Table of Content

15 July 2022, Volume 50 Issue 7 Previous Issue    Next Issue

---

For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

Select

Ligand binding characteristics of signal molecule 3-O-C12-HSL and PPAR γ-LBD in pseudomonas aeruginosa ZHANG Yunyan, MA Liuheyi, CHEN Minyi, LI Youqiang, Liu Runmei, LUO Dongyuan 2022, 50 (7):  673-677.  doi: 10.11958/20212782 Abstract ( 478 )   PDF (1205KB) ( 2250 )   Objective　To prepare purified human peroxisome proliferator-activated receptor-ligand binding domain (PPARγ-LBD) peptide segment and study the binding strength of N-3-oxododecanoyl-l-homoserine lactone (3-O-C12-HSL) to PPARγ-LBD. Methods　The binding site of 3-O-C12-HSL to PPARγ was predicted by molecular docking. PPARγ-LBD gene was synthesized and inserted into pET28b plasmid. The recombinant peptide fragment was induced by IPTG after identification and analyzed by SDS-PAGE and Western blot assay. The binding kinetic parameters of 3-O-C12-HSL and PPARγ-LBD were measured by surface plasmon resonance technique. Results　Results of molecular docking showed that 3-O-C12-HSL formed hydrogen bond with Tyr473 and Tyr327 of PPARγ-LBD, and the terminal flexible carbon chain of 3-O-C12-HSL formed hydrophobic interaction with the residues of Met329 and Leu330 of PPARγ-LBD. The human PPARγ-LBD peptide fragment was prepared, and the molecular weight of PPARγ-LBD was 25~35 ku consistent with that of Western blot assay and SDS-PAGE. The binding affinity constant of 3-O-C12-HSL with PPARγ was 1.65×10-4 mol/L, the binding rate constant was 1.06×102/ms, and the dissociation rate constant was 1.75×10-2/s. Conclusion　The binding ability of 3-O-C12-HSL to PPARγ-LBD functional region is stronger than that of PPARγ-specific antagonist GW9662, which provides experimental basis for preparing specific targeted drugs against 3-O-C12-HSL. Related Articles | Metrics

Select

Effects of Mtb-infected type Ⅱ alveolar epithelial cells-derived exosome on the polarization of macrophages through miR-145 LI Jianjun, WU Sufang, BAI Fengxi 2022, 50 (7):  678-685.  doi: 10.11958/20220154 Abstract ( 592 )   PDF (1104KB) ( 2127 )   Objective　To investigate the effect of Mycobacterium tuberculosis (Mtb)-infected type Ⅱ alveolar epithelial cells (AECⅡ)-derived exosomes (Exos) on the polarization of macrophages. Methods　Experiment 1: AECⅡ and Mtb strain H37Rv were cultured in vitro. AECⅡ was infected with Mtb and Exos, and was isolated. Exos were divided into the AECⅡ group and the Mtb-AECⅡ group. Transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA) were used to observe the morphology of Exos and to measure the number and size of Exos. Flow cytometry was used to identify the expressions of Exos surface characteristic markers CD63, CD81 and HSP70. BCA method was used to measure the protein content of Exos. miRNA microarray was used to detect and verify the differential miRNA expression profile between the two groups. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6) and IL-10 in the cell culture supernatant. Experiment 2: AECⅡ was separated into the control group, the mimic-NC group, the miR-145 mimic group, the inhibitor-NC group and the miR-145 inhibitor group. After AECⅡ was transfected with lentivirus that overexpressed or silenced miR-145, AECⅡ was infected by Mtb, and then Exos were isolated. Real-time fluorescence quantitative PCR (qPCR) was used to measure the level of miR-145 in Mtb-AECⅡ Exos after transfection. Flow cytometry was used to detect the expression levels of M1 type marker (CD86) and M2 type marker (CD163) of macrophages, and qPCR was used to detect the levels of miR-145, TNF-α, inducible nitric oxide synthase (iNOS), arginase 1 (Arg-1) and IL-10 mRNAs in macrophages. Results　Experiment 1: Mtb-infected AEC Ⅱ secreted Exos as spherical vesicles with a diameter of about 100 nm. CD63, CD81 and HSP70 were all positive in Exos. Compared with the AECⅡ group, the quantity and protein content of Exos, levels of miR-145 and IL-10 were increased in the Mtb-AECⅡ group (P＜0.05), and levels of TNF-α and IL-6 were decreased (P＜0.05). Experiment 2: compared with the control group, the level of miR-145 in Mtb-AECⅡ Exos, the percentage of CD163-positive macrophages, the levels of miR-145, Arg-1 mRNA and IL-10 mRNA in macrophages were increased in the miR-145 mimic group (P＜0.05), and the percentage of CD86-positive macrophages, the levels of TNF-α mRNA and iNOS mRNA in macrophages were reduced (P＜0.05). Changes of the above indicators in the miR-145 inhibitor group were opposite to those in the miR-145 mimic group. Conclusion Mtb infection with AECⅡ-derived Exos may stimulate M2 type polarization of macrophages and inhibit M1 polarization through miR-145, thus resisting inflammation. Related Articles | Metrics

