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15 June 2017, Volume 45 Issue 6 Previous Issue    Next Issue
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Cell and Molecular Biology
Experimental Study
Clinical Study

Diagnostic Techniques
Epidemiological Investigation
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Cell and Molecular Biology

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SND1 protein co-localization with TIA-1 on stress granules under stress stimuli SHAO Jie, ZHANG Bing-bing, ZHAO Meng, ZHOU Yun-li, REN Li 2017, 45 (6):  561-565.  doi: 10.11958/20170286 Abstract ( 1010 )   PDF (1255KB) ( 3701 )   Objective To analyze the association of staphylococcal nuclease domain-containing protein 1(SND1) and T-cell intracellular antigen 1(TIA-1) on stress granules, and the regulation of SND1 on stress granules under stress stimuli. Methods The immunofluorescence assay and laser scanning confocal microscopy were used to observe the co-localization of SND1 protein and TIA-1 protein under stress stimuli, and the over-expression plasmids of pEGFP vector were transfected into HeLa cells and to verify which domain of SND1 co- localized with TIA- 1 under stress stimuli. RNA interferencemediated knockdown of the expression of SND1 protein in HeLa cells was measured by Western Blotting assay. Then whether the knockdown of SND1 affected the recruitment of TIA- 1 on stress granules was observed. Heat shocks under different times were used to identify whether there were dynamic changes in transportation of SND1 and TIA-1 on stress granules. Results SND1 co-localized with TIA- 1 on stress granules under stress stimuli, and the associated domain of SND1 were SN domain. TIA-1 still can be recruited on stress granules but a large amount of stress granules were reduced even though the expression of SND1 protein was decreased. And the transportation of SND1 on stress granules was laged behind TIA-1 under different-times of heat shocks. Conclusion SND1 protein co-localizes with TIA-1 on stress granules, and which co-regulates the cellular stress response under stress stimuli. Related Articles | Metrics

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The effect of PKC ζ inhibitor on the proliferation and invasion of skin squamous carcinoma cell line A-431 XING Yan-ling, WU Jing 2017, 45 (6):  566-570.  doi: 10.11958/20170369 Abstract ( 987 )   PDF (815KB) ( 3510 )   Objective To investigate the effect of protein kinase C (PKC)ζ inhibitor T5450996 on the proliferation and invasion of skin squamous carcinoma cell line A-431. Methods PKCζ inhibitor T5450996 was screened through Z′-LYTE™ kit. Cell proliferation assay and cell cycle analysis were used to observe the effects of T5450996 on the proliferation of skin squamous carcinoma A-431 cells. Scratch assay and invasion assay were used to explore the effects of T5450996 on the migration and invasion of skin squamous carcinoma A-431 cells. Results The PKCζ inhibitor T5450996 can inhibit the activity of PKCζ kinase, and the IC50 value of T5450996 was about 35 μmol/L. Compared to the control group, 35 μmol/L and 70 μmol/L concentrations of T5450996 significantly suppressed the proliferation of A-431 cells and blocked the cell cycle of A-431 cells. The results of scratch assay and invasion assay indicated that the migration and invasion capacities of A-431 cells were markedly impaired after the treatments with 35 μmol/L and 70 μmol/L concentrations of T5450996 (P＜ 0.05). However, 20 μmol/L concentration of T5450996 showed no significant effect (P＞0.05). Conclusion PKCζ inhibitor T5450996 significantly inhibits the proliferation and invasion capacities of skin squamous carcinoma cell line A- 431, and which may be a small molecular inhibitor with potential applications in the future. Related Articles | Metrics

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The response of cyclic tensile strain on the BMSCs co-cultured human degenerative anulus fibrosus cells LI Shuang1, SUN Xiao-lei1, MA Xin-long1△, ZHANG Yang2, DENG Shu-cai1, HAO Yong-hong1 2017, 45 (6):  571-576.  doi: 10.11958/20161109 Abstract ( 837 )   PDF (796KB) ( 3575 )   Objective To investigate the effects of different cyclic tensile strains on the proliferation and expression of bone marrow stromal cells (BMSCs)- cocultured human degenerated anulus fibrosus (AF) cells. Methods AF cells were isolated from a patient with degenerated intervertebral disc degeneration (IVD), which were co-cultured with BMSCs. The solely cultured AF cells were used as control group. The two groups of cells were expanded in monolayer, and cyclically strained for 3 hours, which were applied 0, 5%, 10%, 15% and 20% strains at a frequency of 0.25 Hz using BioDynamic test instrument. A flow cytometry method was used to examine the AF cell proliferation at 24 hours followed the application of cyclic tensile strains. After the total RNA was extracted, real-time PCR technology was used to detect the gene expression of collagen Ⅰ and aggrecan. Results Under the same appropriate stress, the proliferative index (PI), the proportion of cells in the period of DNA synthesis, the expression of collagen Ⅰ and aggrecan were significantly higher in the co-cultured group than those of control group (P＜0.05). However, the best mechanical stimulation was different in the two groups. For the AF cells, the peaks of PI, the proportion of cells in the period of S period, the expression of collagen Ⅰ and aggrecan were found in the 10% strain group, while for the co- cultured cells, they were found in the 15% strain group. Conclusion Coculturing with BMSCs has a positive effect on the proliferation and expression of human degenerative fibrous ring cells, which can protect AF cells from bad stress stimulation. Related Articles | Metrics

