天津医药

天津医药 ›› 2018, Vol. 46 ›› Issue (12): 1286-1290.doi: 10.11958/20180963

• 实验研究 • 上一篇    下一篇

丹酚酸B对糖尿病肾病模型大鼠肾组织中Nrf-2和HO-1的影响

明建松，王晓雪，袁玉华   

  1. 1天津港口医院检验科（邮编300456）；2天津医科大学总医院空港医院检验科
  • 收稿日期:2018-06-20 修回日期:2018-11-02 出版日期:2018-12-15 发布日期:2019-01-24
  • 通讯作者: 袁玉华 E-mail:yyhxxx39@sina.com

Effects of salvianolic acid B on Nrf-2 and HO-1 of renal tissue in rats with diabetic nephropathy

MING Jian-song, WANG Xiao-xue, YUAN Yu-hua   

  1. 1 Department of Clinical Laboratory, Tianjin Port Hospital, Tianjin 300456, China；2 Department of Clinical Laboratory, Tianjin Medical University General Hospital Airport Hospital
  • Received:2018-06-20 Revised:2018-11-02 Published:2018-12-15 Online:2019-01-24

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摘要： 目的 观察不同剂量丹酚酸B对糖尿病肾病模型大鼠肾组织中转录因子NF-E2相关因子2（Nrf-2）和血红素加氧酶1（HO-1）的影响。方法 40只SPF级雄性SD大鼠随机分为对照组（不建模）、模型组、丹酚酸B低剂量组（10 mg/kg）、丹酚酸B高剂量组（20 mg/kg）。采用高糖高脂饮食联合腹腔注射链脲佐菌素（STZ，40 mg/kg）制备大鼠DN模型，建模后丹酚酸B处理组给予相应剂量药物灌胃，对照组及模型组给予等体积橄榄油灌胃，连续给药6周。处理结束后收集各组大鼠24 h尿液，检测24 h尿蛋白含量；取眼球血检测血糖、血肌酐、总胆固醇及三酰甘油水平。 采用免疫蛋白印迹（Western blot）和逆转录PCR（RT-PCR）检测肾组织中Nrf-2、HO-1蛋白及mRNA表达情况，PAS染色观察各组肾组织病变程度。结果 与对照组相比，模型组的24 h尿蛋白、血糖、血肌酐、总胆固醇及三酰甘油含量升高，肾组织中Nrf-2、HO-1蛋白及mRNA的表达量升高，差异均有统计学意义（P＜0.05），肾组织的病变程度加重；与模型组相比，丹酚酸B低、高剂量组的24 h尿蛋白、血糖、血肌酐、总胆固醇及三酰甘油含量明显下降，肾组织中Nrf-2、HO-1蛋白及mRNA的表达量显著升高，差异均有统计学意义（P＜0.05），肾组织的病变程度减轻，且丹酚酸B的效果呈剂量依赖性。结论 一定剂量的丹酚酸B可上调肾组织中Nrf-2及HO-1的表达，抵抗氧化应激。

关键词: 糖尿病肾病, 血红素加氧酶-1, 大鼠, Sprague-Dawley, 丹酚B, 生化指标, 转录因子NF-E2相关因子2

Abstract: Objective To observe the effects of different doses of salvianolic acid B on nuclear transcription factor NFE2-related factor 2 (Nrf-2) and heme oxygenase 1 (HO-1) in kidney of diabetic nephropathy (DN) model rats. Methods Forty SPF male Sprague-Dawley rats were randomly divided into control group (not modeled), model group, low dose of salvianolic acid B (10 mg/kg) and high dose of salvianolic acid B (20 mg/kg) groups. Rats of DN model were prepared by intraperitoneal injection of streptozotocin (STZ, 40 mg / kg) combined with high-sugar and high-fat diet. After modeling,salvianolic acid B treatment group was given the corresponding dose of medicine, and the control group and model group were given equal volume of olive oil intragastrically for 6 weeks. At the end of the treatment, samples of 24-h urine were collected from each group of rats for the detection of 24-h urine protein content. The blood glucose, serum creatinine, total cholesterol and triglyceride levels were measured by eyeball blood. The expressions of Nrf-2 and HO-1 protein and mRNA in renal tissues were detected by reverse transcriptase PCR (RT-PCR) and Western blot assay. PAS staining was used to observe the degree of renal tissue lesion in each group. Results Compared with the control group, the 24-h urine protein,blood glucose, serum creatinine, total cholesterol and triglyceride levels were significantly increased, and the expression levels of Nrf-2, HO-1 protein and mRNA in renal tissues were also significantly increased in the model group (P＜0.05).The pathological changes in renal tissue were significantly increased by PAS staining. Compared with the model group, 24-h urine protein, blood glucose, serum creatinine, total cholesterol and triglyceride levels were significantly decreased in the low and high doses of salvianolic acid B groups. The contents of cholesterol and triglyceride were significantly decreased, and the expression levels of Nrf-2 and HO-1 protein and mRNA in renal tissues were significantly increased (P＜0.05). Thepathological changes in renal tissue were significantly decreased by PAS staining. And the effect of salvianolic acid B was dose dependent. Conclusion A certain dose of salvianolic acid B can up-regulate the expression of Nrf-2 and HO-1 in renal tissues and resist oxidative stress.

Key words: diabetic nephropathies, heme oxygenase-1, rats, Sprague-Dawley, salvianolic acid B, biochemical index, Nrf-2