Select

Effects and mechanism of STAT3 inhibitor and nicotinamide combination on the inhibiting proliferation of hepatocarcinoma HepG2 cells ZHAN Wenjing, LIANG Mingjie, LIU Yuan, HUANG Yuanfeng, WANG Weixuan 2022, 50 (7):  686-692.  doi: 10.11958/20212609 Abstract ( 666 )   PDF (2636KB) ( 2204 )   Objective　To investigate the effects and underlying mechanisms of the combination of STAT3 inhibitors (Stattic and S3I-201) and nicotinamide adenine dinucleotide (NAD) precursor nicotinamide on the proliferation of hepatocarcinoma HepG2 cells. Methods　Hepatocarcinoma HepG2 cells were treated with STAT3 inhibitor, nicotinamide and their combination. Cell counting, Western blot assay and qPCR were used to examine the effects of drugs on cell proliferation, STAT3 expression and mRNA expression levels of STAT3 downstream target genes (SNAIL1, VEGFA and ZEB1), cell proliferation (MCM7, MKI67 and MYC), apoptosis (BCL-2, MCL-1 and BAX), epithelial-mesenchymal transformation (TJP1 and SMAD3), and glucose metabolism (HK2, PFKL, PKM, GLUT1 and LDHA)-related genes. Results　Both STAT3 inhibitor and nicotinamide treatment can decrease the phosphorylation level of STAT3, inhibit cell proliferation, downregulate the expression levels of genes related to cell proliferation, anti-apoptosis, EMT, and glucose metabolism, and upregulate the expression levels of genes related to pro-apoptosis. In addition, the combination of drugs showed a more significant effect on the phosphorylation level of STAT3, cell proliferation and expression levels of the above-mentioned cellular process-related genes. Conclusion　STAT3 inhibitor and nicotinamide can inhibit the proliferation of hepatocarcinoma cells, and the combined effect is more significant. This inhibition may be related to the promotion of apoptosis, the inhibition of EMT and glycolysis. Related Articles | Metrics

Select

Inhibitory effect of Rotigaptide on autonomic contraction of gastric smooth muscle in diabetic rats SUN Haibei, BAO Yitegele, ZHANG Mohan 2022, 50 (7):  693-697.  doi: 10.11958/20212756 Abstract ( 453 )   PDF (657KB) ( 2165 )   Objective　To discuss the effect and mechanism of Rotigaptide on the autonomic contractile movement of gastric smooth muscle in diabetic rats. Methods　The diabetic rat model (DM) was induced by streptozotocin (STZ). SD rats were divided into the control group and the DM group. In vitro muscle strip experiment was performed to observe changes of the autonomic contractile movement of the gastric smooth muscle before and after treatment with Rotigaptide in each group. Western blot assay was used to detect expression levels of Cx43 and PKCα in membrane protein and pulp protein, p-Cx43 Ser368 and p-PKCα Thr497 in gastric smooth muscle tissue of the control group, the DM group and the Rotigaptide group. Results　Compared with the control group, the amplitude and frequency of autonomic contraction in gastric smooth muscle contraction were decreased in the DM group (P＜0.05). In the control group, there were no changes in amplitude and frequency of autonomic contraction in gastric smooth muscle after treatment with Rotigaptide. In the DM group, after treatment with Rotigaptide, the amplitude and frequency of autonomic contraction in gastric smooth muscle contraction were significantly reduced (P＜0.05). Compared with the control group, the Cx43 content of membrane protein was increased, the Cx43 content of pulp protein was decreased, the membrane Cx43/pulp Cx43 ratio increased, and the p-Cx43 Ser368 content of membrane protein was increased in the DM group (P＜0.05). Compared with the DM group, the Cx43 content of membrane protein was decreased (P＜0.05), the Cx43 content of pulp protein was increased and the membrane Cx43/pulp Cx43 ratio was decreased in the Rotigaptide group (P＜0.05). Compared with the control group, the content of membrane protein PKCα was decreased, the content of pulp protein PKCα was increased and the membrane PKCα/pulp PKCα ratio was decreased in the DM group (P＜0.05). Compared with the DM group, the content of membrane protein p-PKCα Thr497 was increased in the Rotigaptide group (P＜0.05). Conclusion　Rotigaptide inhibits autonomic contraction of gastric smooth muscle in diabetic rats by down-regulating the number and the opening rate of gap junction by PKCα-Cx43 pathway activity. Related Articles | Metrics

Select

High-intensity focused ultrasound enhances cisplatin chemotherapy sensitivity of breast cancer through TRIF-mediated ERK pathway YI Wanping, MA Deshou 2022, 50 (7):  698-706.  doi: 10.11958/20212579 Abstract ( 482 )   PDF (1702KB) ( 2129 )   Objective　To explore the mechanism of high-intensity focused ultrasound (HIFU) enhancing the chemosensitivity of breast cancer to cisplatin (DDP) through TIR-domain-containing adaptor inducing interferon-β (TRIF)-mediated extracellular regulated protein kinase (ERK) pathway. Methods　Breast cancer cells (MDA-MB-231, MCF-7)/DDP-resistant cells were induced by intermittent action of increasing DDP concentration. The MDA-MB-231/DDP and MCF-7/DDP cells were divided into the control group, the HIFU group, the HIFU+Dimethyl sulfoxide (DMSO) group and the HIFU+fisetin group. CCK-8 was used to detect cell viability. Colony formation test was used to detect cell proliferation. Flow cytometry was used to detect cell cycle and apoptosis. Western blot assay was used to detect the expression of cell TRIF protein, drug resistance-related protein and ERK pathway protein. In vivo tumor formation test was used to detect tumor growth. Immunohistochemical method was used to detect the expression of Ki-67 in tumor tissues. Results　Compared with the parental MDA-MB-231 and MCF-7 cells, the MDA-MB-231/DDP and MCF-7/DDP cells had higher viability under the same concentration of DDP treatment, and the half inhibitory concentration (IC50) was significantly increased (P＜0.05), indicating the successful establishment of a stable anti-DPP drug-resistant cell line. Compared with the control group, the IC50, OD450 values of MDA-MB-231/DDP and MCF-7/DDP cells, number of cloned cells, percentage of S-phase cells, expression levels of B cell lymphoma-2 (Bcl-2), TRIF, p-glycoprotein (p-gp), multidrug resistance 1 (MDR1) and p-ERK1/2/ERK1/2 were significantly reduced in the HIFU group. The percentage of cells in G0/G1 phase, the apoptosis rate and the expression level of Bcl-2-associated X protein (Bax) were significantly increased (P＜0.05). Compared with the HIFU group, the IC50, OD450 values of MDA-MB-231/DDP and MCF-7/DDP cells, number of cloned cells, percentage of S-phase cells, expression levels of Bcl-2, TRIF, p-gp, MDR1 and p-ERK1/2/ERK1/2 were significantly increased in the HIFU+fisetin group, the percentage of cells in G0/G1 phase, the apoptosis rate, and the expression level of Bax were significantly reduced (P＜0.05). Compared with the control group, the tumor volume, tumor weight, the expression levels of TRIF and p-ERK1/2/ERK1/2, and the positive expression of Ki-67 were significantly reduced in the HIFU group (P＜0.05). Compared with the HIFU group, the tumor volume, tumor weight, expression levels of TRIF and p-ERK1/2/ERK1/2, and the positive expression of Ki-67 were significantly increased in the HIFU+fisetin group (P＜0.05). Conclusion　HIFU inhibits the ERK pathway mediated by TRIF expression, thereby enhancing the sensitivity of breast cancer DDP chemotherapy. Related Articles | Metrics