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The effect of high-level of glucose on ghrelin expression in human umbilical vein endothelial cells LI Guang-kuo, ZHAO Wen, LIU Yong 2017, 45 (6):  576-579.  doi: 10.11958/20170146 Abstract ( 905 )   PDF (527KB) ( 3963 )   Objective To investigate the influence of high- level of glucose on the expression of ghrelin in human umbilical vein endothelial cells (HUVECs). Methods After 12 h glucose free culturing, the effects of different concentrations of glucose and different incubation times on expressions of ghrelin were observed in HUVECs. (1) The cells were treated with 0, 5, 10, 15, 20 mmol/L glucose for 2 h, then ghrelin mRNA expression levels were detected by RT-PCR, and the protein levels of ghrelin were detected by ELISA. (2) The cells were treated with 10 mmol/L glucose for 0, 0.5, 1, 2, 6, 12 hours, ghrelin mRNA and protein levels were detected respectively. Results (1) The expression levels of ghrelin mRNA and protein decreased along with increased glucose concentrations, which showed no obvious changes when the glucose was above 15 mmol/L. (2) The expression levels of ghrelin mRNA and protein decreased with the prolonged incubation time. But more than 6 h culturing time showed no further effect on reducing the expression level of ghrelin. Conclusion High levels of glucose can inhibit the expression level of ghrelin, which may be one of the mechanisms of atherosclerosis. Related Articles | Metrics

Experimental Study

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Protective effects of 3,3′-diindolylmethane on radiation damage of hematopoietic system in mice DONG Jia-li, LU Lu, FAN Sai-jun△ 2017, 45 (6):  580-583.  doi: 10.11958/20170457 Abstract ( 936 )   PDF (337KB) ( 3929 )   Objective To investigate the protective effect of 3,3′-diindolylmethane (DIM) on radiation-induced injury in mouse hematopoietic system. Methods Thirty C57BL/6 mice were randomly divided into control group, 2 Gy irradiation group and 2 Gy irradiation + DIM group (n=10 for each group). Mice of control group received sham irradiation, and the other two groups accepted 2 Gy 137Cs γ-ray total body irradiation. Mice in 2 Gy irradiation +DIM group were intraperitoneally injected 75 mg/kg DIM 30 min before irradiation. Mice of other two groups were treated with reference solution. After 7 d and 15 d of 2 Gy irradiation, the peripheral blood samples were collected to count the number of bone marrow nuclear cells (BMNCs). The level of reactive oxygen species(ROS) was measured by DCFH- DA. The levels of colony forming unitsgranulocyte-macrophage (CFU-GM) were also detected. Results The numbers of white blood cell (WBC), platelet count (PLT), BMNCs and CFU-GM were significantly decreased and the ROS level of bone marrow cells increased significantly in the irradiated group than those of control group (P＜0.05). Compared to 2 Gy irradiation group, the numbers of WBC, PLT, BMNCs and CFU-GM were significantly increased in 2 Gy irradiation +DIM group, and the level of ROS was decreased significantly (P＜0.05). Conclusion DIM has a protective effect on hematopoietic cells following radiation-induced injury, which may be related with the decreased ROS level. Related Articles | Metrics

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The preparation and feasibility study of composite urethral stent-rabbit oral mucosal epithelial cells and PCL electrospun fiber GUAN Yong, MENG Qing-ya, XU Guo-dong, ZHANG Fu-yi, SHU Jian-bo 2017, 45 (6):  584-587.  doi: 10.11958/20161525 Abstract ( 833 )   PDF (665KB) ( 3408 )   Objective To explore the feasibility of using composite scaffolds of rabbit oral epithelial cells and polycaprolactone (PCL) electrospun fibers for urethral repair. Methods The 25% PCL was prepared using a 5∶1 by volume mixture of trichloromethane and anhydrous methanol, and PCL fiber tubular scaffolds were obtained by electrospinning. Rabbit oral mucosa epithelial cells (1.5×105) were implanted on the PCL scaffold. Subsequently, they were embedded in nude mice subcutaneous, explanted in 2 weeks. PCL fiber tubular scaffolds without rabbit oral mucosa epithelial cells were used as control. The complex urethral scaffolds were evaluated by immunofluorescence staining with cytokeratin antibody and HE staining. Results Compared with blank PCL group, the rabbit oral mucosa epithelial cell group showed a good cellularization. Rabbit oral mucosa epithelial cells formed a dense cell layer on the surface of PCL lumen, which suggested that rabbit oral mucosa epithelial cells can proliferate on the surface of PCL lumen. Conclusion Rabbit oral epithelial cells can be used as one of the seed cells for tissue engineered urethral scaffolds, and it is possible to construct tissue engineering substitute materials for urethral repair by rabbit oral epithelial cells combined with PCL. Related Articles | Metrics

