天津医药

天津医药 ›› 2019, Vol. 47 ›› Issue (4): 400-403.doi: 10.11958/20181158

• 实验研究 • 上一篇    下一篇

背根神经节脉冲射频术对炎性痛大鼠痛阈及脊髓胶质细胞活化的影响

刘靖芷，史可梅，王晓娟，李全波，王慧星，付强，秦丽媛   

  1. 天津医科大学第二医院疼痛治疗中心（邮编300211）
  • 收稿日期:2018-08-01 修回日期:2019-02-28 出版日期:2019-04-15 发布日期:2019-05-27
  • 通讯作者: 刘靖芷 E-mail:ljz77618@126.com
  • 作者简介:刘靖芷（1977），女，博士，副主任医师，主要从事慢性疼痛的微创治疗研究
  • 基金资助:
    国家自然青年科学基金(51303133）;天津市自然科学基金金（09JCYBJC13200）

Effects of pulsed radiofrequency to dorsal root ganglia on pain threshold and the activation of spinal gliocytes in inflammatory pain model rats

LIU Jing-zhi, SHI Ke-mei, WANG Xiao-juan, LI Quan-bo, WANG Hui-xing, FU Qiang, QIN Li-yuan   

  1. Pain Management Center, the Second Hospital of Tianjin Medical University, Tianjin 300211, China
  • Received:2018-08-01 Revised:2019-02-28 Published:2019-04-15 Online:2019-05-27

PDF (PC)

3493

摘要： 目的 评价背根神经节脉冲射频对炎性痛大鼠脊髓星形胶质细胞标记物胶质纤维酸性蛋白（GFAP）、小胶 质细胞标记物补体受体-3的单克隆抗体（OX42）表达的影响。方法 成年雄性 SD大鼠 32只，采用随机数字表法分 为4组（n=8）：对照组（C组）、炎性痛组（IP组）、脉冲射频组（PR组）和炎性痛+脉冲射频组（IP+PR组）。脉冲射频组和 炎性痛+脉冲射频组于造模后第 4天行 L4-5背根神经节脉冲射频术。各组大鼠于造模前（d0）和造模后第 1、3、5、7天 （d1、d3、d5、d7）测定机械缩足反应阈（MWT）和热缩足潜伏期（TWL）。取L4-6脊髓组织，采用Western blot法检测脊髓 GFAP和 OX42的表达。结果 与 C组和与 PR组比较，IP组和 IP+PR组炎性痛造模后各时点 MWT降低，TWL缩短 （P＜0.05）；与 IP组比较，IP+PR组行脉冲射频术后各时点（d5和 d7）MWT升高，TWL延长（P＜0.05）；与 C组和与 PR 组比较，IP组和IP+PR组脊髓GFAP和OX42表达上调（P＜0.05）；与IP组比较，IP+PR组比较脊髓GFAP和OX42表达 降低（P＜0.05）。结论 背根神经节脉冲射频可减轻炎性痛大鼠痛敏反应，抑制炎性痛大鼠脊髓胶质细胞活化。

关键词: 神经节, 脊, 神经元, 炎症, 神经痛, 星形胶质细胞, 小胶质细胞

Abstract: Objective To evaluate the effect of pulsed radiofrequency (PR) of dorsal root ganglion (DRG) on the expression of astrocyte marker GFAP and microglia maker OX42 in spinal cord of rats with inflammatory pain. Methods Thirty-two male SD rats were randomly divided into 4 groups (n=8 for each group), including control group (group C), inflammation pain group (group IP), pulsed radiofrequency group (group PR) and inflammation pain combined with pulsed radiofrequency group (group IP+PR). Pulsed radiofrequency surgery on L4-L5 DRGs was performed at 4 days after IP model. Mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) were measured before (d0) and 1, 3, 5, 7 days (d1, d3, d5, d7) after IP model. The rats were sacrificed after the last measurement of pain threshold, and protein expressions of GFAP and OX42 in the L4-L6 spinal segment in four groups of rats were detected by Western blot assay. Results Compared with group C and group PR, MWT and TWL were decreased in different time points after IP model in group IP and group IP+PR (P＜0.05). Compared with group IP, MWT and TWL were increased in group IP+PR after pulsed radiofrequency (P＜0.05). The protein expressions of GFAP and OX42 were up-regulated in group IP and group IP+PR compared with those of group C and group PR (P＜0.05). Compared with group IP, GFAP and OX42 protein expressions decreased in group IP+PR (P＜0.05). Conclusion The pulsed radiofrequency of DRGs can alleviate the hyperalgesia and inhibit activation of astrocyte and microglia in spinal cord of inflammatory pain rats.

Key words: ganglia, spinal, neurons, inflammation, neuralgia, astrocyte, microglia