天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (5): 589-593.doi: 10.11958/20150212

• 实验研究 • 上一篇    下一篇

五味子提取物对百草枯中毒模型小鼠肺纤维化的保护作用

肖娜 1， 曹波 2， 刘斌 3， 王火 3， 高颖 2， 姚立夫 2， 魏路清 3△   

  1. 1辽宁医学院中国人民武装警察部队后勤学院附属医院研究生培养基地（邮编 300162）； 2中国人民武装警察部队后勤学院； 3中国人民武装警察部队后勤学院附属医院呼吸与危重症医学科
  • 收稿日期:2015-10-10 修回日期:2015-12-29 出版日期:2016-05-15 发布日期:2016-05-18
  • 通讯作者: △通讯作者 E-mail:luqing-wei@163.com E-mail:583078417@qq.com
  • 作者简介:肖娜 （1988）， 女， 硕士在读， 主要从事急诊与呼吸危重症研究
  • 基金资助:
    天津市应用基础与前沿技术研究计划青年项目 （14JCQNJC10300）； 武警后勤学院院级科学基金 （WHM201308）

Protection of Schisandra chinensis extract in paraquat-induced pulmonary fibrosis in mice

XIAO Na1, CAO Bo2, LIU Bin3, WANG Huo3, GAO Ying2, YAO Lifu2, WEI Luqing3△   

  1. 1 the Affiliated Hospital of Logistics University of Chinese Armed Police Forces， Postgraduate Training Base of Liaoning Medical University, Tianjin 300162, China; 2 Logistics University of Chinese Armed Police Forces; 3 Department of Respiratory and Critical Care Medicine, the Affiliated Hospital of Logistics University of Chinese Armed Police Forces
  • Received:2015-10-10 Revised:2015-12-29 Published:2016-05-15 Online:2016-05-18
  • Contact: △Corresponding Author E-mail:luqing-wei@163.com E-mail:583078417@qq.com

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摘要： 摘要： 目的 探讨五味子提取物对百草枯（PQ）中毒模型小鼠肺纤维化的保护作用， 并探讨其机制。方法 108 只小鼠按随机数字表法分为正常对照组， 模型组， 五味子提取物低剂量组 （200 mg/kg）、 中剂量组 （400 mg/kg）、 高剂量组 （800 mg/kg） 及维生素 C 组 （100 mg/kg）， 每组 18 只。除正常对照组以外的小鼠行一次性 PQ 溶液 （100 mg/kg） 灌胃造模， 造模成功后每 24 h 给予相应剂量药物 1 次， 分别在建模后第 7、 14、 21 天处死小鼠， 每个时间点 6 只。解剖肺脏， HE 染色观察小鼠肺组织炎症程度并进行炎症评分； Masson 染色观察肺组织纤维化程度；RT-PCR 和酶联免疫吸附试验（ELISA）分别检测各组肺组织中转化生长因子（TGF） -β1、 白细胞介素（IL） -6、 IL-17 mRNA 及蛋白表达水平。结果 （1）造模后第 7 天和第 14 天， 模型组小鼠肺组织出现大量炎性细胞浸润、 肺泡间隔充血， 炎症评分较正常对照组升高（P < 0.05）， 而五味子提取物中、 高剂量组小鼠肺组织炎症评分较模型组和维生素 C 组降低（P < 0.05）。（2）造模后第 14 天和第 21 天， 五味子中、 高剂量组小鼠肺组织纤维化程度较模型组降低（P < 0.05）。（3）随着造模时间的延长， 模型组 TGF-β1 mRNA 及蛋白表达水平升高， IL-6 表达降低， IL-17 则先升高后降低。五味子提取物中、 高剂量组与模型组比较， 造模后第 7 天和第 14 天， IL-6 表达降低， 造模后第 14 天和第 21 天， TGF-β1 表达降低， 而 3 个时间点 IL-17 表达均降低 （P < 0.05）。结论 五味子提取物可通过抑制 TGF-β1、 IL-6 和 IL-17 的表达来减轻 PQ 中毒造成的炎症反应和肺纤维化。

关键词: 五味子, 百草枯, 肺纤维化, 转化生长因子β1, 白细胞介素-6, 白细胞介素-17

Abstract: Abstract: Objective To explore the protective effcets of Schisandra chinensis extract (SCE) in paraquat (PQ)-induced pulmonary fibrosis in mice，and its intrinsic molecular mechanisms thereof. Methods A total of 108 mice were randomly allocated into six groups (n=18): control group, model group, low concentration of SCE group (200 mg/kg), medium concentration of SCE group (400 mg/kg), high concentration of SCE group (800 mg/kg) and vitamin C group (100 mg/kg). Except control group, mice were given by intragastric administration with PQ (100 mg/kg) and administered with SCE and Vitamin C once per 24 h after PQ modeling. Mice were sacrificed at 7, 14 and 21 d after modeling. Six mice were executed at different time points. The degree of lung tissue inflammation and fibrosis were observed by HE staining and Masson staining. The mRNA and protein expression levels of transforming growth (TGF)-β1, interleukin (IL)-6 and IL-17 in lung tissue were determined by RT-PCR and ELISA respectively. Results (1) Compared with control group, the lung tissue of model group showed a large number of inflammatory cell infiltration, space congestion, and its inflammation scores increased at 7 and 14 days after modeling (P < 0.05). At the same time, compared with model group and vitamin C group, inflammation scores were significantly decreased in medium concentration of SCE group and high concentration of SCE group (P < 0.05). (2) Compared with control group, collagen fibersand the degree of fibrosis were significantly increased in model group， while pulmonary fibrosis were decreased in medium concentration of SCE group and high concentration of SCE group at 14 and 21 days after modeling (P < 0.05). (3) With the extension of modeling time, both mRNA and protein expressions of TGF-β1 were obviously elevated, IL-6 decreased and IL- 17 reduced after the first increase in PQ group. Compared with PQ group, levels of three cytokines mRNA and protein expression in medium concentration of SCE group and high concentration of SCE group changed as follows: IL-6 level was markedly decreased at 7 and 14 days after modeling; TGF-β1 level was markedly increased at 14 and 21 days after modeling. However, IL-17 level was markedly decrease at three time points（P < 0.05） . Conclusion SCE can relieve PQ-induced lung inflammation and fibrosis by suppressing TGF-β1, IL-6, and IL-17 expressions.

Key words: Schisandra chinensis, Paraquat, pulmonary fibrosis, transforming growth factor-β1, interleukin-6, interleu? kin-17