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Effects of edaravone on JAK2/STAT3 signaling pathway in transplanted donor liver of rats with different cold ischemia times XUE Pei-tong1, ZHANG Quan-sheng2, LI Xin3, LI Hong3△ 2017, 45 (6):  588-592.  doi: 10.11958/20170174 Abstract ( 802 )   PDF (671KB) ( 3593 )   Objective To investigate the effect of edaravone on the JAK2/STAT3 signaling pathway after ischemiareperfusion injury in donor rat liver under different cold ischemia times. Methods A total of 102 SD rats were randomly divided into sham operation group，control group and experimental group. Six rats were in sham operation group with free liver operation and no transplantation. Forty- eight rats were in control group and experimental group respectively, and divided into subgroups according to the different cold ischemia times (30 min, 6 h, 12 h and 18 h). There were 6 donors and 6 recipients in each group. The rat model of orthotopic liver transplantation was established by modified "two cuff method". All the donors were perfused by abdominal aorta and the warm ischemia time was 3-5 min. After different cold ischemia times, the experimental group was treated with edaravone (3 mg/kg) at 5 min before the opening of the new hepatic artery, and control group was injected with 3 mg/kg saline. Recipients of each group were sacrificed after 6 h. Finally, real-time fluorescence quantitative PCR was used to analyze the relative expression of JAK2/STAT3 mRNA of donor liver. Results The GAPDH gene and JAK2/STAT3 were well amplified. Under the same cold ischemia time, compared with the control group, the relative expression of JAK2/STAT3 was significantly decreased in the experimental group (P＜0.05). With the prolongation of cold ischemia time, the relative expressions of JAK2 and STAT3 mRNA showed a decreasing trend in control group and experimental group, while the relative expression of JAK2 mRNA increased first and then decreased in the experimental group (P＜0.05). Conclusion Edaravone has a protective effect on transplanted donor liver during different cold ischemia times, and extends the cold ischemia time for 18 h, which may be related to the inhibition of JAK2/STAT3 signal transduction pathway. Related Articles | Metrics

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Effects of chrysophanol on NO of brain tissue and anti-anoxia in mice with cerebral ischemia-reperfusion injury WANG Si-hai1, LIANG Chun-li1, ZHANG Hai-hong2, WANG Shu2△ 2017, 45 (6):  593-595.  doi: 10.11958/20170078 Abstract ( 823 )   PDF (324KB) ( 3719 )   Objective To study the effects of chrysophanol（Chry）on NO of brain tissue and anti-anoxia in mice with cerebral ischemia-reperfusion injury. Methods A total of 75 SPF Kunming mice were randomly allocated into five groups: sham operation group, ischemia-reperfusion group, high-dose group (Chry 10.0 mg·kg- 1), medium-dose group (Chry 1.0 mg · kg- 1) and low-dose group (Chry 0.1 mg·kg- 1). Using improved Himori method, cerebral ischemia reperfusion-injury model was produced in conscious mice by temporarily obstructing bilateral common carotid arteries. The neurological function was measured according to the Bederson scoring standard. The mice were subjected to decapitation for hypoxia tolerance test. The gasping time was measured by anoxia tolerance test in beheaded mice. The level of NO in cerebrum was detected. Results Chrysophanol can decrease the level of NO in cerebrum of mice with cerebral ischemia- reperfusion injury and prolong the gasping time in beheaded mice with cerebral ischemia-reperfusion injury [low-dose group, (14.6±1.2) s; medium- dose group, (16.4 ± 1.2)s; high- dose group, (17.4 ± 1.1)s; ischemia-reperfusion group, (13.2 ± 1.0)s, P＜0.05]. Conclusion The protective effects of chrysophanol on cerebral ischemia-reperfusion injury are involved in decreasing the content of NO in brain tissue and anti-anoxia in mice. Related Articles | Metrics

Clinical Study

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The relationship of serum levels of FSH, LH and PRL and clinicopathological features and prognosis in patients with serous ovarian cancer CUI Lei, GUO Fei, YAN Ye, PAN Ming-xia, DONG Yang-yang, XUE Feng-xia△ 2017, 45 (6):  596-600.  doi: 10.11958/20170184 Abstract ( 1389 )   PDF (334KB) ( 3590 )   Objective To investigate the relationship between serum follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL) and clinicopathological features and prognosis of serous ovarian cancer retrospectively. Methods A total of 73 patients with serous ovarian cancer treated in the Department of Obstetrics and Gynecology of Tianjin Medical University General Hospital from January 2000 to December 2015 were included in this study. The relationship between serum FSH, LH, PRL and clinicopathological features was analyzed by Mann- Whitney U method. Kaplan-Meier (K-M) method was used to analyze survival rates of patients with different clinical features. Multivariate Cox proportional hazards regression analysis was used to analyze prognostic factors of serous ovarian cancer patients. Results The mean concentrations of serum FSH and LH were significantly higher in the >50 year-old group than those in the <50 year-old group (P＜0.05). The mean concentrations of FSH and LH were significantly higher in menopause group than those in non - menopause group (P＜0.05). There were no significant differences in serum levels of FSH and LH in patients with other different clinicopathological features (P＞0.05). There was no significant correlation between serum PRL concentration and clinicopathological features (P＞0.05). Analysis results showed that poor prognosis of patients was related with high serum levels of FSH (>40.13 IU/L), PRL (>14.96 μg/L) and FIGO stage ( Ⅲ + Ⅳ) (P＜0.05). There was no significant correlation between serum LH concentration and prognosis (P＞0.05). COX regression analysis showed that the serum PRL> 14.96 μg/L was risk factor for prognosis of serous ovarian cancer [HR（95% CI）: 3.530（1.180- 10.557)，P=0.024]. Conclusion The serum levels of FSH and LH are significantly increased in postmenopausal women than those in menopause women. The serum level of PRL is correlated with the prognosis of serous ovarian cancer. Related Articles | Metrics

