天津医药

天津医药 ›› 2022, Vol. 50 ›› Issue (2): 186-190.doi: 10.11958/20211480

• 临床研究 • 上一篇    下一篇

血清TXNIP在2型糖尿病视网膜病变患者中的表达及其与氧化应激的关系

陶琳，赵晓莲 ，齐淑芳
  

  1. 1佳木斯大学基础医学院生理教研室（邮编154007）；2佳木斯市中心医院
  • 收稿日期:2021-06-21 修回日期:2021-11-15 出版日期:2022-02-15 发布日期:2022-02-15
  • 通讯作者: 齐淑芳 E-mail:wangjiamei46987@163.com
  • 作者简介:统计见4修

The expression of serum TXNIP in patients with type 2 diabetic retinopathy and its relationship with oxidative stress

TAO Lin, ZHAO Xiaolian, QI Shufang #br#   

  1. 1 Department of Physiology, School of Basic Medicine, Jiamusi University, Jiamusi 154007, China; 2 The Central of Jiamusi City
  • Received:2021-06-21 Revised:2021-11-15 Published:2022-02-15 Online:2022-02-15

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摘要： 目的 探讨血清硫氧还蛋白互作蛋白（TXNIP）在2型糖尿病视网膜病变（DR）患者中的表达及其与氧化应 激的关系。方法 选取DR患者（DR组）82例，根据疾病严重程度将其分为增生性糖尿病视网膜病变（PDR）组（42 例）以及非增生性糖尿病视网膜病变（NPDR）组（40例）。另选取健康志愿者（对照组）50例以及单纯2型糖尿病患者 （糖尿病组）50例。收集所有研究对象的一般资料。采用酶联免疫吸附试验检测血清TXNIP水平，黄嘌呤氧化酶法 检测超氧化物歧化酶（SOD）水平，二硫代二硝基苯甲酸比色法测定还原型谷胱甘肽（GSH）水平，硫代巴比妥法检测 血清丙二醛（MDA）水平。全自动生化分析仪测定糖化血红蛋白（HbA1c）、空腹血糖（FPG）、三酰甘油（TG）和总胆固 醇（TC）。结果 与对照组比较，糖尿病组和DR组TG、TC升高（P＜0.05），但后2组间差异无统计学意义。对照组、 糖尿病组及 DR 组 HbA1c、FPG、MDA 及 TXNIP 依次增加，而 SOD 和 GSH 依次降低（均 P＜0.05）。与 PDR 组比较， NPDR组MDA和TXNIP降低，而SOD和GSH升高（P＜0.05）；2组HbA1c、FPG、TG及TC差异无统计学意义。较高水 平的 MDA 和 TXNIP 是 DR 患者发生 PDR 的危险因素，而较高水平的 SOD 和 GSH 则是保护因素（P＜0.05）。血清 TXNIP诊断DR的ROC曲线下面积为0.803（95%CI：0.730~0.875），当截断值取3.01 μg/L时，敏感度、特异度和约登指 数分别为 53.66%、94.00% 和 0.477。结论 TXNIP 在 DR 患者血清中呈异常高表达，是 DR 患者发生 PDR 的危险因 素，且可能通过调节氧化应激反应促进疾病的发生、发展，对临床诊断DR有一定辅助价值。

关键词: 糖尿病视网膜病变, 糖尿病, 2型, 氧化性应激, 硫氧还蛋白质类, Logistic模型, ROC曲线, 硫氧还蛋白互作蛋白

Abstract: Objective To investigate the expression of serum thioredoxin-interacting protein (TXNIP) in patients with type 2 diabetic retinopathy (DR) and its relationship with oxidative stress. Methods According to the severity of the disease, 82 DR patients (the DR group) were divided into the PDR group (42 cases) and the NPDR group (40 cases). In addition, 50 healthy volunteers (the control group) and 50 patients with simple type 2 diabetes (the diabetes group) were selected. General data of all subjects were collected. The levels of superoxide dismutase (SOD), reduced glutathione (GSH), malondialdehyde (MDA) and TXNIP were detected by xanthine oxidase method, dithio Dinitrobenzoic acid colorimetry, thiobarbital method and enzyme-linked immunosorbent assay. Glycated hemoglobin (HbA1c), fasting blood glucose (FPG), triglyceride (TG) and total cholesterol (TC) were determined by automatic biochemical analyzer. Results Compared with the control group, TG and TC increased significantly in the diabetes group and the DR group (P＜0.05), but there was no significant difference between the diabetes group and the DR group. HbA1c, FPG, MDA and TXNIP increased sequentially in the control group, the diabetes group and the DR group, while SOD and GSH decreased sequentially (all P＜0.05). Compared with the PDR group, MDA and TXNIP in the NPDR group decreased, while SOD and GSH increased (P＜0.05). There were no significant differences in HbA1c, FPG, TG and TC between the two groups. Higher levels of MDA and TXNIP were all risk factors for PDR in DR patients, higher levels of SOD and GSH were protective factors (P＜0.05). The area under ROC curve of serum TXNIP in the diagnosis of DR was 0.803 (95%CI: 0.730-0.875). When the cutoff value was 3.01 μg/L, the sensitivity, specificity and Youden index were 53.66%, 94.00% and 0.477, respectively. Conclusion TXNIP is abnormally high expressed in the serum of DR patients, and it may promote the occurrence and development of the disease by regulating oxidative stress response. High serum TXNIP level is a risk factor for PDR in DR patients, and serum TXNIP has certain auxiliary value in clinical diagnosis of DR.

Key words: diabetic retinopathy, diabetes mellitus, type 2, oxidative stress, thioredoxins, Logistic models, ROC curve, thioredoxin-interacting protein