Select

Study on the mechanism of Fuzheng Huayu prescription drug-containing serum affecting the activation of activinA/smad signaling pathway in hepatic stellate cells CHEN Lixu, XIONG Jia, XIE Kun, ZHU Tingde, ZHONG Zhiying, GUAN Liang, PAN Yongping 2022, 50 (7):  707-712.  doi: 10.11958/20220030 Abstract ( 482 )   PDF (716KB) ( 2154 )   Objective　To investigate the effect of Fuzheng Huayu (Fzhy) Formula drug-containing serum on the activation of activinA/smad signaling pathway in hepatic stellate cells (HSC). Methods　Twenty SD rats were divided into the control group and the Fzhy-low, medium and high dose drug-containing serum group according to the random number table method, 5 rats in each group. Rats were gavaged with distilled water, 0.75, 1.5 and 3.0 g/kg Fzhy solution (drug powder of Fzhy capsule prepared with distilled water) once/d for 3 days, and blood samples were collected to prepare blank serum (the control group) and the drug-containing serum. HSC-T6 cells were cultured with 5%, 10% and 20% of the drug-containing serum in different volume fractions, respectively. Flow cytometry was used to detect cell cycle and apoptosis rate, mitochondrial membrane potential changes and reactive oxygen species (ROS) levels. Real-time fluorescence quantitative polymerase chain reaction was used to detect mRNA levels of activinA, smad3, samd7 and nuclear factor (NF)-κB in cells. Protein blotting was used to detect activin ⅡA receptor (ActRⅡA), smad3, NF-κB p65, and cysteine aspartate protease (caspase)-3 protein levels. Results　The cell survival rate of each Fzhy -containing serum group was lower than that of the control group (P＜0.05), and the drug-containing serum with a volume fraction of 10% was selected for subsequent experiments. There were no significant differences in cell cycle and apoptosis rates between the control group and each Fzhy-containing serum group. The proportion of intracellular ROS level and mitochondrial membrane potential level reduction increased in the control group and the Fzhy-low, medium and high dose groups successively (P＜0.05). Compared with the control group, smad3 mRNA expression level was decreased, and smad7 mRNA was increased in the Fzhy-medium and high dose groups. NF-κB and activinA mRNA, smad3, NF-κB p65 and ActRⅡA protein expression levels were decreased in the Fzhy-low and medium and high dose groups, and caspase-3 protein expression levels in the Fzhy-low and medium dose groups were increased (P＜0.05). Compared with the Fzhy-low dose group, smad3 mRNA expression levels were decreased in the Fzhy-medium and high dose groups, and activinA and smad7 mRNA were increased in the Fzhy-medium dose group (P＜0.05). Conclusion　Fzhy containing serum can achieve antifibrotic effects by affecting the proliferation of HSC-T6 cells, participating in cellular oxidative stress, and regulating cellular activinA/smad signaling pathways. Related Articles | Metrics

Select

Effects of electrical stimulation of vagus nerve on migration and differentiation of c-kit+ vascular wall cells SHI Liuliu, XU Zhen, WU Zhiyi, ZHAO Jinlong, LEI Jiaqi, WU Yan 2022, 50 (7):  713-718.  doi: 10.11958/20212823 Abstract ( 394 )   PDF (1177KB) ( 2195 )   Objective　To investigate the effect of electrical stimulation of vagus nerve on migration and differentiation of c-kit+ vascular wall cells in rats under carotid artery injury. Methods　A total of 24 SD rats were randomly divided into three groups: the sham-operation group, the operation group and the vagus nerve stimulation group, with 8 rats in each group. After the common carotid artery balloon injury model was carried out, the left vagus nerve was electrically stimulated in the vagus nerve stimulation group. HE staining and immunohistochemical staining were used to observe the thickness of vascular intima and the distribution of c-kit+ vascular wall cells. The serum levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA). After isolating c-kit+ cells from the vessel wall, the c-kit+ cells were divided into three groups：the control group (no acetylcholine), the 10-5 mol/L acetylcholine group and the 10-5 mol/L acetylcholine group with the + alpha 7 nicotinic acetylcholine receptor (α7nAChR) agonist group. Transwell assay was applied to detect the effect of acetylcholine on c-kit+ migration, and the expression of smooth muscle 22 alpha (SM22α) was detected by Western blot assay. Results　(1) Compared with operation group, the thickness of vascular neointima was reduced, and the number of c-kit+ cells in vascular neointima was decreased in the vagus nerve stimulation group (P＜0.05), and the serum levels of TNF-α and IL-6 were also reduced (P＜0.01). (2) Acetylcholine inhibited the c-kit+ migration and increased the expression of SM22α, which could be reversed by α7nAChR agonist. Conclusion　Acetylcholine can mitigate the neointimal formation through inhibiting the migration of c-kit+ and accelerating the differentiation of c-kit+ to vascular smooth muscle cells, the mechanism of which may be related to acetylcholine combined with α7nAChR that activating anti-inflammatory system. Related Articles | Metrics