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The relationship between vascular lesion and serological changes in patients with coronary heart disease and obstructive sleep apnea hypopnea syndrome XIE Cun, WANG Ming-hui, HU Yue-cheng, CONG Hong-liang△ 2017, 45 (6):  601-604.  doi: 10.11958/20170343 Abstract ( 802 )   PDF (326KB) ( 3751 )   Objective To investigate the relationship between vascular lesion and serological changes in patients with coronary heart disease (CHD) complicated with obstructive sleep apnea hypopnea syndrome (OSAHS). Methods According to the standard, a total of 168 patients of OSAHS complicated with CHD were selected in this study. Those patients were divided into 3 groups according to the apnea hypopnea index (AHI) level: light group (AHI, 5-14/h), moderate group (AHI, 15-30/h) and severe group (AHI, ＞30/h). Syntax scores were performed on three groups according to coronary angiography results. The data of hemoglobin (Hb), platelet count (PLT), fibrinogen (FIB), D- Dimer (DD), total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL), triacylglycerol (TG), alanine aminotransferase (ALT), aspartate transaminase (AST), uric acid (UA), creatinine (Cr) and echocardiographic examination index were collected and analyzed in three groups. Results The Syntax score was significantly higher in severe group than that in mild and moderate groups (P < 0.05). There was no significant difference in the Syntax score between mild and moderate groups. The levels of Hb, FIB, LDL, pulmonary arterial pressure (PAP), and right ventricle transverse diameter (RVD) were significantly lower in the mild and moderate groups than those in the severe group (P < 0.01). The left ventricular ejection fraction (LVEF) was significantly lower in the severe group than that in the mild and moderate groups (P < 0.01). There were no significant differences in levels of Hb, FIB, LDL, PAP, RVD and LVEF between mild group and moderate group (P > 0.05). Conclusion The serology and cardiac structure can change gradually in severe OSAHS patients, and the coronary artery lesion will be more complex. Therefore, the clinical treatment should pay attention to screening for OSAHS in patients with coronary heart disease. Related Articles | Metrics

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The study of the theta oscillation characteristic in working memory in patients with mild cognitive impairment LI Hua-juan1, ZHU Jing1, ZHANG Ting2, SONG Yi-jun1△ 2017, 45 (6):  605-609.  doi: 10.11958/20170091 Abstract ( 990 )   PDF (471KB) ( 3740 )   Objective To study the feature of theta oscillation during working memory (WM) dysfunction in patients with mild cognitive impairment (MCI). Methods Fifteen MCI patients (MCI group) and 15 healthy subjects for checkup (control group) in Tianjin Medical University General Hospital from January to November 2016 were recruited. The differences of behavioral results in visual WM tasks were compared between the two groups. Thirty four- channel electroencephalogram (EEG) signals were recorded simultaneously. Short- Time Fourier transform was used to calculate spatial and time-frequency distribution of power and spectral coherence of multi-channel EEGs. Results Compared with control group, MCI group showed significantly longer reaction time and lower accuracy in WM tasks (P＜0.01). The analysis of EEGs revealed that the power of Fz channel was the biggest at frontal midline in both groups. And the power density of theta oscillation was lower at frontal electrode sites in MCI group than that of control group ( P＜0.01), and theta coherence was significantly decreased in frontal region and between frontal-posterior regions ( P＜0.05). Conclusion The absence of theta oscillaton in frontal region may be one of the important reasons of working memory dysfunction for MCI. Related Articles | Metrics

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Effects of butylphthalide on serum inflammatory cytokines and oxidative stress products in patients with acute cerebral infarction AN Wen-feng, ZHAI Lu-hui△, MA Yu-chao, GAO Peng-fei 2017, 45 (6):  610-613.  doi: 10.11958/20170308 Abstract ( 918 )   PDF (326KB) ( 4372 )   Objective To investigate the effects of butylphthalide injection on serum levels of inflammatory factors and oxidative stress products in patients with acute cerebral infarction. Methods A total of 120 patients with acute cerebral infarction were selected in our hospital from March 2014 to September 2016. Patients were divided into conventional treatment group (n=56) and butylphthalide group (n=64) according the therapeutic methods. The conventional treatment group was treated with improving microcirculation and resisting blood platelet aggregation, while the butylphthalide group was injected with butylphthalide (100 mL intravenous drip, twice a day) on the basis of conventional treatment. The levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-17, IL-23 and methane dicarboxylic aldehyde (MDA) were detected before the treatment and 7 days,14 days after the treatment. The clinical efficacy was also accessed. Results The TNF-α, IL-6, IL-17, IL-23 and MDA levels were significantly decreased 7 days and 14 days after the treatment than those before the treatment in two groups of patients (P < 0.05). And the above indexes were also decreased in butylphthalide group than those of conventional treatment group (P < 0.05). No obvious adverse reactions were observed in two groups. Conclusion Butylphthalide can improve the clinical symptoms through the inhibition of inflammation and oxidative stress in patients with acute cerebral infarction. Related Articles | Metrics

