that at 1 d before operation in both groups(P>0.05).The contents of serum S-100 protein and NSE increased at the initial stages of operation,reached peak at T1(P<0.05),then decreased at T2 (P<0.05),and returned to baseline level at T3 in both groups(P>0.05).The contents of serum S-100 protein and NSE were lower in group S than those in group I at T1 and T2(P<0.05). Conclusion： Sevoflurane can cause slighter cerebral injury and lower incidence of POCD than Isoflurane in elderly patients undergoing OPCABG,which is a recommendable anesthesia method that could be used for prevent cognitive dysfunction after heart operation. Related Articles | Metrics

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The histopathologic observation for diabetic retinopathy membrane after use anti-VEGF gene 2010, 38 (4):  0-0 .  Abstract ( 1276 )   PDF (1342KB) ( 2278 )   Purpose: To analyze the histopathologic change of proliferative membrane in patient with diabetic retinopathy after intravitreal injection of Avastin.Methods: The patients with diabetic retinopathy between January 2008 and June were divided into 2 groups randomly. Control groups recieved vitrectomy directly, but expermental groups recieved vitrectomy 10 days after intravitreal injection of Avastin（Avastin 1.25mg/0.05ml）. During surgery the proliferative membrane were peeled, and by HE staining method to observe the change of its new vessels, vascular endothelial cell, initial cell. Results: The diabetic retinopathy proliferative membrane are made up of extracellular matrix,different morphology cell and new vessels under light microscope. The difference of new vessels ,vascular endothelial cell, initial cell was statistical significant between the two groups (P<0.05). Conclusions: Intravitreal anti-VEGF gene is associated with a rapid regression of retinal neovascularization secondary to diabetic retinopathy,reduce hemorrhage and improve surgery success rates,it is an important adjunctive treatment in the management of diabetic retinopathy. Related Articles | Metrics

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The antitumor effect of SEA in sarcoma 180 and the distribution of the cytokine 2010, 38 (4):  0-0 .  Abstract ( 1251 )   PDF (1403KB) ( 2028 )   Abstract:Ojective To study the antitumor effect of SEA and the distribution of the cytokine IFN-γ in vivo,and also to investigate the mechanism of killing cancer cells. Methods: Established the tumor xenograft model with S180 cells,and three groups were injected with SEA intraperitoneally (n=10),the fourth group was injected with normal saline intraperitoneally(n=10), measured the tumor volume,drawed growth curve and calculated inhibition ratio, detected the distribution of IFN-γ from different levels.Results:The inhibition ratio of the SEA high-concentration group is 44.00%,and the content of IFN-γ increased both in tissue and in serum(*p<0.05),There is also an obvious difference between the tumor weight and the tumor voume of SEA high-concentration group and the normal saline group. But the tumor weight and the tumor volume of SEA-high concentration group decreased obviously(*p<0.05). Conclusion:SEA has an obvious antitumor effect in S180 tumor, and SEA secrets a mass of cytokine IFN-γ to inhibit sarcoma180 cells. Related Articles | Metrics

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Factors affecting human blastocyst formation in vitro , , 2010, 38 (4):  0-0 .  Abstract ( 1664 )   PDF (435KB) ( 2010 )   Factors affecting human blastocyst formation in vitro LUZhenYu ZHANG Yun Shan Miruoran Tianjin Central Hospital of Gynecology & Obsterics, Tianjin 300052, China Abstract Objective: To investigate the factors affecting human blastocyst formation in vitro. Methods: Through sequential culturing the D3 discarded embryos to D5-7, we observed the blastocyst formation and detect the relationship between the D3 embryo cells number, quality grade and the blastosysts quality, the formation of the high-quality blastocysts, providing the academic basis for the blastocyst transplantation. 406 D3 discarded embryos entered this experiment. Cultured these embryos with CCM, observed theirs changes daily till D5-7. Results: 102 blastocysts were formed including 28 high-quality blastocysts using the blastocyst grade standard from Gardener. The blastulation rate was 25.12%. Among 64 patients who have blastocysts, 32 patients pregnancied, the pregnancy rate is higher than whose without blastocysts (50%Vs28.79%, P<0.05). Conclusion: The blastultions rate is positively correlative with the D3 embryo cells numbers. The blastulation rate of the embryos withⅠ-Ⅱ+ degree is high than those with Ⅲ-Ⅳ degree. Related Articles | Metrics

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The Female Genital Tract Malignant Tumor in Adolescent--39 cases report 2010, 38 (4):  0-0 .  Abstract ( 1372 )   PDF (975KB) ( 1984 )   Abstract Objective: To analyse the character of symptom, treatment, and prognosis of female genital tract cancer in adolescent. Method: Retrospect 39 cases of adolescent female genital tract cancer treated in the Tianjin Central Hospital of Obstetrics and Gynecology from Jan. 1997 to Oct. 2008. Outcome: There were 26 ovarian cancer, which included 20 cases of germ cell tumor, 4 cases of epithelial cancer, a case of juvenile granulosa cell tumor, and a case of malignant lymphoma . Among 6 cases of uterine malignant tumor, there were 3 cases of endometrial cancer, 1 case of adenosarcoma, low grade endometrial stromal sacoma, and cervical rhabdomyosarcoma, respectively. Furthermore, there were 6 cases of gestational trophoblastic tumour and 1 case of malignant pelvic fibroma. The common symptoms were increasing of abdominal circumference and abdominal pain in ovarian cancer and passing vagina bleeding in uterine cancer. In ovarian cancer, 96.2%(25/26) patients received conservative surgery and 1 patient received radical surgery. All of the patients received adjuvant chemotherapy. In uterine cancer, 4 cases received ovarian conservative surgery, 1 case received radical surgery, and surgery wasn’t done in the cervical rhadomyosarcoma for advanced stage. Chemotherapy was done in all of the uterine cancer cases. 5 cases of the 6 GTN received combination chemotherapy of 5-FU and ACT-D. The other case of GTN wasn’t treated with chemotherapy, because she missed the treatment opportunity. The malignant pelvic fibroma patient received the debulking surgery and adjuvant chemotherapy. Average duration of follow-up period was 59.7 months. 5-years survival rate was 86.0%. All of the patients with conversation operation in early stage epithelial ovarian cancer and with ovarian preservation surgery in stageⅠendometrial cancer were survival without tumor.92.9% of patients with conservative treatment got regular menses at the sixth month post chemotherapy. Conclusion: The morbidity of adolescent female genital tract cancer is low, just engages 1.12% in all age group. Most of them are ovarian germ cell tumor. Main treatment should be conservative surgery followed with chemotherapy, which is benefit to the patients’ quality of life. Conservative surgeries can be done in stageⅠepithelial ovarian cancer and ovarian preservation surgery can be done in stageⅠendometrial cancer. There isn’t obvious influence on ovarian function post chemotherapy. Related Articles | Metrics