Select

Effects of dapagliflozin on cardiac function and myocardial remodeling in rabbits with non-diabetic chronic heart failure CHEN Xuefeng, ZHAO Ming, ZHANG Yu, HAO Ziqiang, BAI Wenlou, MENG Cunliang, QI Xiaoyong 2022, 50 (7):  719-725.  doi: 10.11958/20220040 Abstract ( 627 )   PDF (905KB) ( 2229 )   Objective　To investigate the effects of dapagliflozin on cardiac function and myocardial remodeling in rabbits with non-diabetic chronic heart failure. Methods　A total of 18 male New Zealand white rabbits were randomly divided into the sham group, the heart failure group and the dapagliflozin group, with 6 rabbits in each group. The sham group only performed thoracotomy without operation, and the heart failure group and the dapagliflozin group were treated with aortic constriction after thoracotomy. Nondiabetic chronic heart failure model was established after 12 weeks. The general situation was observed in each group. The dapagliflozin group was given dapagliflozin 1 mg/ (kg·d) by force-feeding method 13 weeks after operation, and the sham group and the heart failure group were given the same amount of normal saline for a total of 10 weeks. Echocardiography was performed before surgery, 12 weeks after surgery and 10 weeks after drug intervention. Body weight, white blood cell (WBC) count, total protein, albumin, hemoglobin, potassium, sodium, random blood glucose, osmotic pressure and NT-proBNP were detected after 10 weeks of drug intervention. After the animals were sacrificed, whole heart and left ventricle mass were measured, the morphology and fibrosis degree of myocardial tissue were observed by HE, Masson and immunohistochemistry staining, and collagen tissue fraction and positive percentage of Collagen Ⅰ or Ⅲ area were calculated. Results　Dapagliflozin ameliorated symptoms such as loss of appetite, lethargy, reduced activity and tachypnea in animals with heart failure. After 10 weeks of drug intervention, the left ventricular ejection fraction (LVEF) was significantly higher in the dapagliflozin group than that in the heart failure group, and significantly higher than that of 12 weeks after operation (P＜0.05). There were no significant differences in levels of WBC, hemoglobin, total protein, albumin, potassium, sodium, blood glucose and osmotic pressure between the three groups. The hemoglobin concentration were lower in the dapagliflozin group and the heart failure group compared with that of the sham group, but there was no significant difference between the dapagliflozin group and the heart failure group. Compared with the heart failure group, the heart shape of dapagliflozin group was decreased, and the total heart mass, left ventricular mass and left ventricular/body mass of the dapagliflozin group were lower than those of the heart failure group (P＜0.05). HE staining showed that dapagliflozin could significantly improve the morphological changes of myocardial cells. Masson staining showed that collagen fiber fraction in myocardial tissue was significantly reduced in the dapagliflozin group compared with that of the heart failure group (P＜0.05). Immunohistochemical analysis showed that the percentage of positive Collagen Ⅰ and Collagen Ⅲ area in myocardial tissue of the dapagliflozin group was lower than that of the heart failure group (P＜0.05). Conclusion　Dapagliflozin inhibits cardiac remodeling after heart failure, the mechanism of which may be related to the inhibition of Collagen Ⅰ and Ⅲ expression in myocardial tissue and myocardial fibrosis. Related Articles | Metrics

Select

Vertical changes of extraction cases with clear aligners and miniscrews LIU Weitao, SUN Jia, QI Lin, ZOU Bingshuang, ZHOU Shaonan, ZHOU Yanheng 2022, 50 (7):  726-733.  doi: 10.11958/20220702 Abstract ( 521 )   PDF (1452KB) ( 2100 )   Objective　To explore dentoskeletal changes and factors that correlated with vertical control effect in extraction cases treated with clear aligner appliances and miniscrews. Methods　Thirty-two patients (mean age, 27.1±4.3 years), who were extracted 4 premolars followed by Invisalign treatement with upper miniscrews for retraction of anterior teeth, were retrospectively selected. Cone-beam computed tomography (CBCT) images were taken before and after treatment. Treatment changes in the position of the anterior and posterior teeth, menton, mandibular plane angle (MP-SN), occlusal plane angle (OP-SN) and lower face height (ANS-Me) were measured on the CBCT and cephalometric radiographs generated from CBCT and counted using paired-comparison test. The correlations of significant variables with mandibular plane angle were evaluated using correlation analysis. Results　After treatment, the upper incisors showed significant retraction (6.76±1.75) mm and intrusion (1.57±1.54) mm, and the lower incisors were also significantly retracted (5.56±1.37) mm and intruded (2.94±1.15) mm. The mean vertical change in upper molars was a (1.21±0.79) mm intrusion, while a (0.36±0.92) mm extrusion in lower. Regarding the anteroposterior (AP) dimension, there was a significantly mesial movement in both upper (1.65±1.21 mm) and lower (1.30±0.84 mm) molars. The mean changes in mandibular plane angle was a 1.10°±0.99° of counterclockwise rotation with a anterosuperior movement in menton and a decrease in lower face height, whereas the occlusal plane angle was clockwise rotated. The changes above were all statistically significant (P＜0.05). The changes of mandibular plane angle was significantly correlated with the vertical position of lower molars (r=0.731，P＜0.01), upper molars (r=-0.485，P＜0.01) and upper incisors (r=-0.380, P＜0.05), the AP position of lower incisors (r=0.405，P＜0.05), and the changes of OP-SN angle (r=0.437，P＜0.05). Conclusion　Except retracted upper anterior teeth, extraction cases treated with Invisalign and upper miniscrews appear to have a relatively good vertical control with a decreased mandibular plane angle, which might be associated with the vertical position of upper and lower molars, vertical position of upper incisors, and the AP position of lower incisors. Related Articles | Metrics