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The middle and long term curative effects of percutaneous minimally invasive surgical treatment on lumbar spinal tuberculosis ZHU He-tao1,2, ZHU Bo1, GAO Tian-yang1, ZHANG Lin3, DENG Xiao-lei4, ZHANG Xi-feng2△ 2017, 45 (6):  614-619.  doi: 10.11958/20170219 Abstract ( 1090 )   PDF (1080KB) ( 3669 )   Objective To explore the application value of minimally invasive surgery in the treatment of lumbar spinal tuberculosis, and to provide reference for clinical treatment of spinal tuberculosis. Methods Data of 252 cases of patients with lumbar spinal tuberculosis treated by conservative treatment in our hospital from January 2005 to December 2014 were retrospectively analyzed. Patients were divided into four groups on the basis of systemic application of antituberculosis chemotherapy. A total of 154 patients were given simple local chemotherapy of percutaneous placement of focus catheter (group A), 48 patients were received percutaneous perfusion drainage and local chemotherapy (group B), 32 patients underwent percutaneous puncture catheter debridement combined with local chemotherapy (group C), and 18 patients were given percutaneous debridement and internal fixation combined with local chemotherapy catheter (group D). Data of erythrocyte sedimentation rate (ESR), visual analogue scale (VAS), Oswestry disability index (ODI) score and the modified MacNab criteria were recorded before operation and at the end of the follow-up in four groups of patients. Results Of the 252 patients, 228 were followed up and 214 patients achieved clinical cure. The lost access were15 cases in group A, 5 cases in group B, 2 cases in group C and 2 cases in group D. The total rate of lost visit was 9.52%. The follow up duration ranged from 25-126 months. The mean duration of follow-up was 68(60, 76) months. A total 214 cases reached the standard of clinical cure. No complications (retrograde infection and cross infection) were found in all patients during treatment. ESR was statistically decreased to (7.26±3.43) mm/1 h at the last follow-up (t= 35.06, P = 0.023) compared with that (44.96± 12.42) mm/1 h before operation. The VAS and ODI were 1.5(1, 3) and 30(25, 35)% at the last follow- up, which were significantly improved than those [7.5(7.0, 8.0) and 60(55, 65)% ] before operation (Z=13.641 and 6.806, P＜0.05). According to the improved MacNab criteria, the overall excellent and good rates for patients were 86.4% (197/228) at the last follow- up. Conclusion According to the stepped care and personalized treatment, patients of lumbar tuberculosis are preoperative comprehensive evaluated, and most patients can achieve long-term stability and a better clinical efficacy after interventional and minimally invasive treatment. Related Articles | Metrics

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The application of endoscopic sphincterotomy combined with endoscopic papillary large balloon dilation in the treatment of choledocholithiasis MEN Chang-jun，ZHANG Guo-liang△ 2017, 45 (6):  620-623.  doi: 10.11958/20170499 Abstract ( 1154 )   PDF (315KB) ( 3745 )   Objective To investigate the effect of endoscopic sphincterotomy（EST) combined with endoscopic papillary large balloon dilation（EPLBD) in the treatment of common bile duct stones. Methods A total of 128 patients treated with EST combined with EPLBD for common bile duct stones were divided into 0 min group, 1 min group, 3 min group and 5 min group, according to the different balloon expansion times. The balloon was extended to the required diameter and was maintained to the corresponding time, and then decreased the pressure to remove the balloon. Patients were given routine monitoring and treatment after operation. All patients were observed more than 48 h to determine whether there were early complications such as pancreatitis and bleeding. The effectiveness of EPLBD was evaluated by the stone diameter, expansion balloon diameter, ERCP operation time and use of stone breaker. The safety was evaluated by the occurrence of complications including postoperative pancreatitis, bleeding and perforation. Results All patients were successfully removed stones. There were no significant differences in the maximum diameter of stone, the diameter of bile duct dilatation and the diameter of balloon dilatation between groups. No bleeding, perforation, biliary tract infection and acute pancreatitis were found in patients. There were no significant differences in the utilization rates of stone breaker between groups. ERCP operation times showed a trend of decreased first and then increased in 0, 1 and 3 min groups (P＜ 0.05). There was no significant difference in ERCP operation time between 3 min group and 0 min group. There were no significant differences in incidence rates of high amylase between groups. Conclusion EST combined with EPLBD is a safe and effective treatment for early stage of larger common bile duct stones, of which 1 minute group shows better effect. Related Articles | Metrics

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Clinical study on blood glucose level and blood pressure variability in hypertensive patients GU Li, YU Yi-jun△, XU Yan-ling, WU Hui-jun, ZHENG Wei, ZHOU Zhi-yun 2017, 45 (6):  624-627.  doi: 10.11958/20170392 Abstract ( 826 )   PDF (333KB) ( 4334 )   Objective To explore the relationship between blood glucose level and blood pressure variability in hypertensive patients. Methods A total of 125 hypertensive patients hospitalized in the department of cardiology in our hospital during May 2015 to October 2016 were retrospectively analyzed. Patients were divided into hypertension with diabetes mellitus group (n=59) and hypertension without diabetes mellitus groups (n=66). Data of blood pressure, blood pressure variability and blood glucose levels were analyzed in the two groups. Results (1) Hyperlipidemia, low density lipoprotein and cholesterol levels were significantly lower in the hypertension with diabetes mellitus group than those of hypertension without diabetes mellitus group. Level of glycosylated hemoglobin (HbA1c), 24 h diastolic pressure variability coefficient (24 hDBPCV), daytime systolic blood pressure variability coefficient (dSBPCV), and daytime diastolic pressure variability coefficient (dDBPCV) were significantly higher in the hypertension with diabetes mellitus group than those of hypertension without diabetes mellitus group (P<0.05). (2) Spearman correlation analysis showed that hypertension with diabetes mellitus was positively correlated with 24 hDBPCV, dSBPCV and dDBPCV. (3) ROC curves of HbA1c for diagnosis of non-dipper blood pressure in hypertensive patients showed that the cut-off value of HbA1c was 5.85% and the area under curve was 0.692 (P<0.05). The sensitivity was 71.1% and the specificity was 63.7% . Conclusion The present results demonstrate that blood pressure variability is increased in hypertension patients with higher blood glucose. HbA1c level has potential clinical value for diagnosis of non-dipper blood pressure in hypertensive patients. Related Articles | Metrics

