Tianjin Medical Journal

Tianjin Medical Journal ›› 2022, Vol. 50 ›› Issue (7): 757-761.doi: 10.11958/20220359

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Correlation between miR-155 and PDCD4 expression in peripheral blood of infants with respiratory syncytial virus bronchiolitis

WANG Gang, WANG Qiong, JIANG Xin, ZHENG Jing   

  1. 1 Department of Pediatrics, Shanghai Pubin Children's Hospital, Shanghai 200120, China; 2 Dabaidoc Pediatrics, Shanghai Quyang Hospital; 3 Department of Immunology and Pathogen Biology, Shanghai University of Traditional Chinese Medicine; 4 Department of Biochemistry, Shanghai University of Traditional Chinese Medicine
  • Received:2022-03-09 Revised:2022-04-08 Published:2022-07-15 Online:2022-07-15

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Abstract: Objective　To explore the relationship between the expression of miR-155 and programmed cell death 4 (PDCD4) in peripheral blood of infants with respiratory syncytial virus (RSV) bronchiolitis. Methods　A total of 106 infants and young children with RSV bronchiolitis accepted by our hospital were collected as the research group. According to the severity of the disease, patients were separated into the mild group (n=36), the moderate group (n=41), and the severe group (n=29). Sixty-five infants who underwent physical examination in the child care clinic of our hospital were selected as the control group (n=55). The serum level of miR-155 in peripheral blood of infants and young children was measured by real-time quantitative PCR (qPCR) method. Levels of PDCD4, high sensitivity C-reactive protein (hs-CRP), interleukin (IL)-6, IL-10 and tumor necrosis factor (TNF)-α were measured by enzyme-linked immunosorbent assay (ELISA). The relationship between serum miR-155 and PDCD4 and their correlation with inflammatory factors in infants with RSV bronchiolitis were analyzed by Pearson correlation. Results　Compared with the control group, the serum level of miR-155 was significantly increased in the study group, and the level of PDCD4 was significantly decreased (P＜0.05). In addition, the serum miR-155 level increased with the aggravation of the severity of RSV bronchiolitis, and the PDCD4 level reduced significantly (P＜0.05). Compared with the control group, serum levels of inflammatory factors hs-CRP, IL-6, IL-10 and TNF-α increased significantly in the study group (P＜0.05). Pearson correlation analysis showed that miR-155 and PDCD4 were negatively correlated (r=-0.790, P＜0.01), miR-155 was positively correlated with hs-CRP, IL-6, IL-10, and TNF-α (P＜0.01), and PDCD4 was negatively correlated with hs-CRP, IL-6, IL-10, and TNF-α (P＜0.01). Conclusion　RSV bronchiolitis in infants and young children has abnormal expression of serum levels of miR-155 and PDCD4, and the two levels are negatively correlated. Serum levels of miR-155 and PDCD4 are tightly related to inflammatory factors.

Key words: respiratory syncytial viruses, bronchiolitis, viral, miR-155, programmed cell death factor 4, infants and young children