Select

Correlation between myeloid-derived suppressor cells and peripheral blood monocytes DU Weijiao, ZHANG Jiali, YU Wenwen, SHEN Meng, CAO Shui 2022, 50 (7):  734-738.  doi: 10.11958/20212634 Abstract ( 504 )   PDF (517KB) ( 2145 )   Objective　To investigate the correlation between tumor infiltrating myeloid-derived suppressor cells (MDSCs) and peripheral blood leukocytes in patients with breast cancer, and its effect on the relapse-free survival (RFS). Methods　The clinical data and the proportion of tumor infiltrating MDSCs of 32 patients with breast cancer who underwent radical surgery in Tianjin Cancer Hospital were collected. The relationship between MDSCs and clinicopathological features was analyzed. The correlation between MDSCs and peripheral blood leukocytes was observed by Spearman correlation analysis. The patients were divided into the high MDSCs group (≥4.4%, n=16) and the low MDSCs group (＜4.4%, n=16) according to the median MDSCs. The difference of RFS between the two groups was analyzed. Results　The proportion of tumor infiltrating MDSCs was higher in patients with stage Ⅲ than that of patients with stageⅠ-Ⅱ (P＜0.05). The proportion of MDSCs infiltrated by tumor was positively correlated with peripheral blood monocyte count (rs=0.405) and monocyte to lymphocyte ratio (MLR) (rs=0.408, P＜0.05). Overall analysis results showed that RFS values of the high and low MDSCs groups were [(102.7±9.1) months and (107.9±8.3) months], and there was no significant difference between the two groups (Log-rank χ2=1.166, P＞0.05). Stratified analysis showed that in 17 cases without lymph node metastasis RFS was significantly shorter in the high MDSC group [(97.4±31.9) months] than that of the low MDSCs group [(117.8±15.9) months, Log-rank χ2=5.402, P＜0.05]. Conclusion　The proportion of MDSCs infiltrating in breast cancer is positively correlated with peripheral blood monocyte count and MLR. Patients without lymph node metastasis have worse RFS in the high MDSCs group. Related Articles | Metrics

Select

Study on the load of non-criminal plaques in NSTE-ACS patients with type 2 diabetes CHEN Meng, ZHANG Ying, LIU Yujie 2022, 50 (7):  739-743.  doi: 10.11958/20212423 Abstract ( 398 )   PDF (403KB) ( 2146 )   Objective　To quantitatively evaluate the plaque load of non-criminal lesions (NCL) by coronary CT angiography (CTA) in patients with non-ST-segment elevation acute coronary syndrome (NSTE-ACS) complicated with type 2 diabetes mellitus (T2DM). Methods　A total of 112 patients with NSTE-ACS who underwent coronary CTA examination were divided into the experimental group (58 cases) and the control group (54 cases) according to whether they were complicated with T2DM. Patients in both groups were successfully treated with PCI for criminal lesions (CL) shown by coronary angiography (CAG). Semi-automatic plaque quantification software was used to assess NCL plaque load in the two groups. Results　The non-calcified plaque volume, low-density non-calcified plaque volume, total plaque volume, non-calcified plaque load, low-density non-calcified plaque load and total plaque load of NCL plaques were significantly higher in the experimental group than those in the control group (P＜0.01). There were no significant differences in NCL plaque volume, calcified plaque load and RI between the two groups (P＞0.05). There was a positive correlation between blood glucose and NCL plaque non-calcified plaque volume (rs=0.472), low-density non-calcified plaque volume (rs=0.370), total plaque volume (rs=0.522), non-calcified plaque load (rs=0.502), low density non-calcified plaque load (rs=0.536) and total plaque load (rs=0.486). Conclusion　Patients with NSTE-ACS combined with T2DM have a large NCL plaque load, and blood glucose affects the plaque volume and load. Related Articles | Metrics

Select

Efficacy and experience of five-step low power direct- release green laser prostatic enucleation in the treatment of large prostatic hyperplasia WEN Zhu, ZHANG Xiansheng 2022, 50 (7):  743-747.  doi: 10.11958/20212774 Abstract ( 776 )   PDF (498KB) ( 2132 )   Objective　To explore clinical effectiveness and safety of five-step method-low power end-fire greenlight laser in transurethral vaporresection-enucleation of prostate (VREP) for the treatment of begnin prostatic hyperplasia (BPH). Methods　Data of 71 patients with BPH who received treatment at Binhu Hospital of Hefei from January 2019 to September 2020 were retrospectively analyzed. Among them, 38 patients were treated with five-step method (the VREP group), and the other 33 patients were treated with photoselective vaporization of prostate (the PVP group). In the VREP group, the operation was performed by a five-step method of locating, grooving, dividing, enucleation and crushing, while in the PVP group, the entire gland was uniformly vaporized layer by layer with 180W power. Perioperative data and complications were recorded and analyzed. Changes in IPSS, QOL, Qmax, PVR, PSA and other indicators before and after surgery were compared between the two groups. Results　All patients were successfully operated without capsular perforation, water poisoning and other complications. The average operation time was ［56.00 (50.75, 59.00) min in the VEP group and 65.00 (57.00, 71.50) min］ in the PVP group (P＜0.01). Values of hemoglobin decreased after operation in the VEP group and the PVP group ［17.00 (12.00, 23.25) g/L and 14.00 (9.50, 20.50) g/L］. The time of indwelling catheterization was ［2.00 (2.00, 2.00) d vs. 2.00 (2.00, 2.00) d］for the two groups, and the hospitalization time was［3.00 (3.00, 4.00) d vs. 3.00 (3.00, 4.00) d］ (P＞0.05). PVR, Qmax, IPSS, QOL and PSA improved significantly 3 months after operation. IPSS［9.00 (7.00,10.00) vs. 10.0 (9.00, 11.50)］and PSA ［3.08 (1.33, 5.98) μg/L vs. 5.05 (3.05, 6.72) μg/L］were significant lower in the VREP group than those of the PVP group (P＜0.05). Conclusion　Five-step method low power end-fire greenlight laser VREP treatment of BPH has the advantages of short operation time, higher effective gland clearance rate and fast postoperative recovery, which can be regarded as a safe and effective surgical method and is worth promoting. Related Articles | Metrics