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Comparative analysis of CT images of non-tuberculous mycobacterium tuberculosis and multidrug-resistant pulmonary tuberculosis YU Ting-shan, SHEN Xiao-lan, LONG Xian-rong, MENG Jia-xiao, CHEN Xun 2017, 45 (6):  628-631.  doi: 10.11958/20170018 Abstract ( 1533 )   PDF (678KB) ( 4109 )   Objective To study the computed tomograhpy (CT) features of non-tuberculous mycobacteria (NTM) and multidrug-resistant pulmonary tuberculosis (MDR-TB), and to improve the differential diagnosis of the disease. Methods The CT imaging data of 67 patients diagnosed with NTM pulmonary disease (NTM lung disease group) and 103 patients with MDR-TB (MDR-TB group) were selected from January 2010 to December 2015 in our hospital. The imaging findings and differences in lesion location were compared between two groups. Results Lesions of NTM lung disease occurred in the posterior segment of the posterior and posterior lumbar dorsal (Ⅰ area), clustered lobular central nodules, accompanied by bronchiectasis and subpleural thin wall empty, rare bronchial foci. MDR-TB lesions occurred in the middle lobe of the right lung and the upper lobe of the left lung (Ⅱ area). The upper lung of the lungs were patch, nodules and caseous lesions, with thick wall and chronic lung inflammation, showing thick wall empty, pulmonary consolidation, atelectasis, calcification (lung), hilar mediastinal lymph node calcification, lung volume reduction, pleural thickening and pleural effusion. Conclusion Chest CT images are similar in NTM lung disease and MDR- TB, but there are differences. The detailed analysis of image features can provide a basis for clinical differential diagnosis. Related Articles | Metrics

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The anesthesia of stent implantation for abdominal aortic aneurysm after long term heart transplantation ZHANG Zhi-qiang，ZHANG Shan△，MENG Li-jiang 2017, 45 (6):  632-633.  doi: 10.11958/20170233 Abstract ( 1094 )   PDF (672KB) ( 3814 )   There are different degrees of cardiac allograft vasculopathy in patients with long term cardiac transplantation. Coronary angiography shows partial or diffuse coronary artery stenosis, which is one of the main causes of death. There are few typical symptoms of angina pectoris because of no autonomic innervation in transplanted heart. The first symptom to the end of the disease is severe congestive heart failure or arrhythmia. So the principles of diagnosis and treatment in long- term survivors should be in accordance with the coronary heart disease treatment. In this paper, we reported a case of anesthesia of a patient underwent stent implantation for abdominal aortic aneurysm after 8 years of cardiac transplantation. The preoperative preparation and perioperative management were performed according to the principle of coronary heart disease. The patient passed through the perioperative period safely. Related Articles | Metrics

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A case of lung enteric adenocarcinoma along with infiltrating lung adenocarcinoma WANG Xing1, WANG Jing2 2017, 45 (6):  634-636.  doi: 10.11958/20170256 Abstract ( 2608 )   PDF (597KB) ( 4276 )   The intestinal type of lung adenocarcinoma is a very rare subtype of lung adenocarcinoma, presenting with pulmonary symptoms, but without any gastrointestinal symptoms and pathological changes. The author found 1 case of double primary lung adenocarcinoma of the left lung, which was confirmed by surgery pathology, one for lung adenocarcinoma, one for a rare type of intestinal adenocarcinoma. This paper reviews the relevant literatures of the diagnosis and treatment of this case. Related Articles | Metrics

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A case report of right coronary artery bypass grafting with completely reversed internal CHAI Jun-wu1, WANG Kai1, ZHOU Wei1, CHEN Hong-lei1, XUE Fen-long1, WANG Wei-tie1, MI Rui2, KONG Xiang-rong1△ 2017, 45 (6):  636-637.  doi: 10.11958/20170310 Abstract ( 1150 )   PDF (390KB) ( 3647 )   The incidence of dextrocardia is lower. The dextrocardia is often associated with congenital anatomical abnormalities. The patient with normal cardiac structure of dextrocardia and severe coronary heart disease that needs coronary artery bypasss is rarer. This article summarizes the perioperative management and surgical experience of dextrocardia with bypass surgery via a clinical case. Related Articles | Metrics

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The standard operating techniques for diagnostic interventional pulmonology based on rapid on-site evaluation FENG Jing1, ZHOU Guo-wu2, LI Wen3△, MENG Chen4△, ZHOU Hong-mei5△, LI Cai-li1, CAO Jie1 2017, 45 (6):  638-642.  doi: 10.11958/20170514 Abstract ( 1034 )   PDF (415KB) ( 3579 )   With the organic combination of rapid on-site evaluation (ROSE) and interventional pulmonary diagnostic technology, we can build a complete "The System of Diagnostic Interventional Pulmonology Based on Rapid on- site Evaluation". With the help of ROSE, changing the ways, methods and modalities of interventional pulmonary diagnostic technology to obtain the target lesions is the core of this system. In this statement, the most commonly used standard operating techniques in "The System of Diagnostic Interventional Pulmonology Based on Rapid on- site Evaluation" are described in detail, including double-hinge curette operating technique, transbronchial lung biopsy (TBLB) technique, and transbronchial brushing technique. Related Articles | Metrics

Diagnostic Techniques

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Comparison of contrast-enhanced ultrasound and contrast-enhanced helical computed tomography in diagnosis of hepatocellular carcinoma SHI Jing-xiang, WANG Yi-jun, JING Xiang, WANG Feng-mei, DING Jian-min, ZHANG Xiang, ZHANG Qin 2017, 45 (6):  643-647.  doi: 10.11958/20170410 Abstract ( 1155 )   PDF (659KB) ( 4002 )   Objective To compare the diagnostic value of contrast- enhanced ultrasound (CEUS) and contrastenhanced helical computed tomography (CECT) for hepatocellular carcinoma (HCC) with liver cirrhosis. Methods Two hundreds and forty-one focal liver lesions in 207 patients with Hepatitis B virus (HBV) cirrhosis were detected with CEUS and CECT, respectively. Pathological results were used as "gold standard" to compare the two methods. Diagnostic results of the two methods were compared with pathological results. Differences were assessed using the McNemar test, and the Kappa test was used for consistency evaluation. Results (1) For 113 liver lesions that were ≤ 2 cm, the number of HCC lesions was 63, and the number of benign lesions was 50. There were no significant differences in results of CEUS and CECT compared with that of the "gold standard" of McNemar test results (P = 0.824, P = 0.082). Consistency of the Kappa test results of CEUS and CECT in comparison with the "gold standard" was general (Kappa = 0.643, Kappa = 0.421). The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of HCC diagnosed by CEUS were higher than those of CECT. The rate of arterial enhancement was better for CEUS [87.30% (55/63)] than that for CECT [69.84% (44/63), χ2 = 5.704, P = 0.017]. (2) For 128 liver lesions that were > 2 cm, the number of HCC lesions was 77, and the number of benign lesions was 51. There were no significant differences in the diagnostic results between McNemar test and CEUS and CECT tests (P = 0.481, P = 0.167). Consistency of the Kappa test results of CEUS and CECT and "gold standard" was general (Kappa = 0.710, Kappa = 0.697). The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of HCC were not different between two diagnostic methods. The rate of arterial enhancement was 89.61% (69/77) for CEUS and 85.71% (66/77) for CECT, and there was no significant difference between the two groups (χ2 = 0.540, P = 0.462). Conclusion For HCC ≤ 2 cm, the diagnostic performance of CEUS is better than that of CECT. For HCC > 2 cm, the diagnostic performance is similar for the two diagnostic methods. Related Articles | Metrics

Epidemiological Investigation

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Pathogenic analysis of rat infected hantavirus in Henan province, 2014-2016 DU Yan-hua, LI Yi, MA Hong, WANG Hai-feng, XU Bian-li, HUANG Xue-yong△ 2017, 45 (6):  648-651.  doi: 10.11958/20170418 Abstract ( 982 )   PDF (395KB) ( 3742 )   Objective To analyze the genotyping of hantavirus and investigate the pathogenic features of local rats in Henan province. Methods A total of 600 rats captured in Queshan county, Zhumadian city from 2014-2016 were chosen to find out the major species and density. Rat lung specimens were detected by RT-PCR using partial M and S segment primers, then sequencing and phylogenetic analysis based on M segment (2 003-2 302 nt) were performed to analyze gene subtype and evolution. Results In the field of Queshan county, major species were sewer rats and apodemus agrarius, and the average density of rats was 1.33%-1.83%. Sewer rats, mus musculus and apodemus agrarius were major species in the residential area, and the average density of rats was 1.36%- 1.97% . Hantaviruses were detected by RT- PCR in three captured rats in 2014, and the species were mus musculus, cricetulus triton and sewer rats. Nucleotide homology similarity based M and S segment of three positive products was 100%. Phylogenetic analysis indicated the virus was belonged to S4 subgenotype of Seoul virus, which was similar with the strains in Korea and Hubei province, China. Conclusion The virus from rats in Queshan county, Henan province is seoul virus, S4 subgenotype. It is necessary to take the relevant prevention and control measures to prevent hemorrhagic fever of renal syndrome because of wide host range. Related Articles | Metrics

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Analysis on epidemiological characteristics of bacterial dysentery and Shigella detection results in Tianjin city during 2008-2014 GAO Lu, ZHANG Wei 2017, 45 (6):  651-656.  doi: 10.11958/20170143 Abstract ( 929 )   PDF (507KB) ( 3541 )   Objective To understand the epidemiological characteristics of bacillary dysentery and etiological characteristics of Shigella in Tianjin, and to provide evidence for the prevention and control measures. Methods The data of disease surveillance information reporting system from 2008 to 2014 were analyzed retrospectively. A total of 3 955 stool samples came from the clinically diagnosed cases of bacillary dysentery were collected, according to the extraction method of stratified sampling in Tianjin city, suburban area, Binhai New Area and suburban area sentinel hospitals, and according to the sampling interval for the May to October from 2008 to 2014. Samples were detected and cultured for Shigella flora. Results (1) Epidemiological characteristics: from 2008 to 2014, a total of 65 179 cases of bacillary dysentery were reported in Tianjin city, with an average annual incidence of 72.00/100 thousand. The annual incidence rate showed a downward trend year by year. The incidence was a downward trend, including urban (120.28/100 thousand), suburban areas (70.36/100 thousand), Binhai New Area (64.22/100 thousand) and the outer suburbs (19.39/100 thousand). The onset time was mainly concentrated in June-September, and the peak incidence occurred in August. The incidence rate was higher in people below 5 years old and above the age of 85. The annual incidence rate was higher in male patients of 0 to 24 years old and above 75 years old than that of female. The annual incidence rate was higher in female patients of 50 to 74 years old age group than that of male (P< 0.05). The distribution of occupation was scattered among children and retirees. (2) Distribution of bacteria: the 229 Shigella strains were positive (5.79% ), including 136 strains of Shigella sonnei (59.39% ), 93 strains of Shigella flexneri (40.61%). The 89 strains of Shigella flexneri (4 strains were not detected) were detected in 8 subtypes, F2a (49.44%), F2b (22.47%) and FX (13.48%). The subtypes of Shigella flexneri were detected decreased year by year from 2008 to 2014, showing a single subtype. The highest detection rate of Shigella was found in 10-19 years old (9.55%). The S.sonnei positive rate was the highest in urban (5.09%), and the Shigella flexneri positive rate was the highest in suburban areas (3.09%). Conclusion The key population for the prevention and control of bacillary dysentery in Tianjin is scattered children and old people. It is necessary to strengthen the propaganda and health education to improve the health consciousness of the key population and their daily nursing staff. With the continuous changes of Shigella flora, it is necessary to carry out long-term monitoring to find out the regularity of shigella. Related Articles | Metrics