Select

Clinical study of painless gastroscopy using abdominal pressure lifting apparatus in overweight and obese patients WU Yuanyuan, MA Xiangti, XIE Liping 2022, 50 (7):  748-753.  doi: 10.11958/20220048 Abstract ( 853 )   PDF (459KB) ( 2127 )   Objective　To explore the effect and mechanism of abdominal pressure lifting apparatus on painless gastroscopy in overweight and obese patients. Methods　A total of 210 overweight and obese patients who underwent painless gastroscopy were selected and divided into the experimental group (n=105) and the control group (n=105) according to random number table method. Before the examination, patients were given pure oxygen at a flow rate of 5 L/min for 3 min, and then slowly injected remifentanil 0.25 μg/kg and propofol 2 mg/kg successively. Gastroscopy was performed when the modified observer’s assessment of alert /sedation (MOAA/S) was ≤3. Abdominal lifting and pressing were carried out for 10 times in the experimental group at the time of 90 s after anesthesia, while no intervention was performed in the control group. Basic vital signs of patients were monitored throughout the whole process. Data of systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate (HR) and oxygen saturation of finger pulse (SpO2) were recorded and compared when the patients entered the room (T0), the eyelash reflex disappeared (T1), the time of 90 s after anesthesia (T2), at the end of examination (T3), at the time of recovery from anesthesia (T4) and at the time of leaving the clinic (T5). The incidence and degree of respiratory related adverse events were recorded and compared between the two groups, and drug dosage, examination time, anesthesia time, awakening time, independent sitting time, leaving time and the occurrence of adverse events were also recorded and compared. Results　At the time of T0, there were no significant differences in HR, SBP, DBP and SpO2 between the two groups. At the time points of T0 to T5, there were no significant differences in SBP and HR between the two groups. At the time points of T1 and T4, DBP was significantly lower in the experimental group than that of the control group (P＜0.05). At time points of T3 and T4, the SpO2 was significantly higher in the experimental group than that of the control group (P＜0.05). There were no significant differences in the general information, drug dosage, anesthesia time, examination time, awakening time and leaving time between the two groups. However, the independent sitting time was significantly shorter in the experimental group than that of the control group (P＜0.05). The proportion of patients with subclinical respiratory depression was significantly higher in the experimental group than that in the control group, but the proportion of patients with hypoxia, severe hypoxia, mandibular support and mask pressure oxygen supply was lower in the experimental group than that in the control group (P＜0.05). There was no significant difference in the occurrence of adverse reactions between the two groups (P＞0.05). Conclusion　The use of abdominal pressure lifting apparatus during painless gastroscopy in overweight and obese patients reduces the overall incidence of respiratory adverse events and the number of respiratory interventions. Related Articles | Metrics

Select

Predictive value of total cholesterol level in the early cardiac insufficiency in patients undergoing off-pump coronary artery bypass grafting ZHANG Haitao, WEI Wei, WANG Lei, LI Yan, FENG Zengbin 2022, 50 (7):  753-756.  doi: 10.11958/20212389 Abstract ( 466 )   PDF (397KB) ( 2103 )   Objective　To investigate the correlation between total cholesterol (TC) levels and the development of the early cardiac insufficiency in patients after off-pump coronary artery bypass grafting (OPCAB). Methods　A total of 201 patients who underwent OPCAB were retrospectively analyzed. Patients were divided into the cardiac insufficiency group (with the occurrence of early postoperative cardiac insufficiency, n=22) and the control group (without early postoperative cardiac insufficiency, n=179) according to the diagnostic criteria of early postoperative cardiac insufficiency. The laboratory and clinical data were collected. The preoperative clinical baseline data were compared between the 2 groups. The relevant study factors with statistically significant differences were included in a multifactorial dichotomous Logistic regression analysis to analyze the factors influencing the occurrence of the early postoperative cardiac insufficiency in patients after OPCAB. The predictive value of influencing factors was analyzed using the subject work characteristic (ROC) curve. Results　There were no significant differences in age, gender, body mass index (BMI), glucose, systolic blood pressure, preoperative left ventricular ejection fraction (LVEF), number of transplanted vessels, smoking, history of myocardial infarction and history of cerebral infarction between the cardiac insufficiency group and the control group (P＞0.05), and there were significant differences in baseline TC, triacylglycerol (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) levels between the two groups (P＜0.05). The results of dichotomous Logistic regression analysis showed that the elevated TC level was a risk factor for the development of early postoperative cardiac insufficiency in OPCAB patients (OR=11.842, 95%CI: 4.055-34.580, P＜0.01). The area under the ROC curve AUC was 0.974 (95%CI: 0.954-0.994). The sensitivity was 0.955 and specificity was 0.922. Conclusion　Serum TC level can effectively predict the occurrence of early cardiac insufficiency in patients undergoing OPCAB, and the higher the serum TC level, the greater the likelihood of early postoperative cardiac insufficiency. Related Articles | Metrics

Select

Correlation between miR-155 and PDCD4 expression in peripheral blood of infants with respiratory syncytial virus bronchiolitis WANG Gang, WANG Qiong, JIANG Xin, ZHENG Jing 2022, 50 (7):  757-761.  doi: 10.11958/20220359 Abstract ( 423 )   PDF (414KB) ( 2109 )   Objective　To explore the relationship between the expression of miR-155 and programmed cell death 4 (PDCD4) in peripheral blood of infants with respiratory syncytial virus (RSV) bronchiolitis. Methods　A total of 106 infants and young children with RSV bronchiolitis accepted by our hospital were collected as the research group. According to the severity of the disease, patients were separated into the mild group (n=36), the moderate group (n=41), and the severe group (n=29). Sixty-five infants who underwent physical examination in the child care clinic of our hospital were selected as the control group (n=55). The serum level of miR-155 in peripheral blood of infants and young children was measured by real-time quantitative PCR (qPCR) method. Levels of PDCD4, high sensitivity C-reactive protein (hs-CRP), interleukin (IL)-6, IL-10 and tumor necrosis factor (TNF)-α were measured by enzyme-linked immunosorbent assay (ELISA). The relationship between serum miR-155 and PDCD4 and their correlation with inflammatory factors in infants with RSV bronchiolitis were analyzed by Pearson correlation. Results　Compared with the control group, the serum level of miR-155 was significantly increased in the study group, and the level of PDCD4 was significantly decreased (P＜0.05). In addition, the serum miR-155 level increased with the aggravation of the severity of RSV bronchiolitis, and the PDCD4 level reduced significantly (P＜0.05). Compared with the control group, serum levels of inflammatory factors hs-CRP, IL-6, IL-10 and TNF-α increased significantly in the study group (P＜0.05). Pearson correlation analysis showed that miR-155 and PDCD4 were negatively correlated (r=-0.790, P＜0.01), miR-155 was positively correlated with hs-CRP, IL-6, IL-10, and TNF-α (P＜0.01), and PDCD4 was negatively correlated with hs-CRP, IL-6, IL-10, and TNF-α (P＜0.01). Conclusion　RSV bronchiolitis in infants and young children has abnormal expression of serum levels of miR-155 and PDCD4, and the two levels are negatively correlated. Serum levels of miR-155 and PDCD4 are tightly related to inflammatory factors. Related Articles | Metrics

Select

Comparison of clinical effect of thoracic drainage microtube and thoracoscopy in treatment of emphysema complicated with giant pulmonary bullae CHEN Jian, YANG Li, JIN Jie, WANG Lei, DONG Min, CHAI Guoxiang 2022, 50 (7):  761-765.  doi: 10.11958/20212631 Abstract ( 455 )   PDF (608KB) ( 2116 )   Objective　To compare the clinical efficacy of chest drainage microtubules and lung volume reduction surgery (LVRS) in the treatment of emphysema complicated with giant bullae. Methods　A total of 88 patients with emphysema complicated with giant bullae were selected as subjects. Patients were divided into the microtubule group (49 cases) and the LVRS group (39 cases) according to the treatment method. The differences in operative time, blood loss and postoperative hospital stay were compared between the two groups. Pulmonary function [percentage of forced end-expiratory volume in 1 second to estimated value (FEV1% estimated value) and FEV1 to forced vital capacity (FEV1/FVC)], pulmonary artery pressure, arterial partial pressure of oxygen [p(O2)], partial pressure of carbon dioxide [p(CO2)] and oxygen saturation before surgery were recorded before surgery, 2 weeks and 2 months after surgery. Postoperative dyspnea index and quality of life score were evaluated. Results　Compared with the LVRS group, there were shorter operation time, less blood loss and shorter postoperative hospital stay in the microtubule group (P＜0.01). Compared with before surgery, FEV1% predicted value, FEV1/FVC, p(O2) and blood oxygen saturation were increased 2 months after surgery in the two groups, while pulmonary artery pressure and p(CO2) were decreased, and the above indexes were significantly improved in the microtubule group compared with those of the LVRS group (P＜0.05). There was no significant difference in postoperative arterial oxygen saturation between the two groups (P＞0.05). Two months after operation, the proportion of dyspnea index Ⅲ to Ⅳ was lower in the microtubule group than that in the LVRS group, and the quality of life score was higher in the microtubule group (P＜0.05). Conclusion　For patients with emphysema complicated with giant bullae, thoracic drainage microtubules have less trauma and faster recovery of lung function and blood oxygen after operation, which is a good clinical treatment. Related Articles | Metrics

Select

The serum levels and their clinical significance of LDLR and DNM2 in elderly patients with chronic heart failure LI Chunxia, YANG Zhiming, LI Jun, BU Xingpeng 2022, 50 (7):  766-770.  doi: 10.11958/20212843 Abstract ( 587 )   PDF (439KB) ( 2109 )   Objective　To investigate the serum levels of low density lipoprotein receptor (LDLR) and dynamin 2 (DNM2) in elderly patients with chronic heart failure (CHF) and their clinical significance. Methods　A total of 123 CHF patients (the CHF group) and 120 healthy people (the control group) were selected. According to New York Heart Association (NYHA) classification, there were 42 cases of grade Ⅱ, 61 cases of grade Ⅲ and 20 cases of grade Ⅳ in the CHF group. The serum levels of LDLR, DNM2 and cardiac function indexes were compared between the CHF group and the control group. Pearson correlation analysis was performed to analyze the correlation between LDLR, DNM2, left ventricular end-diastolic diameter (LVEDD), left ventricular ejection fraction (LVEF) and N-terminal B-type brain natriuretic peptide precursor (NT-probNP). After six months of follow-up, CHF patients were divided into the good prognosis group (98 cases) and the poor prognosis group (25 cases). The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of LDLR and DNM2 for CHF with poor prognosis. Results　Compared with the control group, the serum levels of LDLR, DNM2 and LVEF decreased in patients with different NYHA grades than those of the control group, while the levels of LVEDD and NT-proBNP were higher than those of the control group (P＜0.05). With the increase of NYHA classification, serum levels of LDLR, DNM2 and LVEF gradually decreased in CHF patients, while LVEDD and NT-proBNP levels gradually increased (P＜0.05). Serum levels of LDLR and DNM2 were positively correlated with LVEF in CHF patients, but negatively correlated with LVEDD and NT-proBNP (P＜0.01). The serum levels of LDLR and DNM2 were lower in the poor prognosis group than those in the good prognosis group (P＜0.05). The ROC curve showed that the area under the curve of LDLR combined with DNM2 in the diagnosis of poor prognosis in CHF patients was significantly higher [0.858 (95%CI: 0.751-0.965)] than that of LDLR [0.793 (95%CI: 0.668-0.919)] and DNM2 [0.777 (95%CI: 0.669-0.884)], the sensitivity and specificity were improved. Conclusion　Serum levels of LDLR and DNM2 are decreased with the the increase of NYHA classification in CHF patients, which can reflect cardiac function. The combination of the two indicators is helpful to evaluate the prognosis of patients with CHF. Related Articles | Metrics