Review

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Recent advances in histological changes of high myopia LI Wen-bo, HU Bo-jie, LI Xiao-rong△ 2017, 45 (6):  657-659.  doi: 10.11958/20170441 Abstract ( 1241 )   PDF (291KB) ( 3675 )   The incidence of high myopia has increased yearly, which has been an area of focus for investigation. The complications of high myopia are contributing factors to blindness，such as posterior staphyloma, chorio- retinal atrophy, choroidal neovascularization, optic nerve head and macular defects. The study of histological changes helps clarify the mechanism. With the development of modern technology such as spectral- domain optical coherence tomography and enhanced depth imaging-technique, histological changes in high myopia can be measured accurately. The purpose of this review is to provide an update on various pathologic changes, including in sclera, Choroid/Bruch’s membrane, optic nerve, vitreous and retina. Related Articles | Metrics

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Whole exome sequencing in the application of hereditary breast cancer susceptibility gene discovery ZHOU Nan1, YANG Kang2△ 2017, 45 (6):  660-662.  doi: 10.11958/20161587 Abstract ( 1198 )   PDF (303KB) ( 3826 )   Genetic susceptibility factor is one of the important reasons to induce breast cancer. Breast cancer risk variants are divided into three categories including high, moderate and low penetrances. Traditional BC susceptibility gene discovery approaches limit the search for breast cancer susceptibility genes with high and moderate risk variants. Whole exome sequencing technology provides a quick and efficient method to discover breast cancer susceptibility genes. At present, a number of breast cancer susceptibility genes have been identified by whole exome sequencing method, which provides useful guidance for the risk assessment and pathogenesis of hereditary breast cancer. In this paper, we reviewed the whole exome sequencing technology and discussed the experimental design, data filtering strategy, statistical significance and correlation analysis. Related Articles | Metrics

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Research progress of platelet function tests in antiplatelet effect on monitoring P2Y12 receptor antagonist KONG Fan-qiang, REN Jing, MEN Jian-long△ 2017, 45 (6):  663-667.  doi: 10.11958/20170185 Abstract ( 1526 )   PDF (346KB) ( 3822 )   The P2Y12 receptor antagonist is used widely in prevention and treatment of cardiovascular and cerebrovascular disease. Monitoring changes of platelet function after treatment can improve the prognosis of patients. The platelet function test is the important way to evaluate high residual platelet reactivity after antiplatelet treatment, including light transmission aggregometry (LTA), whole blood impedance aggregometry assay (WBIA), vasodilator- stimulated phosphoprotein (VASP), thrombelastogram (TEG), platelet function analyzer- 100 (PFA- 100) and VerifyNow system (VerifyNow). It is very different for the reflecting ability with residual reactivity of platelets among these tests after antiplatelet therapy, and also significant difference for assessment effect. Among them, LTA is a classic method for the curative effect evaluation of anti- platelet agents, which is convenient and cheap, but it is susceptible to the operating and environment interference. The clinical application of WBIA is less, and which lacks threshold value for assessment. VASP is sensitive for the changes of platelet function, but the test is complex and expensive. TEG can monitor the inhibition ratio of drugs on anti- platelets, but it needs to verify the safety of treatment. It is not clear for sensitivity and specificity with monitoring anti-platelet agent by PFA-100. VerifyNow is effective and reliable, but the cost is high. The evidence of clinical study shows that LTA, VASP and VerifyNow can reflect the effect of platelet inhibition of P2Y12 receptor antagonists sensitively, and is associated with the risk of major adverse cardiac events (MACE) in patients with cadiovascular diseases. Related Articles | Metrics

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The new progress of intestinal microbiome and lung diseases YU Hong-zhi, WU Qi 2017, 45 (6):  668-672.  doi: 10.11958/20170373 Abstract ( 2187 )   PDF (413KB) ( 4455 )   Intestinal microflora is a general term for microbial communities that reside in the human intestine. In recent years, the research on diseases related to intestinal microbiota has developed rapidly. It is found that intestinal microbiota is closely related to digestive tract diseases, metabolic diseases, immune diseases, mental diseases, and lung diseases. Intestinal dysbacteriosis, mucosal barrier damaging and local immune dysfunction may be involved in a variety of disease processes. In addition, respiratory diseases such as asthma and pneumonia seriously affect human health. The intestinal microbiota is crucial in regulating immune response of lung to pathological mechanism of diseases including asthma, pneumonia and pulmonary tuberculosis. Therefore, it may be a new direction for the treatment of lung diseases by understanding intestinal microbiota in lung diseases, and identifying, describing and manipulating immunity network of pulmonary disease. Related Articles | Metrics