Select

Effects of ticagrelor on platelet function in patients with minor acute stroke or TIA GONG Lei, SUN Haiying, JIANG Xianfeng, GONG Ping, YANG Xuejun 2022, 50 (7):  771-774.  doi: 10.11958/20211970 Abstract ( 515 )   PDF (372KB) ( 2091 )   Objective　To explore the effect of ticagrelor on platelet function (PF) in patients with minor acute stroke or transient ischemic attack (TIA). Methods　A total of 81 minor acute stroke or TIA patients were randomly divided into the experimental group (39 patients) and the control group (42 patients). The experimental group was treated with ticagrelor combined with aspirin after admission, and changed to ticagrelor alone after 21 days. The control group was treated with clopidogrel after clopidogrel and aspirin treating for 21 days. On admission, day 7 and day 90 Sonoclot assay was used to measure PF in the two groups. Results　(1) Compared with the control group, the values of activated clotting time (ACT) and time to peak (PT) increased on the 7th day of treatment in the experimental group, and PF decreased. ACT also increased 90 days after treatment (P＜0.05). There were no significant differences in clot rate (CR) and maximal clot signal (MCS) before and after treatment in the two groups (P＞0.05). (2) Compared with those at admission, the values of ACT and TP increased and the PF and MCS decreased in the two groups (P＜0.05). (3) There were no significant differences in the main adverse events between the two groups (P＞0.05). Conclusion　Compared with clopidogrel, ticagrelor can inhibit platelet function more rapidly in patients with minor acute stroke or TIA, but does not increase major bleeding events. Related Articles | Metrics

Select

Effects of dezocine combined with different loading dose of dexmedetomidine on awake fiberoptic nasotracheal intubation in patients with obstructive sleep apnea-hypopnea syndrome CUI Songqin, HUANG Hongfang, HUANG Xuelian, RUI Xiao, HUANG Saisai 2022, 50 (7):  775-779.  doi: 10.11958/20212855 Abstract ( 457 )   PDF (403KB) ( 2112 )   Objective　To observe the efficacy of dezocine combined with different loading dose of dexmedetomidine on awake fiberoptic nasotracheal intubation (AFNI) guided by fiberoptic bronchoscopy in patients with obstructive sleep apnea-hypopnea syndrome (OSAHS). Methods　Eighty patients with OSAHS were randomly assigned into 4 groups：the dezocine group (group C)，the dezocine 0.1 mg/kg combined with DEX 0.5 μg/kg group (group D1)，the dezocine 0.1 mg/kg combined with DEX 1 μg/kg group (group D2) and the dezocine 0.1 mg/kg combined with DEX 1.5 μg/kg group (group D3), 20 cases in each group．All patients were given 0.1 mg/kg of dezocine intravenously. The same amount of normal saline was intravenously pumped in group C, and DEX was infused over 10 min by micro-injection pump (group D1: 0.5 μg/kg，group D2: 1 μg/kg，group D3: 1.5 μg/kg) before intubation respectively. DEX 0.5, 1 and 1.5 µg/kg were intravenously pumped in group D1, group D2 and group D3, respectively. Naso-pharyngeal and laryngeal mucous membrane were sprayed with 2% lidocaine for topical anesthesia 5 min before intubation in the four groups. AFNI was performed after the end of administration in the four groups. The heart rate (HR)，mean arterial pressure (MAP)，oxygen saturation (SPO2)，the value of BIS and Ramsay sedation score at entering the OR (T0)，the end of administration (T1) and after intubation (T2) were observed between the four groups. Intubation tolerance, intubation time, patient satisfaction, airway obstruction score and incidence of adverse reactions (bradycardia, respiratory depression, agitation, memory of intubation) were also compared between the four groups. Results　Repeated-measures ANOVA showed that there were interaction between time factor and different intervenient measures on HR, MAP, BIS value and Ramsay sedation score during the experiment (P＜0.05), while there was no interaction with SPO2 (P＞0.05), only time factor affected SPO2 (P＜0.05). Further comparison between the four groups showed that HR and MAP were lower at T1 and T2 in the group D2 than those of the group C (P＜0.05), while HR and MAP were lower at T2 in the group D2 than those in the group D1 (P＜0.05). Compared with the group C and the group D1, the HR and MAP were lower at T1 and T2 in the group D3 (P＜0.05). HR and MAP were lower at T1 and T2 in the D3 group than those in the C group and the D1 group. The incidence of bradycardia was higher in the group D3 than that in the group C (P＜0.05). Compared with the group C and the group D1, the BIS values were lower at T1 and T2, and the Ramsay sedation scores were increased in the group D2 and the group D3 (P＜0.05). The incidence of dysphoria and the remembering of the AFNI, the intubation time were decreased, but the tolerance of AFNI and the patients’ satisfaction with the AFNI were increased in the group D2 and the group D3 than those in the group C and the group D1 (P＜0.05). Conclusion　Dezocine combined with DEX can effectively and safely used for AFNI in patients with OSAHS. In addition, dezocine 0.1 mg/kg combined with DEX 1 μg/kg can not only maintain hemodynamic stability of patients but also reduce the incidence of adverse reactions. Related Articles | Metrics

Select

Research progress of circular RNA in diabetic kidney disease JIANG He, LIU Wu, LYU Chunyi, ZHANG Zhao 2022, 50 (7):  780-784.  doi: 10.11958/20212810 Abstract ( 496 )   PDF (367KB) ( 2104 )   Diabetic kidney disease (DKD) is one of the most common complications of diabetes mellitus with a complex pathological process that often involves the entire renal structure. Circular RNAs are non-coding RNAs with a covalently closed loop structure, which play a role in various physiological and pathological processes. During DKD, circular RNAs are involved in renal cell injury by participating in the regulation of cell cycle, cell differentiation and death, and regulating processes such as inflammatory factor release and stromal fibronectin expression. In this paper, we introduce the structure and function of circular RNAs and review the recent research progress of circular RNAs in DKD from the perspective of renal cell injury. Related Articles | Metrics